85 research outputs found
Organizational change, psychosocial work environment, and non-disability early retirement:a prospective study among senior public employees
Bit errors in the Kirchhoff-Law–Johnson-Noise secure key exchange
Background: Accuracy in malaria diagnosis and staging is vital in order to reduce mortality and post infectious sequelae. Herein we present a metabolomics approach to diagnostic staging of malaria infection, specifically Plasmodium falciparum infection in children. Methods: A group of 421 patients between six months and six years of age with mild and severe states of malaria with age-matched controls were included in the study, 107, 192 and 122 individuals respectively. A multivariate design was used as basis for representative selection of twenty patients in each category. Patient plasma was subjected to Gas Chromatography-Mass Spectrometry analysis and a full metabolite profile was produced from each patient. In addition, a proof-of-concept model was tested in a Plasmodium berghei in-vivo model where metabolic profiles were discernible over time of infection. Results: A two-component principal component analysis (PCA) revealed that the patients could be separated into disease categories according to metabolite profiles, independently of any clinical information. Furthermore, two sub-groups could be identified in the mild malaria cohort who we believe represent patients with divergent prognoses. Conclusion: Metabolite signature profiling could be used both for decision support in disease staging and prognostication
Dual impact of organisational change on subsequent exit from work unit and sickness absence:A longitudinal study among public healthcare employees
Longitudinal associations between organizational change, work-unit social capital, and employee exit from the work unit among public healthcare workers:A mediation analysis
Work-unit organisational changes and subsequent prescriptions for psychotropic medication:a longitudinal study among public healthcare employees
Influence of errors in job codes on job exposure matrix-based exposure assessment in the register-based occupational cohort DOC*X
Acute myocardial infarction in relation to physical activities at work:a nationwide follow-up study based on job-exposure matrices
Are risk estimates biased in follow-up studies of psychosocial factors with low base-line participation?
One-Step Purification of Recombinant Human Amelogenin and Use of Amelogenin as a Fusion Partner
Amelogenin is an extracellular protein first identified as a matrix component important for formation of dental enamel during tooth development. Lately, amelogenin has also been found to have positive effects on clinical important areas, such as treatment of periodontal defects, wound healing, and bone regeneration. Here we present a simple method for purification of recombinant human amelogenin expressed in Escherichia coli, based on the solubility properties of amelogenin. The method combines cell lysis with recovery/purification of the protein and generates a >95% pure amelogenin in one step using intact harvested cells as starting material. By using amelogenin as a fusion partner we could further demonstrate that the same method also be can explored to purify other target proteins/peptides in an effective manner. For instance, a fusion between the clinically used protein PTH (parathyroid hormone) and amelogenin was successfully expressed and purified, and the amelogenin part could be removed from PTH by using a site-specific protease
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