27 research outputs found
Cold-preservation of human adult hepatocytes for liver cell therapy
Hepatocyte transplantation is a promising alternative therapy for the treatment of hepatic failure, hepatocellular deficiency, and genetic metabolic disorders. Hypothermic preservation of isolated human hepatocytes is potentially a simple and convenient strategy to provide on-demand hepatocytes in sufficient quantity and of the quality required for biotherapy. In this study, first we assessed how cold storage in three clinically safe preservative solutions (UW, HTS-FRS, and IGL-1) affects the viability and in vitro functionality of human hepatocytes. Then we evaluated whether such cold-preserved human hepatocytes could engraft and repopulate damaged livers in a mouse model of liver failure. Human hepatocytes showed comparable viabilities after cold preservation in the three solutions. The ability of fresh and cold-stored hepatocytes to attach to a collagen substratum and to synthesize and secrete albumin, coagulation factor VII, and urea in the medium after 3 days in culture was also equally preserved. Cold-stored hepatocytes were then transplanted in the spleen of immunodeficient mice previously infected with adenoviruses containing a thymidine kinase construct and treated with a single dose of ganciclovir to induce liver injury. Engraftment and liver repopulation were monitored over time by measuring the blood level of human albumin and by assessing the expression of specific human hepatic mRNAs and proteins in the recipient livers by RT-PCR and immunohistochemistry, respectively. Our findings show that cold-stored human hepatocytes in IGL-1 and HTS-FRS preservative solutions can survive, engraft, and proliferate in a damaged mouse liver. These results demonstrate the usefulness of human hepatocyte hypothermic preservation for cell transplantation
Modulation de la réponse hémostatique (régulation de la synthèse et mécanismes inhibiteurs)
MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF
Syndrome des anticorps antiphospholipides (place des anticorps antiphosphatidylsérine dans les complications thrombotiques)
MONTPELLIER-BU Médecine (341722104) / SudocMONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
Intérêt du temps d'occlusion en situation obstétricale
MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF
Impact de la contraception orale et de la thrombophilie sur la maladie thromboembolique veineuse de la femme (Á propos de 123 patientes recrutées au CHU de Montpellier)
MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF
Prévalence des anomalies prothombotiques de l'hémostase dans les défauts d'implantation embryonnaire après fécondation In Vitro (comparaison avec les fausses couches spontanées à répétition et les pathologies vasculaires placentaires graves)
MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF
Mise en évidence des lupus anticoagulants B2GPI-dépendants par une nouvelle méthode et évaluation du risque de complications cliniques chez des patients atteints de syndrôme des anti-phospholipides
MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF
Prévalence des anomalies prothrombotiques de l'hémostase dans la thrombose veineuse mésentérique (comparaison avec la thrombose veineuse profonde des membres inférieurs)
MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF
Cultures primaires hépatocytaires en vue de transplantation hépatique (étude des facteurs V et VII dans le surnageant - influence de la conservation à froid)
MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF
Un modèle de culture primaire d'hépatocytes humains à long terme (évaluation des principales protéines de l'hémostase)
MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF