Afferent Visual Pathway Affection in Patients with PMP22 Deletion-Related Hereditary Neuropathy with Liability to Pressure Palsies

Background The PMP22 gene encodes a protein integral to peripheral myelin. Its deletion leads to hereditary neuropathy with liability to pressure palsies (HNPP). PMP22 is not expressed in the adult central nervous system, but previous studies suggest a role in CNS myelin development. The objective of this study was to identify potential structural and functional alterations in the afferent visual system in HNPP patients. Methods Twenty HNPP patients and 18 matched healthy controls (HC) were recruited in a cross-sectional study. Participants underwent neurological examination including visual acuity, visual evoked potential (VEP) examination, optical coherence tomography (OCT), and magnetic resonance imaging with calculation of brain atrophy, regarding grey and white matter, and voxel based morphometry (VBM), in addition answered the National Eye Institute’s 39-item Visual Functioning Questionnaire (NEI- VFQ). Thirteen patients and 6 HC were additionally examined with magnetic resonance spectroscopy (MRS). Results All patients had normal visual acuity, but reported reduced peripheral vision in comparison to HC in the NEI-VFQ (p = 0.036). VEP latency was prolonged in patients (P100 = 103.7±5.7 ms) in comparison to healthy subjects (P100 = 99.7±4.2 ms, p = 0.007). In OCT, peripapillary retinal nerve fiber layer thickness RNFL was decreased in the nasal sector (90.0±15.5 vs. 101.8±16.5, p = 0.013), and lower nasal sector RNFL correlated with prolonged VEP latency (Rho = -0.405, p = 0.012). MRS revealed reduced tNAA (731.4±45.4 vs. 814.9±62.1, p = 0.017) and tCr (373.8±22.2 vs. 418.7±31.1, p = 0.002) in the visual cortex in patients vs. HC. Whole brain volume, grey and white matter volume, VBM and metabolites in a MRS sensory cortex control voxel did not differ significantly between patients and HC. Conclusion PMP22 deletion leads to functional, metabolic and macro- structural alterations in the afferent visual system of HNPP patients. Our data suggest a functional relevance of these changes for peripheral vision, which warrants further investigation and confirmation

Therapeutic melanoma inhibition by local micelle-mediated cyclic nucleotide repression

The acidic tumor microenvironment in melanoma drives immune evasion by up-regulating cyclic adenosine monophosphate (cAMP) in tumor-infiltrating monocytes. Here we show that the release of non-toxic concentrations of an adenylate cyclase (AC) inhibitor from poly(sarcosine)-block-poly(L-glutamic acid γ-benzyl ester) (polypept(o)id) copolymer micelles restores antitumor immunity. In combination with selective, non-therapeutic regulatory T cell depletion, AC inhibitor micelles achieve a complete remission of established B16-F10-OVA tumors. Single-cell sequencing of melanoma-infiltrating immune cells shows that AC inhibitor micelles reduce the number of anti-inflammatory myeloid cells and checkpoint receptor expression on T cells. AC inhibitor micelles thus represent an immunotherapeutic measure to counteract melanoma immune escape.Drug Delivery Technolog

De Medici Officio Dissertatio Secunda

Quam Praeside Johanne Bohnio, Medic. Doct. & Prof. Publ. Disquisitioni Philiatrorum exponet Johannes Brasche/ Lubecens. Medic. Candid. In Auditorio Maiore, D. 9. Aprilis 1697

D. Johannis Bohn/ Anat. & Chir. Prof. Publ. De Renunciatione Vulnerum, Seu Vulnerum Lethalium Examen : Exponens horum Formalitatem & Causas, tam in genere, quam in specie ac per singulas Corporis partes

Vorlageform d. Erscheinungsvermerks: Lipsiae, Sumtibus Joh. Frid. Gleditsch. Typis Christophori Fleischeri, M.DC.LXXXIX

Lectori Benevolo Facultatis Medicae In Academia Lipsiensi p. t. Procancellarius D. Johannes Bohn, Therap. Prof. Pub. S. & O. : [P. P. Lipsiae d. 7. Iulii 1698.]

Gelegenheitsschrift auf Abraham HoffmannErscheinungsjahr nach der Datierung am Ende des Textes angegebe

Lectori Benevolo Subiecti Muliebris Resectionem Publicam intimat, Eundemque ad hanc perhumaniter invitat D. Johann Bohn/ Anat. & Chir. P.P. : [Lips. 11. Novembr. MDCLXXXVII.]

Erscheinungsjahr der Datierung am Ende des Textes entnomme

D. Johannis Bohn/ Prof. Publ. Meditationes Physico-Chymicae De Aerisin In Sublunaria Influxu : Sc. hunc neque secundum Peripatheticos, nec, Chymicos, materialem, sed formalem saltem, videri

Auch in: D. Johannis Bohn/ Professoris in Academia Lipsiensi Publici, Dissertationes Chymico-Physica

Lectori Benevolo Panegyrin Facultatis Medicæ In Academia Lipsiensi Indicat Hujus p.t. Procancellarius D. Iohannes Bohn, Prof. Publ. Inque Præsenti Programm. Inaugurali Disqvirit: An Medici sub Conscientiæ & Existimationis suæ integritate a Pharmacorum Confectione & exhibitione abstinere qveant? i.e. Ob die Medici ohne Verletzung ihres Gewissens und Ehre sich der Præparation und Dispensation der Medicamentorum enthalten, oder selbe unterlassen können? : [P. P. Lipsiæ d. IV. Septembr. M.DCCX.]

Autopsie nach Ex. der ULB Sachsen-AnhaltVorlageform des Kolophons: Lipsiæ, Literis Brandenburgerianis. - Erscheinungsjahr der Datierung am Schluß entnommenEinladung zur Verteidigung der med. Diss. von Sigismund Schmieder, 4. Sept.171

De Medici Officio Dissertatio Prima

Quam Praeside Johanne Bohnio, Med. Doct. & Prof. Publ. Disquisitioni Philiatrorum exponet M. Joh. Christianus Lehmann/ Budissin. Med. Baccal. In Auditorio Maiori D. 12. Febr. 1697.Nicht identisch mit VD17 7:681009U (dort keine Widmung auf der Rücks. des Titelbl.