4,542 research outputs found
Determination of the Her-2/neu gene amplification status in cytologic breast cancer specimens using automated silver-enhanced in-situ hybridization (SISH)
Silver-enhanced in-situ hybridization (SISH) is an emerging tool for the determination of the Her-2/neu amplification status in breast cancer. SISH is technically comparable to fluorescence in-situ hybridization (FISH) but does not require a fluorescence microscope for its interpretation. Although recent studies on histologic evaluations of SISH are promising, we aimed to evaluate its performance on 71 cytologic breast cancer specimens with the new combined Her-2/Chr17 probe. Her-2/neu status as routinely determined by FISH was available for all patients. We found SISH signals in cytologic cell blocks and smear specimens easy to evaluate in most cases. Small numbers of tumor cells and difficulties in identifying tumor cells in lymphocyte-rich backgrounds were limiting factors. Her-2/neu status, as determined by Her-2/Chr17 SISH, was basically identical to the results of the corresponding FISH. The discrepancies were mainly owing to the heterogeneity of Her-2/neu amplification in the tumor tissue. Interobserver agreement for the SISH evaluation was high (kappa value: 0.972). We conclude that Her-2/Chr17 SISH is a useful and accurate method for the evaluation of the Her-2/neu gene amplification status in cytologic breast cancer specimens, particularly in metastatic breast cancer lesions. The advantages of signal permanency and bright-field microscopic result interpretation make this technique an attractive alternative to the current FISH-based gold standard
Evolution of the Cluster Correlation Function
We study the evolution of the cluster correlation function and its
richness-dependence from z = 0 to z = 3 using large-scale cosmological
simulations. A standard flat LCDM model with \Omega_m = 0.3 and, for
comparison, a tilted \Omega_m = 1 model, TSCDM, are used. The evolutionary
predictions are presented in a format suitable for direct comparisons with
observations. We find that the cluster correlation strength increases with
redshift: high redshift clusters are clustered more strongly (in comoving
scale) than low redshift clusters of the same mass. The increased correlations
with redshift, in spite of the decreasing mass correlation strength, is caused
by the strong increase in cluster bias with redshift: clusters represent higher
density peaks of the mass distribution as the redshift increases. The
richness-dependent cluster correlation function, presented as the
correlation-scale versus cluster mean separation relation, R_0 - d, is found to
be, remarkably, independent of redshift to z <~ 2 for LCDM and z <~ 1 for TCDM
(for a fixed correlation function slope and cluster mass within a fixed
comoving radius). The non-evolving R_0 - d relation implies that both the
comoving clustering scale and the cluster mean separation increase with
redshift for the same mass clusters so that the R_0 - d relation remains
essentially unchanged. The evolution of the R_0 - d relation from z ~ 0 to z ~
3 provides an important new tool in cosmology; it can be used to break
degeneracies that exist at z ~ 0 and provide precise determination of
cosmological parameters.Comment: AASTeX, 15 pages, including 5 figures, accepted version for
publication in ApJ, vol.603, March 200
Chandra detection of extended X-ray emission from the recurrent nova RS Ophiuchi
Radio, infrared, and optical observations of the 2006 eruption of the
symbiotic recurrent nova RS Ophiuchi (RS Oph) showed that the explosion
produced non-spherical ejecta. Some of this ejected material was in the form of
bipolar jets to the east and west of the central source. Here we describe Xray
observations taken with the Chandra X-ray Observatory one and a half years
after the beginning of the outburst that reveal narrow, extended structure with
a position angle of approximately 300 degrees (east of north). Although the
orientation of the extended feature in the X-ray image is consistent with the
readout direction of the CCD detector, extensive testing suggests that the
feature is not an artifact. Assuming it is not an instrumental effect, the
extended X-ray structure shows hot plasma stretching more than 1,900 AU from
the central binary (taking a distance of 1.6 kpc). The X-ray emission is
elongated in the northwest direction - in line with the extended infrared
emission and some minor features in the published radio image. It is less
consistent with the orientation of the radio jets and the main bipolar optical
structure. Most of the photons in the extended X-ray structure have energies of
less than 0.8 keV. If the extended X-ray feature was produced when the nova
explosion occurred, then its 1".2 length as of 2007 August implies that it
expanded at an average rate of more than 2 mas/d, which corresponds to a flow
speed of greater than 6,000 km/s (d/1.6 kpc) in the plane of the sky. This
expansion rate is similar to the earliest measured expansion rates for the
radio jets.Comment: accepted in Ap
The Critical Hopping Parameter in O(a) improved Lattice QCD
We calculate the critical value of the hopping parameter, , in O(a)
improved Lattice QCD, to two loops in perturbation theory. We employ the
Sheikholeslami-Wohlert (clover) improved action for Wilson fermions.
The quantity which we study is a typical case of a vacuum expectation value
resulting in an additive renormalization; as such, it is characterized by a
power (linear) divergence in the lattice spacing, and its calculation lies at
the limits of applicability of perturbation theory.
The dependence of our results on the number of colors , the number of
fermionic flavors , and the clover parameter , is shown
explicitly. We compare our results to non perturbative evaluations of
coming from Monte Carlo simulations.Comment: 11 pages, 2 EPS figures. The only change with respect to the original
version is inclusion of the standard formulae for the gauge fixing and ghost
parts of the action. Accepted for publication in Physical Review
Dynamical overlap fermions, results with hybrid Monte-Carlo algorithm
We present first, exploratory results of a hybrid Monte-Carlo algorithm for
dynamical, n_f=2, four-dimensional QCD with overlap fermions. As expected, the
computational requirements are typically two orders of magnitude larger for the
dynamical overlap formalism than for the more conventional (Wilson or
staggered) formulations.Comment: 13 pages, 2 figure
Cystatins as calpain inhibitors: Engineered chicken cystatin- and stefin B-kininogen domain 2 hybrids support a cystatin-like mode of interaction with the catalytic subunit of ÎĽ-calpain
Within the cystatin superfamily, only kininogen domain 2 (KD2) is able to inhibit ÎĽ- and m-calpain. In an attempt to elucidate the structural requirements of cystatins for calpain inhibition, we constructed recombinant hybrids of human stefin B (an intracellular family 1 cystatin) with KD2 and Delta L110 deletion mutants of chicken cystatin-KD2 hybrids. Substitution of the N-terminal contact region of stefin B by the corresponding KD2 sequence resulted in a calpain inhibitor of K-i = 188 nM. Deletion of L110, which forms a beta -bulge in family 1 and 2 cystatins but is lacking in KD2, improved inhibition of mu -calpain 4- to 8-fold. All engineered cystatins were temporary inhibitors of calpain due to slow substrate-like cleavage of a single peptide bond corresponding to Gly9-Ala10 in chicken cystatin. Biomolecular interaction analysis revealed that, unlike calpastatin, the cystatin-type inhibitors do not bind to the calmodulin-like domain of the small subunit of calpain, and their interaction with the mu -calpain heterodimer is completely prevented by a synthetic peptide comprising subdomain B of calpastatin domain 1. Based on these results we propose that (i) cystatin-type calpain inhibitors interact with the active site of the catalytic domain of calpain in a similar cystatin-like mode as with papain and (ii) the potential for calpain inhibition is due to specific subsites within the papain-binding regions of the general cystatin fold
Swift observations of the 2006 outburst of the recurrent nova RS Ophiuchi: II. 1D hydrodynamical models of wind driven shocks
Following the early Swift X-ray observations of the latest outburst of the
recurrent nova RS Ophiuchi in February 2006 (Paper I), we present new 1D
hydrodynamical models of the system which take into account all three phases of
the remnant evolution. The models suggest a novel way of modelling the system
by treating the outburst as a sudden increase then decrease in wind mass-loss
rate and velocity. The differences between this wind model and previous
Primakoff-type simulations are described. A more complex structure, even in 1D,
is revealed through the presence of both forward and reverse shocks, with a
separating contact discontinuity. The effects of radiative cooling are
investigated and key outburst parameters such as mass-loss rate, ejecta
velocity and mass are varied. The shock velocities as a function of time are
compared to the ones derived in Paper I. We show how the manner in which the
matter is ejected controls the evolution of the shock and that for a
well-cooled remnant, the shock deceleration rate depends on the amount of
energy that is radiated away.Comment: 9 pages, 5 figure
Supermassive Black Holes and Galaxy Formation
The formation of supermassive black holes (SMBH) is intimately related to
galaxy formation, although precisely how remains a mystery. I speculate that
formation of, and feedback from, SMBH may alleviate problems that have arisen
in our understanding of the cores of dark halos of galaxies.Comment: Talk at conference on Matter in the Universe, March 2001, ISSI Ber
String amplitudes in arbitrary dimensions
We calculate gravitational dressed tachyon correlators in non critcal
dimensions. The 2D gravity part of our theory is constrained to constant
curvature. Then scaling dimensions of gravitational dressed vertex operators
are equal to their bare conformal dimensions. Considering the model as d+2
dimensional critical string we calculate poles of generalized Shapiro-Virasoro
amplitudes.Comment: 14 page
Quantitative analysis of cell types during growth and morphogenesis in Hydra
Tissue maceration was used to determine the absolute number and the distribution of cell types in Hydra. It was shown that the total number of cells per animal as well as the distribution of cells vary depending on temperature, feeding conditions, and state of growth. During head and foot regeneration and during budding the first detectable change in the cell distribution is an increase in the number of nerve cells at the site of morphogenesis. These results and the finding that nerve cells are most concentrated in the head region, diminishing in density down the body column, are discussed in relation to tissue polarity
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