Quantification of yield gaps in rain-fed rice, wheat, cotton and mustard in India

Rainfed farming / Crop yield / Simulation / Rice / Wheat / Cotton / Mustard / India

Structure of human methaemoglobin: the variation of a theme

There has been considerable interest in the variability of the structure of the liganded haemoglobin after characterization of the R2 state in addition to the original relaxed R state. The structures of three crystallographically independent relaxed haemoglobin molecules have been determined through the X-ray structure analysis of the crystals of human methaemoglobin. The three molecules have quaternary structures intermediate between those of the R and R2 structures. The same is true about the disposition of residues in the 'switch' region. Thus it would appear that haemoglobin can access different relaxed states with varying degrees of similarity among them

eta_c production at the Large Hadron Collider

We have studied the production of the 1S_0 charmonium state, eta_c, at the Large Hadron Collider (LHC) in the framework of Non-Relativistic Quantum Chromodynamics (NRQCD) using heavy-quark symmetry. We find that NRQCD predicts a large production cross-section for this resonance at the LHC even after taking account the small branching ratio of eta_c into two photons. We show that it will be possible to test NRQCD through its predictions for eta_c, with the statistics that will be achieved at the early stage of the LHC, running at a center of mass energy of 7 TeV with an integrated luminosity of 100 pb^{-1}Comment: 8 pages, 2 figure

Heterologous expression of Arabidopsis laccase2, laccase4 and peroxidase52 driven under developing xylem specific promoter DX15 improves saccharification in populus

Background: Secondary cell wall holds considerable potential as it has gained immense momentum to replace the lignocellulosic feedstock into fuels. Lignin one of the components of secondary cell wall tightly holds the polysaccharides thereby enhancing the recalcitrance and complexity in the biomass. Laccases (LAC) and peroxidases (PRX) are the major phenyl-oxidases playing key functions during the polymerization of monolignols into lignin. Yet, the functions of laccase and peroxidases gene families remained largely unknown. Hence, the objective of this conducted study is to understand the role of specific LAC and PRX in Populus wood formation and to further investigate how the altered Lac and Prx expression affects biomass recalcitrance and plant growth. This study of heterologous expression of Arabidopsis Lac and Prx genes was conducted in poplar to avoid any otherwise occurring co-suppression mechanism during the homologous overexpression of highly expressed native genes. In the pursuit of optimizing lignocellulosic biomass for biofuel production, the present study focuses on harnessing the enzymatic potential of Arabidopsis thaliana Laccase2, Laccase4, and Peroxidase52 through heterologous expression. Results: We overexpressed selected Arabidopsis laccase2 (AtLac2), laccase4 (AtLac4), and peroxidase52 (AtPrx52) genes, based on their high transcript expression respective to the differentiating xylem tissues in the stem, in hybrid poplar (cv. 717) expressed under the developing xylem tissue-specific promoter, DX15 characterized the transgenic populus for the investigation of growth phenotypes and recalcitrance efficiency. Bioinformatics analyses conducted on AtLac2 and AtLac4 and AtPrx52, revealed the evolutionary relationship between the laccase gene and peroxidase gene homologs, respectively. Transgenic poplar plant lines overexpressing the AtLac2 gene (AtLac2-OE) showed an increase in plant height without a change in biomass yield as compared to the controls; whereas, AtLac4-OE and AtPrx52-OE transgenic lines did not show any such observable growth phenotypes compared to their respective controls. The changes in the levels of lignin content and S/G ratios in the transgenic poplar resulted in a significant increase in the saccharification efficiency as compared to the control plants. Conclusions: Overall, saccharification efficiency was increased by 35–50%, 21–42%, and 8–39% in AtLac2-OE, AtLac4-OE, and AtPrx52-OE transgenic poplar lines, respectively, as compared to their controls. Moreover, the bioengineered plants maintained normal growth and development, underscoring the feasibility of this approach for biomass improvement without compromising overall plant fitness. This study also sheds light on the potential of exploiting regulatory elements of DX15 to drive targeted expression of lignin-modifying enzymes, thereby providing a promising avenue for tailoring biomass for improved biofuel production. These findings contribute to the growing body of knowledge in synthetic biology and plant biotechnology, offering a sustainable solution to address the challenges associated with lignocellulosic biomass recalcitrance

Learning from M/EEG data with variable brain activation delays

International audienceMagneto- and electroencephalography (M/EEG) measure the electromagnetic signals produced by brain activity. In order to address the issue of limited signal-to-noise ratio (SNR) with raw data, acquisitions consist of multiple repetitions of the same experiment. An important challenge arising from such data is the variability of brain activations over the repetitions. It hinders statistical analysis such as prediction performance in a supervised learning setup. One such confounding variability is the time offset of the peak of the activation, which varies across repetitions. We propose to address this misalignment issue by explicitly modeling time shifts of different brain responses in a classification setup. To this end, we use the latent support vector machine (LSVM) formulation, where the latent shifts are inferred while learning the classifier parameters. The inferred shifts are further used to improve the SNR of the M/EEG data, and to infer the chronometry and the sequence of activations across the brain regions that are involved in the experimental task. Results are validated on a long term memory retrieval task, showing significant improvement using the proposed latent discriminative method

Palaeomagnetic, geochronological and geochemical study of Mesoproterozoic Lakhna Dykes in the Bastar Craton, India: Implications for the Mesoproterozoic supercontinent

Palaeomagnetic analysis of the Lakhna Dykes (Bastar Craton, India) yields a palaeopole at 36.6°N, 132.8°E, dp=12.4°, dm=15.9°, and the U--Pb zircon age obtained from one of the rhyolitic dykes is 1466.4±2.6 Ma (MSWD=0.21, concordia age based on two analyses with identical Pb--U ages), similar to previously published U\Pb ages. Major and trace element analyses of the Lakhna Dykes show shoshonitic and high-K calc-alkaline affinities consistent with a subduction related characteristics suggesting an active continental margin setting. This is in keeping with the Palaeo- to Mesoproterozoic tectonic environments in the eastern Indian margin. The new 1460 Ma Indian palaeopole was used to test possible palaeopositions of India within the Mesoproterozoic supercontinent Columbia. Of the four palaeomagnetically permissible reconstructions, juxtaposing western India against south-west Baltica is geologically the most reliably constrained and best fitting model. Our preferred reconstruction implies a long Palaeo- to Mesoproterozoic accretionary orogen stretching from south-eastern Laurentia through south-western Baltica to south-eastern India. Breakup of India and Baltica probably occurred in the Late Mesoproterozoic, but additional constraints are needed