8 research outputs found
Process analytical utility of Raman spectroscopy for cell therapy manufacturing
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Process Analytical Utility of Raman Microspectroscopy in the Directed Differentiation of Human Pancreatic Insulin-Positive Cells
Continued
advances toward cell-based therapies for human disease
generate a growing need for unbiased and label-free monitoring of
cellular characteristics. We used Raman microspectroscopy to characterize
four important stages in the 26-day directed differentiation of human
embryonic stem cells (hESCs) to insulin-positive cells. The extent
to which the cells retained spectroscopic features of pluripotent
cells or developed spectroscopic features suggestive of pancreatic
endocrine cells, as well as assessing the homogeneity of the cell
populations at these developmental stages, were of particular interest.
Such information could have implications for the utility of Raman
microspectroscopy process analysis for the generation of insulin-positive
cells from hESCs. Because hESC seeding density influences the subsequent
pancreatic development, three different seeding density cultures were
analyzed. Transcription factor and other marker analyses assessed
the progress of the cells through the relevant developmental stages.
Increases in the Raman protein-to-nucleic acid band ratios were observed
at the final endocrine stage analyzed, but this increase was less
than expected. Also, high glycogen band intensities, somewhat unexpected
in pancreatic endocrine cells, suggested the presence of a substantial
number of glycogen containing cells. We discuss the potential process
analytical technology application of these findings and their importance
for cell manufacturing
Treatment of Dementia With Bosutinib: An Open-Label Study of a Tyrosine Kinase Inhibitor.
Objective: The pursuit of an effective therapeutic intervention for dementia has inspired interest in the class of medications known as tyrosine kinase inhibitors such as bosutinib.
Methods: Thirty-one patients with probable Alzheimer dementia or Parkinson spectrum disorder with dementia completed 12 months of bosutinib therapy and an additional 12 months of follow-up. The Clinical Dementia Rating scale (as estimated by the Quick Dementia Rating System [QDRS]) was the primary cognitive status outcome measure. Secondary outcome measures included the Repeatable Battery Assessment of Neuropsychological Status (RBANS) and the Montreal Cognitive Assessment. Cox regression methods were used to compare results with population-based estimates of cognitive decline.
Results: The present article reports on cognitive outcomes obtained at 12 months for 31 participants and up to 24 months for a 16-participant subset. Safety and tolerability of bosutinib were confirmed among the study population (M
Conclusions: Results support an overall positive outcome after 1 year of bosutinib. Future studies should explore the relationship between tyrosine kinases and neurodegenerative pathology as well as related avenues of treatment