Submillimeter-Wave Measurements and Analysis of the Ground and ν2 = 1 States of Water

In order to facilitate further studies of water in the interstellar medium, the envelopes of late-type stars, jets, and shocked regions, the frequencies of 17 newly measured H_2 ^(16)O transitions between 0.841 and 1.575 THz are reported. A complete update of the available water line frequencies and a detailed calculation of unmeasured rotational transitions and transition intensities as a function of temperature are presented for the ground and ν_2 = 1 state levels below 3000 cm^(-1) of excitation energy. The new terahertz transitions were measured with a recently developed laser difference frequency spectrometer. Six of these transitions arise from the ν_2 = 1 state, and the other 11 are in the ground state; all have lower state energies from 700 to 1750 cm^(-1) and should be accessible to Stratospheric Observatory For Infrared Astronomy (SOFIA) through the atmosphere. The transitions near 0.850 THz are accessible from the ground with existing receivers. Observations of the newly measured ν_2 = 1 state transitions, which include the 1_(1, 1)-0_(0, 0) fundamental at 1.2057 THz and five other very low J transitions, should provide valuable insights into role played by the ν2 = 1 state in the cooling dynamics of jets, shocks, masers, and strongly infrared-pumped regions. The line list is presented to assist in the planning of observational campaigns with the Far-Infrared Space Telescope (FIRST) and other proposed space missions with which a full suite of water observations can be carried out

A tunable cavity-locked diode laser source for terahertz photomixing

An all solid-state approach to the precise frequency synthesis and control of widely tunable terahertz radiation by differencing continuous-wave diode lasers at 850 nm is reported in this paper. The difference frequency is synthesized by three fiber-coupled external-cavity laser diodes. Two of the lasers are Pound-Drever-Hall locked to different orders of a Fabry-Perot (FP) cavity, and the third is offset-frequency locked to the second of the cavity-locked lasers using a tunable microwave oscillator. The first cavity-locked laser and the offset-locked laser produce the difference frequency, whose value is accurately determined by the sum of an integer multiple of the free spectral range of the FP cavity and the offset frequency. The dual-frequency 850-nm output of the three laser system is amplified to 500 mW through two-frequency injection seeding of a single semiconductor tapered optical amplifier. As proof of precision frequency synthesis and control of tunability, the difference frequency is converted into a terahertz wave by optical-heterodyne photomixing in low-temperature-grown GaAs and used for the spectroscopy of simple molecules. The 3-dB spectral power bandwidth of the terahertz radiation is routinely observed to be ≾1 MHz. A simple, but highly accurate, method of obtaining an absolute frequency calibration is proposed and an absolute calibration of 10^(-7) demonstrated using the known frequencies of carbon monoxide lines between 0.23-1.27 THz

A High Power Frequency-Stabilized Tunable Two-Frequency Diode Laser System for the Generation of Coherent THz-Waves by Photomixing

A tunable two-frequency high-power diode laser system at 850 nm for terahertz (Thz)-wave generation by photomixing in low-temperature-grown GaAs photo-conductors has been developed. The difference frequency is obtained through a three laser system, where two lasers are locked to different orders of a Fabry-Perot cavity and a third is offset-locked to the first, The difference-frequency signal is generated by the offset laser and the other cavity-locked laser. The spectral purity of the beat note is better than 1 MHz. The maximum output power of -500 mW was obtained by using the master oscillator power amplifier (MOPA) technique, simultaneous injection of two seed frequencies with a single semiconductor optical amplifier. Here we report the generation of THz waves and spectroscopy of acetonitrile as proof of concept

A study to compare two methods of near astigmatism testing

A study to compare two methods of near astigmatism testin

Two-frequency MOPA diode laser system for difference-frequency generation of coherent THz waves

We developed a tunable, cavity-locked diode laser source at 850 nm for difference-frequency generation of coherent THz- waves. The difference frequency is synthesized by three fiber-coupled external-cavity diode lasers, where tow of the lasers are locked to adjacent modes of an ultra-stable Fabry-Perot cavity and the third laser is offset-phase- locked to the second cavity-locked laser using a tunable microwave oscillator. The first cavity-locked laser and the offset-locked laser produces the difference frequency, whose value is precisely determined by sum of integer multiple of free spectral range of the Fabry-Perot cavity and the offset frequency. The difference-frequency signal is amplified to 500 mW by the master oscillator power amplifier technique, simultaneous two-frequency injection-seeding with a single semiconductor optical amplifier. Here we demonstrate the difference-frequency generation of THz waves with the low- temperature-grown GaAs photomixers and its application to high-resolution spectroscopy of simple molecules. An absolute frequency calibration was carried out with an accuracy of approximately 10^(-7) using CO lines in the THz region

Direct measurement of the fundamental rotational transitions of the OH radical by laser sideband spectroscopy

We report for the first time the direct (zero-field) spectra of the fundamental rotational transitions of the OH radical in its Ω = 3/2 and 1/2 states at 2509.9 and 1834.7 GHz using a recently developed far-infrared laser sideband spectrometer. These measurements have verified and refined the predictions of previous laser magnetic resonance (LMR) work, thereby confirming the far-infrared detection of interstellar OH. The increased accuracy of these direct measurements will be useful to future astronomical and atmospheric studies of these important transitions

A Case of Successful Treatment of Recurrent Urinary Tract infection By Extended-Spectrum Β-Lactamase Producing

Recurrent urinary tract infections (UTIs) are a challenging clinical entity that can be frustrating for patient and physician alike. Repeated rounds of antibiotics can select for multidrug-resistant organisms, further complicating care. We describe the successful use of fecal microbiota transplantation (FMT) for the treatment of recurrent extended-spectrum β-lactamase (ESBL)-producin

Emergence of Clinical Clostridioides Difficile Isolates With Decreased Susceptibility to Vancomycin

BACKGROUND: Clostridioides difficile infection (CDI) is a leading cause of hospital-associated antibiotic-related diarrhea and deaths worldwide. Vancomycin is one of the few antibiotics recommended for both nonsevere and severe CDI cases. We sought to determine whether vancomycin nonsusceptible C. difficile strains are circulating in the patient population. METHODS: Stool samples from patients with CDI were collected from 438 and 98 patients at a large university hospital in Houston, Texas, and Nairobi, Kenya, respectively. The stools were examined for the presence of vancomycin and metronidazole nonsusceptible C. difficile using broth dilution culture, Etest (BioMérieux, France), polymerase chain reaction (PCR), whole-genome sequencing, and in vivo testing in a CDI mouse model. RESULTS: Of the Houston stool samples, 114/438 (26%) had vancomycin nonsusceptible C. difficile isolates and 128/438 (29%) were metronidazole nonsusceptible. Similarly, 66 out of 98 (67%) and 83/98 (85%) of the Nairobi patients harbored vancomycin and metronidazole nonsusceptible isolates, respectively. Vancomycin treatment of a CDI mouse model infected with a vancomycin nonsusceptible isolate failed to eradicate the infection. Whole-genome sequencing analyses did not identify vanA genes, suggesting a different mechanism of resistance. CONCLUSIONS: C. difficile strains exhibiting reduced susceptibility to vancomycin are currently circulating in patient populations. The spread of strains resistance to vancomycin, a first-line antibiotic for CDI, poses a serious therapeutic challenge. Routine susceptibility testing may be necessary

Two-frequency MOPA diode laser system for difference-frequency generation of coherent THz waves

We developed a tunable, cavity-locked diode laser source at 850 nm for difference-frequency generation of coherent THz- waves. The difference frequency is synthesized by three fiber-coupled external-cavity diode lasers, where tow of the lasers are locked to adjacent modes of an ultra-stable Fabry-Perot cavity and the third laser is offset-phase- locked to the second cavity-locked laser using a tunable microwave oscillator. The first cavity-locked laser and the offset-locked laser produces the difference frequency, whose value is precisely determined by sum of integer multiple of free spectral range of the Fabry-Perot cavity and the offset frequency. The difference-frequency signal is amplified to 500 mW by the master oscillator power amplifier technique, simultaneous two-frequency injection-seeding with a single semiconductor optical amplifier. Here we demonstrate the difference-frequency generation of THz waves with the low- temperature-grown GaAs photomixers and its application to high-resolution spectroscopy of simple molecules. An absolute frequency calibration was carried out with an accuracy of approximately 10^(-7) using CO lines in the THz region