Test of protected silver coating on aluminum samples of ARIEL main telescope mirror substrate material

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Metal-dielectric absorbers with magnetron sputtering technique

International audienc

Proteomic Landscape of Neutrophils in Sickle Cell Anemia: An Unexpected Autoimmune Profile

Meeting: 60th Annual Meeting of the American-Society-of-Hematology (ASH)Location: San Diego, CADate: DEC 01-04, 2018Sponsor: Amer Soc HematolInternational audienceAbstract Although sickle cell disease (SCD) is a red cell disorder, many cell types, including endothelial cells and polymorphonuclear neutrophils (PMNs), contribute to its pathophysiology. In particular, activated PMNs have been implicated to play an important role in the initiation and propagation of vaso-occlusive events in SCD. Activated PMNs engage in a complex process of abnormal interactions with activated endothelial cells, platelets and circulating erythrocytes contributing to endothelial injury and decreased blood flow. In the present study, global proteomic analysis was performed using label-free mass spectrometry of PMNs from 4 SCD patients (SS) in steady state and from 4 control subjects (AA). We identified a total of 4,534 proteins both in AA and SS PMNs with 3,080 of these proteins identified in at least three samples for each condition. 50 proteins were significantly over-expressed in SS PMNs compared to AA PMNs (ratio > 1.4). STRING employed to monitor potential interaction between the overexpressed proteins showed that the main interactive clusters consist of STAT1 and STAT2, OAS 1, 2 and 3, and many Interferon Signaling Proteins i.e. IFIT1, IFIT2, IFIT 3, ISG15, ISG20, GBP2, IFI35, MX1 and MX2, TLR8 proteins (Fig. 1). This finding implies a strong activation of the type I interferon (IFN) signaling pathway in the SS PMNs (between 10 and 100-fold increase in SS vs AA). In addition, 33 proteins showed significantly lower expression in SS PMNs compared to AA PMNs. Among these were L-selectin (CD62L) and IL-17 receptor A (IL17RA) (p = 0.01). These findings are consistent with previously described phenotypes of aged neutrophils and acute inflammatory responses in SCD. Similar proteomic analysis performed on PMNs from SS patients treated with hydroxycarbamide (HC, n=4) showed that 14 proteins had significantly lower expression compared to untreated-SS patients (ratio <0.7). Interestingly, HC restored a normal expression pattern for most of the interferon signaling proteins. Type I IFNs represent the major effector cytokines of the host immune response against viruses and other intracellular pathogens. These cytokines are produced via activation of STAT1 and of pattern recognition receptors, including the Toll-like receptor signaling network. To determine if type I IFN-α could be detected at the protein level in the plasma of SS patients, we used the novel digital-ELISA technology (SIMOA, Quanterix) developed by Wilson et al (J Lab Autom, 2016). Interestingly, we found an increased level of INFα in plasma from SS patients compared to AA (n=32) (p<0.001) and it is noteworthy that while 50% of SS patients have similar level of INFα compare to AA individuals the other 50% exhibit 10 to 1,000-fold increased levels (Fig. 2). In summary, our novel proteomic analysis documents a high level expression of interferon signaling proteins, STAT1 and TLR8 in the proteome of neutrophils from SS patients and strongly suggests autoimmune or auto-inflammatory phenomena at basal state in SCD. Our results provide strong support for an important role for the innate immune system in the pathophysiology of SCD. Future studies will help determine the relationship between the plasmatic level of IFN-α and clinical complications and will establish if interferon signaling proteins and IFN-α could represent new therapeutic targets in SCD. Disclosures Hermand-Tournamille: Imara: Research Funding. Le Va Kim:Imara: Research Funding. Koehl:Imara: Research Funding

Absorbing Coating in Magnetron Sputtering for Parasitic Light Reduction

International audienc

Qualification of the thermal stabilization, polishing and coating procedures for the aluminum telescope mirrors of the ARIEL mission

ARIEL, the Atmospheric Remote-sensing Infrared Exoplanet Large-survey, was selected as the fourth medium-class mission in ESA's Cosmic Vision program. ARIEL is based on a 1 m class telescope optimized for spectroscopy in the waveband between 1.95 and 7.8 micron and operating in cryogenic conditions. Fabrication of the 1.1 m aluminum primary mirror for the ARIEL telescope requires technological advances in the three areas of substrate thermal stabilization, optical surface polishing and coating. This article describes the qualification of the three procedures that have been set up and tested to demonstrate the readiness level of the technological processes employed. Substrate thermal stabilization is required to avoid deformations of the optical surface during cool down of the telescope to the operating temperature below 50 K. Purpose of the process is to release internal stress in the substrate that can cause such shape deformations. Polishing of large aluminum surfaces to optical quality is notoriously difficult due to softness of the material, and required setup and test of a specific polishing recipe capable of reducing residual surface shape errors while maintaining surface roughness below 10 nm RMS. Finally, optical coating with protected silver must be qualified for environmental stability, particularly at cryogenic temperatures, and uniformity. All processes described in this article have been applied to aluminum samples of up to 150 mm of diameter, leading the way to the planned final test on a full size demonstrator of the ARIEL primary mirror