On the biological and genetic diversity in Neospora caninum

Published: 22 March 2010Neospora caninum is a parasite regarded a major cause of foetal loss in cattle. A key requirement to an understanding of the epidemiology and pathogenicity of N. caninum is knowledge of the biological characteristics of the species and the genetic diversity within it. Due to the broad intermediate host range of the species, worldwide geographical distribution and its capacity for sexual reproduction, significant biological and genetic differences might be expected to exist. N. caninum has now been isolated from a variety of different host species including dogs and cattle. Although isolates of this parasite show only minor differences in ultrastructure, considerable differences have been reported in pathogenicity using mainly mouse models. At the DNA level, marked levels of polymorphism between isolates were detected in mini- and microsatellites found in the genome of N. caninum. Knowledge of what drives the biological differences that have been observed between the various isolates at the molecular level is crucial in aiding our understanding of the epidemiology of this parasite and, in turn, the development of efficacious strategies, such as live vaccines, for controlling its impact. The purpose of this review is to document and discuss for the first time, the nature of the diversity found within the species Neospora caninum.Sarwat E. Al-Qassab, Michael P. Reichel and John T. Elli

Operational modal analysis on a lighthouse structure subjected to ice actions

The sea ice interaction with a structure may cause system changes and affect the feasibility of common approaches to track modal parameters. In this paper, a covariance-driven stochastic subspace identification method is used to identify the natural frequencies from 190 time series of ice actions against a lighthouse structure. The results are sorted into groups defined by the observed ice conditions and governing ice failure mechanisms during the ice-structure interaction. The identified natural frequencies vary substantially within each individual group and between the groups. Recordings with flexural failures and a north-south ice-drift direction consistently rendered the same identified frequencies, whereas crushing seemed to create large amounts of variability in the identified frequencies. The results show the need for more high-fidelity data to assess whether modern system identification methods can be used to monitor system changes and support decision-making for operations of structures in ice-infested waters, such as offshore wind structures.</p