101 research outputs found
ΠΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΠ΅ Π²ΠΎΠ΄Ρ ΠΈ Π²ΠΎΠ΄Π½ΡΡ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠ² Π³Π»ΠΈΠ½ΠΎΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΠΌΠΈ Π½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ°ΠΌΠΈ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΡ
Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½ΠΎ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠ΅ ΠΈ ΠΏΠΎΠ²ΡΠΎΡΠ½ΠΎΠ΅ ΠΏΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΠ΅ Π²ΠΎΠ΄Ρ ΠΈ ΡΠ°ΡΡΠ²ΠΎΡΠ°, ΠΈΠΌΠΈΡΠΈΡΡΡΡΠ΅Π³ΠΎ ΠΆΠΈΠ΄ΠΊΠΈΠ΅ ΠΎΡΡ
ΠΎΠ΄Ρ Π°ΡΠΎΠΌΠ½ΡΡ
ΡΡΠ°Π½ΡΠΈΠΉ, Π³Π»ΠΈΠ½ΠΎΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΠΌΠΈ Π½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ°ΠΌΠΈ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΡ, ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π±Π΅Π½ΡΠΎΠ½ΠΈΡΠ°. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ,
ΡΡΠΎ ΡΠ°ΡΡΠΈΡΡ Π³Π»ΠΈΠ½ΠΎΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ° Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΠΏΠΎΠ³Π»ΠΎΡΠ°ΡΡ Π²ΠΎΠ΄Ρ ΠΈ ΡΠΎΠ»Π΅Π²ΡΠ΅ ΡΠ°ΡΡΠ²ΠΎΡΡ ΠΏΠΎ
ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΡΠ°ΡΡΠΈΡΠ°ΠΌΠΈ ΠΏΡΠΈΡΠΎΠ΄Π½ΠΎΠ³ΠΎ Π±Π΅Π½ΡΠΎΠ½ΠΈΡΠ°.Π ΠΎΠ·Π³Π»ΡΠ½ΡΡΠΎ ΠΏΠ΅ΡΠ²ΠΈΠ½Π½Π΅ Ρ ΠΏΠΎΠ²ΡΠΎΡΠ½Π΅ ΠΏΠΎΠ³Π»ΠΈΠ½Π°Π½Π½Ρ Π²ΠΎΠ΄ΠΈ Ρ
ΡΠΎΠ·ΡΠΈΠ½Ρ, ΡΠΊΠΈΠΉ ΡΠΌΡΡΡΡ ΡΡΠ΄ΠΊΡ Π²ΡΠ΄Ρ
ΠΎΠ΄ΠΈ Π°ΡΠΎΠΌΠ½ΠΈΡ
ΡΡΠ°Π½ΡΡΠΉ,
Π³Π»ΠΈΠ½ΠΎΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ½ΠΈΠΌΠΈ Π½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ°ΠΌΠΈ ΠΏΡΠΎΠ½ΠΈΠΊΠ½Π΅Π½Π½Ρ,
ΡΠΈΠ½ΡΠ΅Π·ΠΎΠ²Π°Π½ΠΈΠΌΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Ρ Π±Π΅Π½ΡΠΎΠ½ΡΡΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΠΎ ΡΠ°ΡΡΠΈΠ½ΠΊΠΈ Π³Π»ΠΈΠ½ΠΎΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ° Π΅ΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡΠ΅ ΠΏΠΎΠ³Π»ΠΈΠ½Π°ΡΡΡ Π²ΠΎΠ΄Ρ Ρ ΡΠΎΠ»ΡΠΎΠ²Ρ ΡΠΎΠ·ΡΠΈΠ½ΠΈ Ρ ΠΏΠΎΡΡΠ²Π½ΡΠ½Π½Ρ Π· ΡΠ°ΡΡΠΈΠ½ΠΊΠ°ΠΌΠΈ ΠΏΡΠΈΡΠΎΠ΄Π½ΠΎΠ³ΠΎ Π±Π΅Π½ΡΠΎΠ½ΡΡΡ.In many cases the use of nanocomposites is restrained by
insufficient knowledge of their properties. It primarily
relates to intercalated nanocomposites, especially the nano
composites with particles size less than 0.6 mm, containing
a significant amount of clay (>60 %). The research
is focus on the study of the properties of such na nocomposites.
The bentonite clay from the Cherkassy deposit
comprising up to 95 % of Ca-montmorillonite was used
for the synthesis of nanocomposites. The preliminary
substitution of NaβΊ cations for CaΒ²βΊ and MgΒ²βΊ cations was
carried out. The nanocomposites clay-polymer were obtained in the process of free radical polymerization. The
nanocomposites, dried at 105 ΒΊC with particle size of 0.4 Γ·
0.6 mm were used for experiments. The properties of the nanocomposites to take up
water and solutions were studied by Enslin method. The
maximum value of water uptake was 69.45 ml/g. Subsequently,
the sample was dried on the filter to constant weight and repeated water uptake was examined. In this
case, the result was 82 ml/g, i. e. in repeated contact with
water particles absorbed by 18 % more amount water than
during first water uptake. The increase in water uptake is
due to nonuniform polymerization (in volume and time),
which resulted in deformation of some polymer chains and
only after water absorption, polymer chains have a chance
to take a more low-energy position. Since this phenomenon
was observed for particle size of less than 0.6 mm, it can
be assumed that the crystallites or aggregates of intercalated
nanocomposites clay-polymer are the sites of the beginning
of acrylamide polymerization. The rate of water re-absorption was lower than in
case of the primary water uptake up to period of time of
1000 min and was constant to 5000 min while the rate of
primary absorption decreased substantially after 1000 min.
The sorption capacity of nanocomposites for solutions
simulating waste of nuclear power plants was 18 ml/g that was higher than absorption by natural bentonite (3.4 ml/g). Thus, the intercalated nanocomposites clay-polymer
could be promising materials as components of the geochemical and water insulating barriers
A Two-Site Immunoradiometric Assay for Serum Calcitonin Using Monoclonal Anti-Peptide Antibodies
We have produced a library of monoclonal antibodies of various affinities by immunizing mice with synthetic calcitonin (CT) 1-32. These monoclonal antibodies defined two antigenic determinants on the molecule of CT. The first was located in the 11-17 region of the hormone: the second was present on the 26-32 portion of CT. The C-terminal epitope was restricted to the mature form of the hormone and immunologically silent on synthetic peptides with sequences analogous lo the biosynthetic precursors for CT. Using two high-affinity monoclonal antibodies, designated as CT07 and CT08, we developed a two-site immunoradiometric assay (m-lRMA) for serum CT. This m-lRMA provided a sensitivity of 10 pg/mL using a one-step overnight incubation at room temperature. Gel filtration analyses of serum samples from patients with medullary thyroid carcinoma (MTC) demonstrated that the CT07-CT08 m-lRMA was specific for the circulating mature form of CT
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