479 research outputs found

    A structural role for arginine in proteins: Multiple hydrogen bonds to backbone carbonyl oxygens

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    We propose that arginine side chains often play a previously unappreciated general structural role in the maintenance of tertiary structure in proteins, wherein the positively charged guanidinium group forms multiple hydrogen bonds to backbone carbonyl oxygens. Using as a criterion for a “structural” arginine one that forms 4 or more hydrogen bonds to 3 or more backbone carbonyl oxygens, we have used molecular graphics to locate arginines of interest in 4 proteins: Arg 180 in Thermus thermophilus manganese superoxide dismutase, Arg 254 in human carbonic anhydrase II, Arg 31 in Streptomyces rubiginosus xylose isomerase, and Arg 313 in Rhodospirillum rubrum ribulose‐1,5‐bisphosphate carboxylase/oxygenase. Arg 180 helps to mold the active site channel of superoxide dismutase, whereas in each of the other enzymes the structural arginine is buried in the “mantle” (i.e., inside, but near the surface) of the protein interior well removed from the active site, where it makes 5 hydrogen bonds to 4 backbone carbonyl oxygens. Using a more relaxed criterion of 3 or more hydrogen bonds to 2 or more backbone carbonyl oxygens, arginines that play a potentially important structural role were found in yeast enolase, Bacillus stearothermophilus glyceraldehyde‐3‐phosphate dehydrogenase, bacteriophage T4 and human lysozymes, Enteromorpha prolifera plastocyanin, HIV‐1 protease, Trypanosoma brucei brucei and yeast triosephosphate isomerases, and Escherichia coli trp aporepressor (but not trp repressor or the trp repressor/operator complex). In addition to helping form the active site funnel in superoxide dismutase, the structural arginines found in this study play such diverse roles as stapling together 3 strands of backbone from different regions of the primary sequence, and tying α‐helix to α‐helix, ÎČturn to ÎČ‐turn, and subunit to subunit. Copyright © 1994 The Protein Societ

    Surrogate: A Body-Dexterous Mobile Manipulation Robot with a Tracked Base

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    Robotics platforms in accordance with various embodiments of the invention can be utilized to implement highly dexterous robots capable of whole body motion. Robotics platforms in accordance with one embodiment of the invention include: a memory containing a whole body motion application; a spine, where the spine has seven degrees of freedom and comprises a spine actuator and three spine elbow joints that each include two spine joint actuators; at least one limb, where the at least one limb comprises a limb actuator and three limb elbow joints that each include two limb joint actuators; a tracked base; a connecting structure that connects the at least one limb to the spine; a second connecting structure that connects the spine to the tracked base; wherein the processor is configured by the whole body motion application to move the at least one limb and the spine to perform whole body motion

    Robotics Platforms Incorporating Manipulators Having Common Joint Designs

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    Manipulators in accordance with various embodiments of the invention can be utilized to implement statically stable robots capable of both dexterous manipulation and versatile mobility. Manipulators in accordance with one embodiment of the invention include: an azimuth actuator; three elbow joints that each include two actuators that are offset to allow greater than 360 degree rotation of each joint; a first connecting structure that connects the azimuth actuator and a first of the three elbow joints; a second connecting structure that connects the first elbow joint and a second of the three elbow joints; a third connecting structure that connects the second elbow joint to a third of the three elbow joints; and an end-effector interface connected to the third of the three elbow joints

    Counselor Education and Supervision

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    Abstract: Approaches to mentoring junior faculty in counselor education departments have received minimal attention in the counseling literature. In this article, the authors describe a successful program based on the 10 principles for good mentoring recommended b

    Measurement of the WW Boson Mass

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    A measurement of the mass of the WW boson is presented based on a sample of 5982 W→eÎœW \rightarrow e \nu decays observed in pp‟p\overline{p} collisions at s\sqrt{s} = 1.8~TeV with the D\O\ detector during the 1992--1993 run. From a fit to the transverse mass spectrum, combined with measurements of the ZZ boson mass, the WW boson mass is measured to be MW=80.350±0.140(stat.)±0.165(syst.)±0.160(scale)GeV/c2M_W = 80.350 \pm 0.140 (stat.) \pm 0.165 (syst.) \pm 0.160 (scale) GeV/c^2.Comment: 12 pages, LaTex, style Revtex, including 3 postscript figures (submitted to PRL

    Search for W~1Z~2\widetilde{W}_1\widetilde{Z}_2 Production via Trilepton Final States in ppˉp\bar{p} collisions at s=1.8\sqrt{s}=1.8 TeV

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    We have searched for associated production of the lightest chargino, W~1\widetilde{W}_1, and next-to-lightest neutralino, Z~2\widetilde{Z}_2, of the Minimal Supersymmetric Standard Model in ppˉp\bar{p} collisions at \mbox{s\sqrt{s} = 1.8 TeV} using the \D0 detector at the Fermilab Tevatron collider. Data corresponding to an integrated luminosity of 12.5±0.7\pm 0.7 \ipb were examined for events containing three isolated leptons. No evidence for W~1Z~2\widetilde{W}_1\widetilde{Z}_2 pair production was found. Limits on σ(W~1Z~2)\sigma(\widetilde{W}_1\widetilde{Z}_2)Br(W~1→lÎœZ~1)(\widetilde{W}_1\to l\nu\widetilde{Z}_1)Br(Z~2→llˉZ~1)(\widetilde{Z}_2\to l\bar{l}\widetilde{Z}_1) are presented.Comment: 17 pages (13 + 1 page table + 3 pages figures). 3 PostScript figures will follow in a UUEncoded, gzip'd, tar file. Text in LaTex format. Submitted to Physical Review Letters. Replace comments - Had to resumbmit version with EPSF directive
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