Thujone-Rich Fraction of Thuja occidentalis Demonstrates Major Anti-Cancer Potentials: Evidences from In Vitro Studies on A375 Cells

Crude ethanolic extract of Thuja occidentalis (Fam: Cupressaceae) is used as homeopathic mother tincture (TOΦ) to treat various ailments, particularly moles and tumors, and also used in various other systems of traditional medicine. Anti-proliferative and apoptosis-inducing properties of TOΦ and the thujone-rich fraction (TRF) separated from it have been evaluated for their possible anti-cancer potentials in the malignant melanoma cell line A375. On initial trial by S-diphenyltetrazolium bromide assay, both TOΦ and TRF showed maximum cytotoxic effect on A375 cell line while the other three principal fractions separated by chromatography had negligible or no such effect, because of which only TRF was further characterized and subjected to certain other assays for determining its precise anti-proliferative and apoptotic potentials. TRF was reported to have a molecular formula of C10H16O with a molecular weight of 152. Exposure of TRF of Thuja occidentalis to A375 cells in vitro showed more cytotoxic, anti-proliferative and apoptotic effects as compared with TOΦ, but had minimal growth inhibitory responses when exposed to normal cells (peripheral blood mononuclear cell). Furthermore, both TOΦ and TRF also caused a significant decrease in cell viability, induced inter-nucleosomal DNA fragmentation, mitochondrial transmembrane potential collapse, increase in ROS generation, and release of cytochrome c and caspase-3 activation, all of which are closely related to the induction of apoptosis in A375 cells. Thus, TRF showed and matched all the anti-cancer responses of TOΦ and could be the main bio-active fraction. The use of TOΦ in traditional medicines against tumors has, therefore, a scientific basis

Single-Loop Multi-Objective Reliability-Based Design Optimization Using Chaos Control Theory and Shifting Vector with Differential Evolution

Multi-objective reliability-based design optimization (MORBDO) is an efficient tool for generating reliable Pareto-optimal (PO) solutions. However, generating such PO solutions requires many function evaluations for reliability analysis, thereby increasing the computational cost. In this paper, a single-loop multi-objective reliability-based design optimization formulation is proposed that approximates reliability analysis using Karush-Kuhn Tucker (KKT) optimality conditions. Further, chaos control theory is used for updating the point that is estimated through KKT conditions for avoiding any convergence issues. In order to generate the reliable point in the feasible region, the proposed formulation also incorporates the shifting vector approach. The proposed MORBDO formulation is solved using differential evolution (DE) that uses a heuristic convergence parameter based on hypervolume indicator for performing different mutation operators. DE incorporating the proposed formulation is tested on two mathematical and one engineering examples. The results demonstrate the generation of a better set of reliable PO solutions using the proposed method over the double-loop variant of multi-objective DE. Moreover, the proposed method requires 6×–377× less functional evaluations than the double-loop-based DE

Single-Loop Multi-Objective Reliability-Based Design Optimization Using Chaos Control Theory and Shifting Vector with Differential Evolution

Multi-objective reliability-based design optimization (MORBDO) is an efficient tool for generating reliable Pareto-optimal (PO) solutions. However, generating such PO solutions requires many function evaluations for reliability analysis, thereby increasing the computational cost. In this paper, a single-loop multi-objective reliability-based design optimization formulation is proposed that approximates reliability analysis using Karush-Kuhn Tucker (KKT) optimality conditions. Further, chaos control theory is used for updating the point that is estimated through KKT conditions for avoiding any convergence issues. In order to generate the reliable point in the feasible region, the proposed formulation also incorporates the shifting vector approach. The proposed MORBDO formulation is solved using differential evolution (DE) that uses a heuristic convergence parameter based on hypervolume indicator for performing different mutation operators. DE incorporating the proposed formulation is tested on two mathematical and one engineering examples. The results demonstrate the generation of a better set of reliable PO solutions using the proposed method over the double-loop variant of multi-objective DE. Moreover, the proposed method requires 6×–377× less functional evaluations than the double-loop-based DE

Prevalent Cattle diseases of West Bengal: A survey in four districts

West Bengal is one of the most populated states of India. The residents of the state are mostly dependent on agriculture and allied activities for their livelihood. Livestock also provide economic support and food security to them. A survey in veterinary hospitals and cattle farms of Murshidabad, Nadia, Malda and Birbhum district of West Bengal indicated the frequent occurrence of bacterial, fungal, viral and protozoan diseases in cattle. Some of these diseases like cryptosporidium diarrhoea, ringworm and salmonelliasis are zoonotic in nature and thus, are easily transmitted to human. The frequency of diseases increases during rainy season. Infection of alimentary canal and pneumonia are more common in calves whereas, arthritis is more common in male. Most of the common diseases of cattle like anthrax, rabies, foot and mouth disease, bovine tuberculosis, etc., can be prevented by proper vaccination. Some of these diseases can easily be diagnosed by modern techniques like ELISA, PCR, etc. But lack of veterinary hospitals, socio-economic backwardness and misconception have prevented easy diagnosis and treatment of these common diseases. Common antibiotic like kanamycin, antifungal drugs and antitoxins are generally applied to treat these diseases. Further, maintenance of proper hygiene, isolation of diseased animal, regular check-up and vaccination can prevent easy transmission of the diseases and thus, will put a check on livestock damage