Influence of Beef Carcass Chilling Rate on Steak Case Life and Quality Traits

Objective To determine if beef carcass weight influences chilling rate, pH decline, beef color, case life and tenderness of steaks from the round, loin, rib and chuck

Temperature During Beef Product Transport Impacts Subprimal and Steak Yield

Objective The objective of this study was to determine the impact of ambient temperature during striploin and sirloin transport on moisture loss, color, and tenderness

Using Precision Technology to Measure Cattle Methane Emissions and Intake on Western South Dakota Rangelands

Study Description: Cattle DMI is an essential component of calculating cattle stocking rates, determining nutrient requirements, and evaluating feed efficiency. Cattle DMI and digestion of forages impact enteric greenhouse gas (GHG) emissions; a major public and environmental concern. Increased GHG levels indicate energy loss during the rumen fermentation process. Obtaining data for rangeland cattle DMI and GHG emissions is needed to understand and enhance individual animal performance and reduce negative environmental impacts. We will develop enteric emissions and DMI relationships by conducting three feeding trials using the GreenFeed and SmartFeed Pro (C-Lock Inc. Rapid City, SD). The GreenFeed will measure real-time gas fluxes and the SmartFeed Pro will measure daily intake by calculating disappearance from the feeder. The three feeding trials will consist of dry beef cows (n = 12) receiving low, high, and intermediate quality forages treatments with a 15 day adjustment period and a 15 day period of collection. Using these data, regression, artificial neural network, and dynamic-mechanistic models will develop and assessed to identify a model that accurately and precisely predicts forage DMI for dry beef cows on pasture

Influence of Beef Carcass Chilling Rate on Steak Case Life and Quality Traits

Study Description: Twelve head of fed beef cattle were harvested at the SDSU Meat Laboratory over two days. Carcasses were allotted into two weight groups based on hot carcass weight (HCW): Heavyweight (HW) and Lightweight (LW). Data logging thermometers were placed in the left side of each carcass within the round, loin, rib, and chuck primals to track temperature decline. Carcass measurements including 12th rib fat thickness, ribeye area and marbling score were collected approximately 48 hours postmortem. Steaks from each primal were collected to measure Warner-Bratzler shear force (WBSF), objective and subjective color

Influence of Carcass Chilling System on Chuck, Loin, and Round Temperature Decline, Carcass Characteristics, and Tenderness

Objective Determine the effect of chilling systems on beef carcass temperature decline, carcass characteristics, and tenderness

Beef Carcass Weight and Quality Grade Influence Tenderness

Objective The objective was to investigate whether hot carcass weight (HCW) affected Warner-Bratzler shear force (WBSF) and tenderness formation of Denver, strip, and eye of round steaks from USDA Select (Se) and low Choice (LC) beef carcasses.Study Description Select and LC carcasses were selected at a commercial beef plant by HCW (light = 650–750 lb; middle = 850–950 lb; heavy = 1,025–1,150 lb). Steaks were fabricated and aged for 5, 10, and 14 days. Tenderness was determined through WBSF based on industry standards. Additionally, protein degradation was analyzed to determine tenderness formation

Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein

AbstractHybridoma cell lines (2E8, 8G8 and 5G12) producing fully human monoclonal antibodies (hMAbs) specific for the pre-membrane (prM) protein of West Nile virus (WNV) were prepared using a human fusion partner cell line, MFP-2, and human peripheral blood lymphocytes from a blood donor diagnosed with WNV fever in 2004. Using site-directed mutagenesis of a WNV-like particle (VLP) we identified 4 amino acid residues in the prM protein unique to WNV and important in the binding of these hMAbs to the VLP. Residues V19 and L33 are important epitopes for the binding of all three hMAbs. Mutations at residue, T20 and T24 affected the binding of hMAbs, 8G8 and 5G12 only. These hMAbs did not significantly protect AG129 interferon-deficient mice or Swiss Webster outbred mice from WNV infection

Amygdala and Ventrolateral Prefrontal Cortex Function during Anticipated Peer Evaluation in Pediatric Social Anxiety

1. Context. Amygdala and ventrolateral prefrontal cortex dysfunction manifests in adolescents with anxiety disorders when they view negatively-valenced stimuli in threatening contexts. Such fear-circuitry dysfunction may also manifest when anticipated social evaluation leads socially anxious adolescents to misperceive peers as threatening. 2. Objective. To determine whether photographs of negatively-evaluated smiling peers, viewed during anticipated evaluation, engage the amygdala and ventrolateral prefrontal cortex differentially in adolescents with and without social anxiety. 3. Design. Case-control study. 4. Setting. Government clinical research institute. 5. Participants. Fourteen adolescents with anxiety disorders associated with marked social concerns and 14 diagnosis-free adolescents, matched on sex, age, IQ, and socio-economic status. 6. Main Outcome Measure(s). Blood oxygenation level-dependent signal measured with event-related functional magnetic resonance imaging. Before and during neuroimaging scans, participants anticipating social evaluation completed peer- and self-appraisals. Event-related analyses were tailored to participants’ ratings of specific peers. 7. Results. Participants classified 40 pictures of same-age peers as ones they wanted to engage or not engage with for a social interaction. Anxious adolescents showed greater amygdala activation than healthy adolescents when anticipating evaluation from peers rated as undesired for an interaction. Viewing undesired peers engaged stronger positive amygdala-ventrolateral-prefrontal-cortex connectivity in anxious vs. healthy adolescents. 8. Conclusions. Anticipating social evaluation from negatively-perceived peers modulates amygdala and ventrolateral prefrontal cortex engagement differentially in anxious and healthy 3 adolescents. Amygdala and ventrolateral prefrontal cortex abnormalities in adolescent anxiety disorders are heightened in specific contexts of potential peer evaluation