35 research outputs found
Preliminary measurements of turbulence and environmental parameters in a sub-tropical estuary of Eastern Australia
In natural systems, mixing is driven by turbulence, but current knowledge is very limited in estuarine zones where predictions of contaminant dispersion are often inaccurate. A series of detailed field studies was conducted in a small subtropical creek in eastern Australia. Hydrodynamic, physio-chemical and ecological measurements were conducted simultaneously to assess the complexity of the estuarine zone and the interactions between turbulence and environment. The measurements were typically performed at high frequency over a tidal cycle. The results provide an original data set to complement long-term monitoring and the basis for a more detailed study of mixing in sub-tropical systems. Unlike many long-term observations, velocity and water quality scalars were measured herein with sufficient spatial and temporal resolutions to determine quantities of interest in the study of turbulence, while ecological indicators were sampled systematically and simultaneously. In particular the results yielded contrasted outcomes, and the finding impacts on the selection process for key water quality indicators
The Inflammatory Kinase MAP4K4 Promotes Reactivation of Kaposi's Sarcoma Herpesvirus and Enhances the Invasiveness of Infected Endothelial Cells
Kaposi's sarcoma (KS) is a mesenchymal tumour, which is caused by Kaposi's sarcoma herpesvirus (KSHV) and develops under inflammatory conditions. KSHV-infected endothelial spindle cells, the neoplastic cells in KS, show increased invasiveness, attributed to the elevated expression of metalloproteinases (MMPs) and cyclooxygenase-2 (COX-2). The majority of these spindle cells harbour latent KSHV genomes, while a minority undergoes lytic reactivation with subsequent production of new virions and viral or cellular chemo- and cytokines, which may promote tumour invasion and dissemination. In order to better understand KSHV pathogenesis, we investigated cellular mechanisms underlying the lytic reactivation of KSHV. Using a combination of small molecule library screening and siRNA silencing we found a STE20 kinase family member, MAP4K4, to be involved in KSHV reactivation from latency and to contribute to the invasive phenotype of KSHV-infected endothelial cells by regulating COX-2, MMP-7, and MMP-13 expression. This kinase is also highly expressed in KS spindle cells in vivo. These findings suggest that MAP4K4, a known mediator of inflammation, is involved in KS aetiology by regulating KSHV lytic reactivation, expression of MMPs and COX-2, and, thereby modulating invasiveness of KSHV-infected endothelial cells. © 2013 Haas et al
Actions régulatrices des cytokines dans des systèmes cellulaires impliqués dans les processus invasifs: étude de la balance Matrixines/Inhibiteurs tissulaires des métalloprotéinases dans les cellules endothéliales et tumorales, en particulier dans les cellules du sarcome de Kaposi
Doctorat en sciences médicalesinfo:eu-repo/semantics/nonPublishe
Actions régulatrices des cytokines dans des systèmes cellulaires impliqués dans les processus invasifs: étude de la balance Matrixines/Inhibiteurs tissulaires des métalloprotéinases dans les cellules endothéliales et tumorales, en particulier dans les cellules du sarcome de Kaposi
Doctorat en sciences médicalesinfo:eu-repo/semantics/nonPublishe
Inhibition of Cowdria ruminantium infectious yield by interferons alpha and gamma in endothelial cells.
We have shown before that there is a positive correlation between resistance of cattle against Cowdria infection and early IFN production. Our in vitro studies demonstrated an activity of rBoIFN alpha 2C and rBoIFN gamma against Cowdria in bovine endothelial cells of brain microvasculature (BMEC). rBoIFN gamma is much more active in this respect than rBoIFN alpha 2C. These results suggest a role of IFNs in the resistance against the disease. Strikingly, in the same conditions rBoIFN alpha 2C has no effect on the yield of Cowdria from infected bovine endothelial cells of umbilical artery origin (BUEC). Similarly we showed that HuIFNa had no effect on the multiplication of Cowdria in human vein umbilical endothelial cells (HUVEC). We found no differences in the capacity of BUE and BME cells to bind rBoIFN alpha 2C. This may reflect a true difference between capillary and large blood vessels.SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Redistribution of velocity and bed shear stress in straight and curved open channels by means of a bubble screen
Redistribution of velocity and bed shear stress in straight and curved open channels by means of a bubble screen
Inhibition of Cowdria ruminantium infectious yield by interferons alpha and gamma in endothelial cells.
We have shown before that there is a positive correlation between resistance of cattle against Cowdria infection and early IFN production. Our in vitro studies demonstrated an activity of rBoIFN alpha 2C and rBoIFN gamma against Cowdria in bovine endothelial cells of brain microvasculature (BMEC). rBoIFN gamma is much more active in this respect than rBoIFN alpha 2C. These results suggest a role of IFNs in the resistance against the disease. Strikingly, in the same conditions rBoIFN alpha 2C has no effect on the yield of Cowdria from infected bovine endothelial cells of umbilical artery origin (BUEC). Similarly we showed that HuIFNa had no effect on the multiplication of Cowdria in human vein umbilical endothelial cells (HUVEC). We found no differences in the capacity of BUE and BME cells to bind rBoIFN alpha 2C. This may reflect a true difference between capillary and large blood vessels.SCOPUS: ar.jinfo:eu-repo/semantics/publishe