59 research outputs found
Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization.
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms
Experimental Study of the Shortest Reset Word of Random Automata
In this paper we describe an approach to finding the shortest reset word of a
finite synchronizing automaton by using a SAT solver. We use this approach to
perform an experimental study of the length of the shortest reset word of a
finite synchronizing automaton. The largest automata we considered had 100
states. The results of the experiments allow us to formulate a hypothesis that
the length of the shortest reset word of a random finite automaton with
states and 2 input letters with high probability is sublinear with respect to
and can be estimated as $1.95 n^{0.55}.
Two-proton correlations from 158 AGeV Pb+Pb central collisions
The two-proton correlation function at midrapidity from Pb+Pb central
collisions at 158 AGeV has been measured by the NA49 experiment. The results
are compared to model predictions from static thermal Gaussian proton source
distributions and transport models RQMD and VENUS. An effective proton source
size is determined by minimizing CHI-square/ndf between the correlation
functions of the data and those calculated for the Gaussian sources, yielding
3.85 +-0.15(stat.) +0.60-0.25(syst.) fm. Both the RQMD and the VENUS model are
consistent with the data within the error in the correlation peak region.Comment: RevTeX style, 6 pages, 4 figures, 1 table. More discussion are added
about the structure on the tail of the correlation function. The systematic
error is revised. To appear in Phys. Lett.
Event-by-event fluctuations of average transverse momentum in central Pb+Pb collisions at 158 GeV per nucleon
We present first data on event-by-event fluctuations in the average
transverse momentum of charged particles produced in Pb+Pb collisions at the
CERN SPS. This measurement provides previously unavailable information allowing
sensitive tests of microscopic and thermodynamic collision models and to search
for fluctuations expected to occur in the vicinity of the predicted QCD phase
transition. We find that the observed variance of the event-by-event average
transverse momentum is consistent with independent particle production modified
by the known two-particle correlations due to quantum statistics and final
state interactions and folded with the resolution of the NA49 apparatus. For
two specific models of non-statistical fluctuations in transverse momentum
limits are derived in terms of fluctuation amplitude. We show that a
significant part of the parameter space for a model of isospin fluctuations
predicted as a consequence of chiral symmetry restoration in a non-equilibrium
scenario is excluded by our measurement.Comment: 6 pages, 2 figures, submitted to Phys. Lett.
NA49 Results on Single Particle and Correlation Measurements in Central Pb+Pb Collisions
Single-particle spectra and two-particle correlation functions measured by the NA49 collaboration in central Pb+Pb collisions at 158 GeV/nucleon are presented. These measurements are used to study the kinetic and chemical freeze-out conditions in heavy ion collisions. We conclude that large baryon stopping, high baryon density and strong transverse radial flow are achieved in central Pb+Pb collisions at the SPS.Single-particle spectra and two-particle correlation functions measured by the NA49 collaboration in central Pb+Pb collisions at 158 GeV/nucleon are presented. These measurements are used to study the kinetic and chemical freeze-out conditions in heavy ion collisions. We conclude that large baryon stopping, high baryon density and strong transverse radial flow are achieved in central Pb+Pb collisions at the SPS
Xi and Xi-bar Production in 158 GeV/Nucleon Pb+Pb Collisions
We report measurements of Xi and Xi-bar hyperon absolute yields as a function
of rapidity in 158 GeV/c Pb+Pb collisions. At midrapidity, dN/dy = 2.29 +/-
0.12 for Xi, and 0.52 +/- 0.05 for Xi-bar, leading to the ratio of Xi-bar/Xi =
0.23 +/- 0.03. Inverse slope parameters fitted to the measured transverse mass
spectra are of the order of 300 MeV near mid-rapidity. The estimated total
yield of Xi particles in Pb+Pb central interactions amounts to 7.4 +/- 1.0 per
collision. Comparison to Xi production in properly scaled p+p reactions at the
same energy reveals a dramatic enhancement (about one order of magnitude) of Xi
production in Pb+Pb central collisions over elementary hadron interactions.Comment: 15 page
A joint-ParB interface promotes Smc DNA recruitment.
Chromosomes readily unlink and segregate to daughter cells during cell division, highlighting a remarkable ability of cells to organize long DNA molecules. SMC complexes promote DNA organization by loop extrusion. In most bacteria, chromosome folding initiates at dedicated start sites marked by the ParB/parS partition complexes. Whether SMC complexes recognize a specific DNA structure in the partition complex or a protein component is unclear. By replacing genes in Bacillus subtilis with orthologous sequences from Streptococcus pneumoniae, we show that the three subunits of the bacterial Smc complex together with the ParB protein form a functional module that can organize and segregate foreign chromosomes. Using chimeric proteins and chemical cross-linking, we find that ParB directly binds the Smc subunit. We map an interface to the Smc joint and the ParB CTP-binding domain. Structure prediction indicates how the ParB clamp presents DNA to the Smc complex, presumably to initiate DNA loop extrusion
Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.
Bacterial chromosomes are folded into tightly regulated three-dimensional structures to ensure proper transcription, replication, and segregation of the genetic information. Direct visualization of chromosomal shape within bacterial cells is hampered by cell-wall confinement and the optical diffraction limit. Here, we combine cell-shape manipulation strategies, high-resolution fluorescence microscopy techniques, and genetic engineering to visualize the shape of unconfined bacterial chromosome in real-time in live Bacillus subtilis cells that are expanded in volume. We show that the chromosomes predominantly exhibit crescent shapes with a non-uniform DNA density that is increased near the origin of replication (oriC). Additionally, we localized ParB and BsSMC proteins - the key drivers of chromosomal organization - along the contour of the crescent chromosome, showing the highest density near oriC. Opening of the BsSMC ring complex disrupted the crescent chromosome shape and instead yielded a torus shape. These findings help to understand the threedimensional organization of the chromosome and the main protein complexes that underlie its structure
Relief of ParB autoinhibition by parS DNA catalysis and recycling of ParB by CTP hydrolysis promote bacterial centromere assembly.
Three-component ParABS systems are widely distributed factors for plasmid partitioning and chromosome segregation in bacteria. ParB acts as adaptor protein between the 16-base pair centromeric parS DNA sequences and the DNA segregation proteins ParA and Smc (structural maintenance of chromosomes). Upon cytidine triphosphate (CTP) and parS DNA binding, ParB dimers form DNA clamps that spread onto parS-flanking DNA by sliding, thus assembling the so-called partition complex. We show here that CTP hydrolysis is essential for efficient chromosome segregation by ParABS but largely dispensable for Smc recruitment. Our results suggest that CTP hydrolysis contributes to partition complex assembly via two mechanisms. It promotes ParB unloading from DNA to limit the extent of ParB spreading, and it recycles off-target ParB clamps to allow for parS retargeting, together super-concentrating ParB near parS. We also propose a model for clamp closure involving a steric clash when binding ParB protomers to opposing parS half sites
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