Avaliação da resposta pulpar a diferentes materiais odontológicos após capeamento direto : estudo ex vivo

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Ciências da Saúde, 2010.Para o procedimento de capeamento pulpar direto, a Odontologia utiliza materiais sintéticos com o objetivo de manter a vitalidade do tecido pulpar e substituir a estrutura mineralizada perdida por cárie ou trauma. O objetivo deste estudo foi avaliar as alterações ocorridas no tecido pulpar e a formação de estrutura mineralizada formada em resposta a diferentes materiais em dentes cultivados ex vivo. Dentes terceiros molares humanos (n=45) em fase de formação radicular, com o objetivo de comparar a resposta do tecido pulpar ao capeamento direto utilizando diferentes materiais odontológicos: agregado trióxido mineral, hidróxido de cálcio, cimento de óxido de zinco-eugenol e cimento de ionômero de vidro. As amostras foram cultivadas após exposição pulpar por 1, 14 e 28 dias e em seguida foram processadas para análise histológica. Tipo de inflamação e sua intensidade, hiperemia, necrose, camada de células odontoblásticas, continuidade e espessura da estrutura mineralizada formada foram analisados. Os grupos não se diferiram estatisticamente quanto aos aspectos da resposta pulpar avaliados, nem em relação a espessura da estrutura mineralizada formada. Porém os grupos tratados com hidróxido de cálcio e cimento de ionômero de vidro apresentaram formação de estrutura mineralizada completa após 14 dias, ao contrário dos outros materiais utilizados. _________________________________________________________________________________ ABSTRACTDirect pulp capping is the use of a biocompatible material on pulp that has been exposed during removal of caries or by traumatic injuries. This study was based on an ex vivo entire tooth culture model of human immature third molars. Direct pulp capping was made in 45 teeth third molars, using calcium hydroxide, mineral trioxide aggregate, zinc oxide eugenol cement and glass-ionomer cement. Then the pulp response and the mineralized tissue were analyzed, after 1, 14 and 28 days of culture, but in the parameters’ analyzed there weren´t difference between the odontologic materials

Comparative isolation protocols and characterization of stem cells from human primary and permanent teeth pulp

Aim: This study was developed to compare the morphological, proliferative and immunophenotypic profiles of pulp cells from permanent and primary teeth, obtained by two isolation methods. Methods: Normal human impacted third molars and exfoliated primary teeth were collected and cut around the cementoenamel junction. Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. Morphological and proliferative analyses, as well as immunophenotype characterization with monoclonal antibodies against CD117, CD34 and CD45 surface receptors were performed. Results: For the permanent teeth, on the 4th day of culture, the cell number was significantly higher for the outgrowth method. By the end of the studied period (14th day), the enzymatic method was more efficient in promoting culture growth. On the other hand, for primary teeth, enzymatic digestion always promoted a higher cell proliferation. The immunophenotypic profiles were CD117+/ CD34-/ CD45- and CD117+/ CD34+/ CD45- for cells from permanent and primary teeth, respectively. Conclusions: The findings of this study indicate that both isolation methods can be efficiently used. The cell population displayed an immunophenotype compatible to the one of stem cells, with remarkable positive expression of CD117

Comparative isolation protocols and characterization of stem cells from human primary and permanent teeth pulp

is study was developed to compare the morphological, proliferative and immunophenotypic profiles of pulp cells from permanent and primary teeth, obtained by two isolation methods. Methods: Normal human impacted third molars and exfoliated primary teeth were collected and cut around the cementoenamel junction. Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. Morphological and proliferative analyses, as well as immunophenotype characterization with monoclonal antibodies against CD117, CD34 and CD45 surface receptors were performed. Results: For the permanent teeth, on the 4th day of culture, the cell number was significantly higher for the outgrowth method. By the end of the studied period (14th day), the enzymatic method was more efficient in promoting culture growth. On the other hand, for primary teeth, enzymatic digestion always promoted a higher cell proliferation. The immunophenotypic profiles were CD117+/ CD34-/ CD45- and CD117+/ CD34+/ CD45- for cells from permanent and primary teeth, respectively. Conclusions: The findings of this study indicate that both isolation methods can be efficiently used. The cell population displayed an immunophenotype compatible to the one of stem cells, with remarkable positive expression of CD117