Primeros registros documentados de la mojarra Cheirodon ibicuhiensis Eigenmann, 1915 (Characiformes, Cheirodontinae) para la Provincia de Córdoba, Argentina

In this work, the presence of Cheirodon ibicuhiensis is reported for the first time in the Primero River basin (Suquía) in the province of Córdoba, Argentina. This species had been previously reported in Argentina for the median and lower sections of the Uruguay and Paraná rivers. However, these new records of C. ibicuhiensis for the province of Córdoba extend its distribution to other basins outside of the east region of Argentina. The species have been observed in the Sierras Chicas region in the last decade, in four rivers: Chorrillos, Toro Muerto, Yuspe and Suquía. Morphometric measurements of nine specimens of this species and environmental parameters of the habitat where it was collected are also provided. Probably, C. ibicuhiensis was introduced into this river in order to have specimens for live-bait for fishing. It has always been observed forming shoals with other native mojarras of the province.En este trabajo, se reporta por primera vez la presencia de la mojarra Cheirodon ibicuhiensis para la cuenca del río Primero (Suquía) en la provincia de Córdoba, Argentina. Esta especie había sido citada en Argentina para las secciones media e inferior de los ríos Uruguay y Paraná. Estos registros de C. ibicuhiensis en la provincia de Córdoba amplían su distribución a otras cuencas no pertenecientes a la cuenca del Plata en Argentina. Los ejemplares fueron observados en la región de las Sierras Chicas de Córdoba, en los últimos diez años, en cuatro ríos serranos: Chorrillos, Toro Muerto, Yuspe y Suquía. Se exponen las medidas morfométricas de nueve ejemplares de esta mojarra y los parámetros ambientales del hábitat donde fue registrada. Esta especie habría sido introducida a los ríos serranos de la Córdoba con el fin de mantener ejemplares vivos para ser utilizados como carnada. Siempre se la ha observado formando cardúmenes con otras mojarras autóctonas de la provincia. Puntos destacados: Ampliación de la distribución de C. ibicuhiensis para Argentina. Primeros registros de C. ibicuhiensis en la provincia de Córdoba

First documented records of Cheirodon ibicuhiensis Eigenmann, 1915 (Characiformes, Cheirodontinae) for Córdoba province, Argentina

En este trabajo, se reporta por primera vez la presencia de la mojarra Cheirodon ibicuhiensis para la cuenca del río Primero (Suquía) en la provincia de Córdoba, Argentina. Esta especie había sido citada en Argentina para las secciones media e inferior de los ríos Uruguay y Paraná. Estos registros de C. ibicuhiensis en la provincia de Córdoba amplían su distribución a otras cuencas no pertenecientes a la cuenca del Plata en Argentina. Los ejemplares fueron observados en la región de las Sierras Chicas de Córdoba, en los últimos diez años, en cuatro ríos serranos: Chorrillos, Toro Muerto, Yuspe y Suquía. Se exponen las medidas morfométricas de nueve ejemplares de esta mojarra y los parámetros ambientales del hábitat donde fue registrada. Esta especie habría sido introducida a los ríos serranos de la Córdoba con el fin de mantener ejemplares vivos para ser utilizados como carnada. Siempre se la ha observado formando cardúmenes con otras mojarras autóctonas de la provincia.In this work, the presence of Cheirodon ibicuhiensis is reported for the first time in the Primero River basin (Suquía) in the province of Córdoba, Argentina. This species had been previously reported in Argentina for the median and lower sections of the Uruguay and Paraná rivers. However, these new records of C. ibicuhiensis for the province of Córdoba extend its distribution to other basins outside of the east region of Argentina. The species have been observed in the Sierras Chicas region in the last decade, in four rivers: Chorrillos, Toro Muerto, Yuspe and Suquía. Morphometric measurements of nine specimens of this species and environmental parameters of the habitat where it was collected are also provided. Probably, C. ibicuhiensis was introduced into this river in order to have specimens for live-bait for fishing. It has always been observed forming shoals with other native mojarras of the province.Fil: Rivetti, Natalia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Hued, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Bonifacio, Alejo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin

Composition And Structure Of A Mountain River Fish Community Along An Elevation Gradient

Volume: 87Start Page: 171End Page: 18

Trace element concentrations in six fish species from freshwater lentic environments and evaluation of possible health risks according to international standards of consumption

Multi-element concentrations (Al, As, Cd, Cr, Hg, Ni, Pb, Se, and Sr) were analyzed in the muscle of six fish species (Hoplias malabaricus, Oligosarcus jenynsii, Rhamdia quelen, Bryconamericus iheringii, Astyanax fasciatus, and Odontesthes bonariensis) with different diets and habits from the Río Tercero Reservoir (RTR) in Córdoba, Argentina, during the wet and dry seasons. Besides, potential human health risks, associated with the consumption of these elements, have been assessed considering the average daily intake (EDI) in children and adults. Additionally, the target hazard quotient (THQ) and carcinogenic risk (CR) were evaluated taking into account the intake by the general population, fishermen, and consumption frequency recommended by the American Heart Association (AHA), the maximum scientific reference in cardiology in the USA and worldwide. All species presented quantifiable values in muscle for all the analyzed elements (Al, As, Cr, Hg, Ni, Se, and Sr), except for Cd and Pb, being Al and Sr the most accumulated elements in all species in both seasons. The consumption of edible muscles of the species studied in this reservoir represents a toxicological risk to humans. Mercury and As were the main elements that presented a health risk through the consumption of fish. Their concentrations in most fish species were above the maximum daily allowable concentrations, and THQ values were several times greater than 1. In addition, according to AHA recommendations, the cancer risk caused by As was greater than the acceptable value of 10−4 in all species studied, and in both seasons, with the exception of A. fasciatus, in the rainy season. These results indicate that the consumption of fish from the RTR exposes the inhabitants to possible health risks, especially when considering the consumption frequency recommended by the AHA. Therefore, fish intake from this reservoir should be limited to minimize potential risks to the health of consumers. Finally, the results of this study are useful for controlling pollution and developing preventive and palliative policies to protect populations in contact not only with the reservoir but also with other areas of the world with similar conditions.Fil: Garnero, Paola Lorena. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Bistoni, Maria de Los Angeles. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentin

Uptake, tissue distribution and elimination in a native fish species Astyanax eigenmanniorum exposed to inorganic mercury

The increasing contamination of water bodies with mercury (Hg) raises concerns about the possible effects of this metal on native fish species. Our current understanding of its dynamics in fish organs remains limited. In this study, adult individuals of the native species Astyanax eigenmanniorum were exposed to three environmentally relevant HgCl2 concentrations (5, 100, and 170 μg L−1) for 96 h. To evaluate total Hg (THg) elimination, new individuals were exposed to 100 μg L−1 of HgCl2 (96 h), and at the end of the exposure period, half of the fish were placed in tanks with clean water for 168 h. In both assays, the organs were removed, and THg levels were measured using ICP-MS. The uptake of IHg in A. eigenmanniorum showed a differential accumulation in the organs. Gills, intestine, and brain were the tissues with the highest THg levels. Finally, no elimination of THg in the water was observed, but intestine and gills significantly removed the THg accumulated. Probably a Hg redistribution through the tissues could take place.Fil: Garnero, Paola Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin

Assessment of exposure to metals, As and Se in water and sediment of a freshwater reservoir and their bioaccumulation in fish species of different feeding and habitat preferences

The concentrations of metals (Ag, Al, Ba, B, Be, Bi, Cd, Co, Cr, Cu, Fe, Ga, Hg, Li, Mn, Mo, Ni, Pb, Rb, Sr, Tl, U, V, Zn), As and Se were analyzed in water and sediments from three sites of Río Tercero Reservoir (Córdoba, Argentina) during the wet and the dry season. The dynamics of metals in six fish species (Hoplias malabaricus, Oligosarcus jenynsii, Rhamdia quelen, Bryconamericus iheringii, Astyanax fasciatus and Odontesthes bonariensis) from the reservoir were investigated to discover the possible differential influence of habitat and diet on metal accumulation in the fish. In the abiotic matrix, the highest heavy metal concentrations were observed in sediment. The concentrations of Al, Cu and Pb in water exceeded the limits considered as hazardous for aquatic life. Potential ecological risk analysis of metal concentrations in sediment indicated a low ecological risk in Río Tercero Reservoir in all sampling periods. The enrichment factor indicated that Cu, Pb, Zn and Hg come from anthropogenic sources. Among five different organs, the highest metal levels were found in gills and intestine. Rhamdia quelen and Oligosarcus jenynsii were the species with the highest values of metal accumulation in the whole body. Our study showed that the accumulation pattern of these multi-elements in the different fish species did not respond to diet or habitat, but seemed to be related to the detoxification mechanisms and the metabolism of each organism.Fil: Garnero, Paola Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: González, Germán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Griboff, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin

Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality

Cytochrome P450 aromatase is the steroidogenic enzyme that catalyzes the conversion of androgens to estrogens. Teleosts express two structurally and functionally different P450 aromatase isoforms, termed Cytochrome 19a1a and Cytochrome 19a1b. The first is preferentially expressed in ovary while the second is constitutively expressed in brain. The aim of the present study was to evaluate changes in cyp19a1b expression in brain of Jenynsia multidentata collected in Suquía river (Córdoba, Argentina) in order to assess if this biological response reflects changes in water quality. For this purpose we first identified cyp19a1b parcial cDNA sequence and adjusted the method to quantify mRNA expression by qRT-PCR (quantitative RT-PCR). Then, water and fish samples were collected in five monitoring stations located along a pollution gradient in Suquía river. The qRT-PCR analysis of female brains reveals that cyp19a1b mRNA levels change as water pollution does, showing the highest levels at both unpolluted and recovered areas, with suppression as pollution increases. Even when cyp19a1b expression in J. multidentata will need further studies, the results here presented indicate that this biological response appear as a promising biomarker of water pollution that may also point out the probable presence of endocrine disruptors.Fil: Guyón, Noelia Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Amé, María Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentin

Potential human health risks from metals and As via Odontesthes bonariensis consumption and ecological risk assessments in a eutrophic lake

The concentration of Al, Cr, Fe, Mn, Ni, Cu, Zn, Hg, Sr, Mo, Ag, Cd, Pb and As was analyzed in water, sediment, and muscle of Odontesthes bonariensis from the eutrophic San Roque Lake (Córdoba-Argentina). The monitoring campaign was performed during the wet, dry and intermediate season. The concentration of Cr, Fe, Pb, Zn, Al and Cd in water exceeded the limits considered as hazardous for aquatic life. The highest metal concentrations were observed in sediment, intermediate concentrations, in fish muscle, and the lowest in water, with the exception of Cr, Zn, As and Hg, which were the highest in fish muscle. Potential ecological risk analysis of heavy metal concentrations in sediment indicated that the San Roque Lake posed a low ecological risk in all sampling periods. The target hazard quotients (THQs) and carcinogenic risk (CR) for individual metals showed that As in muscle was particularly hazardous, posing a potential risk for fishermen and the general population during all sampling periods. Hg poses a potential risk for fishermen only in the intermediate season. It is important to highlight that none of these two elements exceeded the limits considered as hazardous for aquatic life in water and sediment. This result proves the importance of performing measurements of contaminants, in both abiotic and biotic compartments, to assess the quality of food resources. These results suggest that the consumption of this fish species from this reservoir is not completely safe for human health.Fil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Garnero, Paola Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin

Effects of carbamazepine on cortisol levels and behavioral responses to stress in the fish Jenynsia multidentata

Carbamazepine (CBZ) is an anticonvulsant drug, prescribed worldwide for the treatment of epilepsy, bipolardisorder and trigeminal neuralgia, which has been frequently detected in aquatic environments. The objectiveof this study was to analyze if CBZ modifies scototaxis and shoaling behaviors and/or whole-body cortisol levelsof the one-sided livebearing fish Jenynsia multidentata under stress condition. Female adults of J. multidentatawere exposed to 0, 10, 50 and 200 μg CBZ/L during 14 days. After CBZ exposure, fish were subjected to restraintstress during 15 min. Control animals were not exposed to CBZ or stress. In the light/dark preference test(scototaxis), the individuals under acute restraint stress (without CBZ) exhibited a significant increase in themean speed and in the time spent both in the light compartment and in the bottom of the tank with respect tocontrols. They also showed a tendency to stay longer frozen in the light compartment. Fish exposed to 10 and50 μg CBZ/L showed a significant reduction in mean speed compared to stressed fish without CBZ. A reductionin the time spent in the bottom of the tank was also observed in fish exposed to 10 μg CBZ/L. Fish exposed to200 μg CBZ/L showed a decreasing tendency in all behavioral endpoints (time spent in the light compartment,mean speed, time spent at the bottom and freezing) in comparison to stressed fish not exposed to CBZ. Consideringwhole-body cortisol results, fish under acute restraint stress (without CBZ) significantly increased theirhormone levelswith respect to the control group,while fish exposed to CBZ and acute restraint stress, significantlydecreased their whole-body cortisol levels. There were no significant changes in shoaling behavior due toeither stress or CBZ exposure and no significant differences in whole-body cortisol levels between experimentalgroups. Considering that the light/dark and shoaling tests measure different stress response behaviors regulatedby different neuroendocrine systems, these results could indicate that CBZ has a differential effect on fish behavioralstress response and cortisol levels, depending on the behavioral test used and stressor applied.Fil: Calcagno, Emilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Durando, Patricia Evelina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Valdés, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Franchioni, Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin

Evaluation of the health status of the Silverside (Odontesthes bonariensis) at a RAMSAR Site in South America

The objective of this work was to evaluate the health status of an economic and ecologically important fish species from Mar Chiquita Lake, a RAMSAR site located in Cordoba, Argentina, relative to the levels of selected persistent organic pollutants (POPs) in lake water and fish tissues. Odontesthes bonariensis was used as a model species, and its health was estimated by means of histological indices in gills and liver. Sampling was performed according to rainy and dry seasons (i.e. dry, rainy and post-rainy). Gill and liver histopathology were evaluated by semi-quantitative indices and morphometric analysis. Although epithelial lifting in gills and lipid degeneration in liver were frequently registered, they are considered as reversible if environmental conditions improve. During rainy and post-rainy seasons fish presented significantly higher scores of liver and total indices. These higher index scores were correlated with increased levels of POPs in gill and liver tissue. Therefore, preventive measures are needed to mitigate the entry of these compounds into the lake.Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Hued, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Gonzalez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Miglioranza, Karina Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin