8 research outputs found
Transition numĂ©rique dâun cours dâintroduction au marketing : conception dâun dispositif dâenseignement mixte adaptĂ© Ă la mĂ©thode des Ă©tudes de cas
Dans ce compte rendu de pratique intĂ©grant les technologies de lâinformation, nous prĂ©sentons la façon dont nous avons adaptĂ© la prĂ©paration des travaux pratiques dâun cours dâintroduction au marketing. Nous avons utilisĂ© sept outils numĂ©riques diffĂ©rents afin de rendre cette prĂ©paration plus personnalisĂ©e, participative et interactive. Cette innovation pĂ©dagogique a fait lâobjet dâune Ă©valuation de la part des Ă©tudiants et Ă©tudiantes, de lâĂ©quipe enseignante et dâexperts indĂ©pendants. Ce compte rendu sâappuie sur cette triple Ă©valuation pour Ă©tablir les forces, les faiblesses et les pistes dâamĂ©lioration future de ce dispositif dâapprentissage.In this practice report on integrating information technologies, we show how the way in which we adapted the preparation for the practical exercises of an introductory marketing course. We used seven different digital tools to make the work more personalized, engaging and interactive. This pedagogical innovation was evaluated by the students, the teaching team and independent experts. On the basis of this three-fold assessment, this report identifies the strengths, weaknesses and areas for future improvement of this learning aid
Large diversity of linezolid-resistant isolates discovered in food-producing animals through linezolid selective monitoring in Belgium in 2019.
Linezolid is a critically important antibiotic used to treat human infections caused by MRSA and VRE. While linezolid is not licensed for food-producing animals, linezolid-resistant (LR) isolates have been reported in European countries, including Belgium. To: (i) assess LR occurrence in staphylococci and enterococci isolated from different Belgian food-producing animals in 2019 through selective monitoring; and (ii) investigate the genomes and relatedness of these isolates. Faecal samples (nâ=â1325) and nasal swab samples (nâ=â148) were analysed with a protocol designed to select LR bacteria, including a 44-48âh incubation period. The presence of LR chromosomal mutations, transferable LR genes and their genetic organizations and other resistance genes, as well as LR isolate relatedness (from this study and the NCBI database) were assessed through WGS. The LR rate differed widely between animal host species, with the highest rates occurring in nasal samples from pigs and sows (25.7% and 20.5%, respectively) and faecal samples from veal calves (16.4%). WGS results showed that LR determinants are present in a large diversity of isolates circulating in the agricultural sector, with some isolates closely related to human isolates, posing a human health risk. LR dedicated monitoring with WGS analysis could help to better understand the spread of LR. Cross-selection of LR transferable genes through other antibiotic use should be considered in future action plans aimed at combatting antimicrobial resistance and in future objectives for the rational use of antibiotics in a One Health perspective
Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains.
Accurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistant Enterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays against Enterobacterales challenging strains. Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEKÂź 2 (nâ=â5) and BD Phoenixâą (nâ=â3) automated systems, ETESTÂź gradient strip (nâ=â3), and disk (3 brands) diffusion method (DD; nâ=â6) for temocillin susceptibility using standardized methodology. Results were interpreted using EUCAST 2023 criteria and compared to the broth microdilution (BMD; Sensititreâą panel) method used as gold standard. Methodsâ reproducibility was assessed by testing 3 reference strains in triplicate. A total of 702 organism-drug results were obtained against 33 TMO-susceptible and 14 TMO-resistant isolates. Excluding Proteae species (P. mirabilis and M. morganii), the essential agreement rates were excellent (91.5-100%) for all MIC-based methods. The highest category agreement was achieved by ETESTÂź (97.5%) followed by VITEKÂź 2 (93.2%), disk diffusion (91.6%), and BD Phoenixâą (88.5%). BD Phoenixâą and paper disk diffusion overcalled resistance (11.5% and 6.8% of major discrepancies, respectively), while ROSCO tablets diffusion and VITEKÂź 2 generated higher very major discrepancies (7.1% and 4.2% respectively). Inter-assay reproducibility was unsatisfactory using recommended E. coli ATCC 25922 strain but was excellent with E. coli ATCC 35218 and K. pneumoniae ATCC 700603 strains. This interlaboratory study suggests that routine testing methods provide accurate and reproducible TMO categorization results except for Proteae species.</p
Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains.
Accurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistant Enterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays against Enterobacterales challenging strains. Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEKÂź 2 (nâ=â5) and BD Phoenixâą (nâ=â3) automated systems, ETESTÂź gradient strip (nâ=â3), and disk (3 brands) diffusion method (DD; nâ=â6) for temocillin susceptibility using standardized methodology. Results were interpreted using EUCAST 2023 criteria and compared to the broth microdilution (BMD; Sensititreâą panel) method used as gold standard. Methods' reproducibility was assessed by testing 3 reference strains in triplicate. A total of 702 organism-drug results were obtained against 33 TMO-susceptible and 14 TMO-resistant isolates. Excluding Proteae species (P. mirabilis and M. morganii), the essential agreement rates were excellent (91.5-100%) for all MIC-based methods. The highest category agreement was achieved by ETESTÂź (97.5%) followed by VITEKÂź 2 (93.2%), disk diffusion (91.6%), and BD Phoenixâą (88.5%). BD Phoenixâą and paper disk diffusion overcalled resistance (11.5% and 6.8% of major discrepancies, respectively), while ROSCO tablets diffusion and VITEKÂź 2 generated higher very major discrepancies (7.1% and 4.2% respectively). Inter-assay reproducibility was unsatisfactory using recommended E. coli ATCC 25922 strain but was excellent with E. coli ATCC 35218 and K. pneumoniae ATCC 700603 strains. This interlaboratory study suggests that routine testing methods provide accurate and reproducible TMO categorization results except for Proteae species