Učinak vrućice uzrokovane endotoksinom bakterije E. coli na farmakokinetiku i doziranje ceftriaksona u ovaca (Ovis aries).

The effect of fever was studied on the pharmacokinetics of ceftriaxone (CTRX) following intravenous administration at the rate of 50 mg/kg b.m. in Chhotanagpuri sheep. Fever was induced by intravenous administration of E. coli serovar O126:B8 lipopolysaccharide, and plasma CTRX concentration was estimated by HPLC. The plasma drug concentration versus time curve best fitted a two compartment open model. The maximum plasma drug concentration was 16.33 per cent lower in febrile sheep (FS) in comparison to normal sheep (NS). In FS, t1/2α was lower, while t1/2β was higher than in NS. Vdarea and P: C ratio was higher in FS, but ClB and AUC did not differ significantly between the two groups. The results of the present study indicate that CTRX has greater distribution in the peripheral compartment during pyrexia. High values of K12/K /K21 ratio also indicated rapid drug distribution in various body fluids and compartments. The suitable dosage regimen of CTRX in FS by the intravenous route was calculated to be 15 mg/kg b.m. at a 5 h interval.Istraživan je učinak vrućice na farmakokinetiku ceftriaksona (CTRX) nakon njegove intravenske primjene u dozi od 50 mg/kg tjelesne mase u ovaca pasmine Chhotanagpuri. Vrućica je bila uzrokovana intravenskom primjenom lipopolisaharida serovara O126:B8 bakterije E. coli. Koncentracija cefriaksona u plazmi bila je određivana visokotlačnom tekućinskom kromatografijom. Koncentracija lijeka u plazmi u odnosu na vremensku krivulju bila je najsličnija modelu dvaju odjeljaka. Najveća koncentracija lijeka bila je 16,33% manja u febrilnih ovaca u odnosu na nefebrilne. U febrilnih je ovaca t1/2α bio manji, a t1/2β veći nego u nefebrilnih. Prividni volumen raspodjele (Vdarea) i omjer P:C bili su veći u febrilnih ovaca dok se ClB (ukupni klirens lijeka iz organizma) i AUC (površina ispod koncentracijske krivulje lijeka u plazmi) nisu značajno razlikovali između dviju skupina. Rezultati ovog istraživanja naznačuju da ceftriakson ima bolju raspodjelu u perifernom odjeljku za vrijeme vrućice. Velike vrijednosti odnosa K12/K21 također upućuju na brzu raspodjelu lijeka u različitim tjelesnim tekućinama i odjeljcima. Izračunato je da je ceftriakson u febrilnih ovaca najbolje davati intravenski u dozi od 15 mg/kg tjelesne mase u razmaku od pet sati

Variance components of combining ability for different morpho-physiological traits for heat tolerance in bread wheat

To estimate the level of heat tolerance for different genotypes of bread wheat with respect to morphological characters under studied grains/ spike, grain weight/spike, grain filling duration (duration between the anthesis stage and the physiological maturity), 1000-kernel weight and grain yield/plant for yield. Physiological traits like relative injury (RI %), chlorophyll content, canopy temperature depression (CTD), were used in present investigation to contribute toward capability of plants to tolerate heat stress of the yield contributing traits during heat stress.The findings of present investigation had clearly explained that influences of environments on morpho physiological characters i.e. grain yield per plant (14886.15) and its attributing traits i.e. spike length (459.7), tillers per plant (622.34), spikelets per spike (278.1), 1000 kernel weight (13262.39), grain weight per spike (177.89) and number of grains per spike (2898.44) in wheat were highly significant and positive. Among the parent and their crosses had handsome amount of variations across the environment. The results of interaction for environments with parents, lines, testers and their crosses with respect to morpho physiological characters in wheat was found significant for some characters while variation was absent for other characters studied. Physiological traits like relative injury per cent, chlorophyll content and CTD were vital parameters to quantify the degree of heat stress to develop tolerant genotypes which is urgent and present need under changing climate scenario

BHUx: A Patent Polyherbal Formulation to Prevent Atherosclerosis

Ayurvedic medicine is a time-tested system of medicine which has been in clinical use for centuries in India. Being a time-tested system, it has an edge over other existing systems of health management, especially for dealing with chronic disorders such as coronary artery disease, which is of a complex multi-etiological nature. Recently, we have shown that BHUx, a patented polyherbal formulation consisting of the aqueous fraction of five medicinal plants of the ayurvedic system, has significant anti-inflammatory properties through inhibition of cyclooxygenase-2 and lipoxygenase-15. Here we have investigated its effect on diet-induced atherosclerosis in albino rabbits. BHUx was given orally for 3 months to rabbits pre-treated with an atherogenic diet for 3 months. After 6 months, the dorsal aorta was processed for histological studies for calcium and collagen content. The results demonstrated a remarkable reduction in intimal thickening in the treated animals. In addition, there was less calcification at the intima–medial interface and increased intensity of collagen cap on the surface along with an increase in survival, compared with the sham control. We suggest that BHUx is a potent, multi-factorial formulation against atherosclerosis

MOLECULAR DOCKING STUDIES OF SOME NOVEL THIOPHENE CARBOHYDRAZIDE DERIVATIVES ON ENTEROTOXIN OF BACILLUS CEREUS

Objective: In this work, we collected the three-dimensional structure of Enterotoxin from Bacillus cereus which plays an important role in the pathway. Methods: The protein structure was collected from PDB data bank. From the 3D structures of the proteins, the targeted derivatives were designed. Docking studies were performed with designed compounds. Results: The compounds docked to the protein by hydrogen bonding interactions and these interactions play an important role in the binding studies. Docking results showed the best compounds among the derivatives. Conclusion: The docking results agreed well with the observed in vitro data, in which the anti-microbial activity of the analogs was higher than other drugs and formed hydrogen bonds

Synthesis and Antimicrobial Activity of Some Novel Thiophene Carbohydrazide Derivatives

ABSTRACT The present paper describes the synthesis and antibacterial activity of fifteen novel (E)-N'-benzylidene-3-methoxy-4(4-nitrophenyl)thiophene) benzohydrazide derivatives (5a-m) from commercially available methyl-4-bromo-3-methoxythiophene-2-carboxylate as starting material. Th

Warm-water Dasycladaceae algae from the Late Ordovician of the Parahio Valley, Spiti, India

Warm-water Dasycladaceae algae Mastopora and Cyclocrinites were for the first time recorded from the Takche Formation (Upper Ordovician–lower Silurian), Parahio Valley, Spiti, India. They are preserved as external and internal moulds of the non-globular or possibly bulb-like cortical skeleton showing flattened thalli with a high degree of compaction. The occurrence of abundant cyclocrinitid remains in the Takche Formation indicates that the Spiti region of the northwestern Himalaya must have been located at about 30° palaeolatitude during the Late Ordovician and early Silurian. The cyclocrinitids were warm-water algae and their extinction at the end of the Ordovician is related to cooling and glaciations. The cyclocrinitids in the Ordovician are known from several localities in central and southern Asia, including Kazakhstan and western China

Serosurveillance for Japanese encephalitis virus infection among equines in India

The seroprevalence of Japanese encephalitis virus (JEV) among equines was evaluated from January 2006 to December 2009 in 13 different states of India by hemagglutination inhibition (HI) test and virus neutralization test (VNT). Antibodies against JEV were detected in 327 out of 3,286 (10%) equines with a maximum prevalence reported in the state of Manipur (91.7%) followed by Gujarat (18.5%), Madhya Pradesh (14.4%), and Uttar Pradesh (11.6%). Evidence of JEV infection was observed in equines in Indore (Madhya Pradesh) where a 4-fold or higher rise in antibody titer was observed in 21 out of 34 horses in November 2007 to October 2006. In March 2008, seven of these horses had a subsequent 4-fold rise in JEV antibody titers while this titer decreased in nine animals. JEV-positive horse sera had a JEV/WNV (West Nile virus) ratio over 2.0 according to the HI and/or VNT. These results indicated that JEV is endemic among equines in India

Ethnomedicinal values of Boerhaavia diffusa L. as a panacea against multiple human ailments: a state of art review

Ethnopharmacological relevance: Therapeutic botanicals (plants and derivatives) are in use since antiquity for various health ailments. The ethnic community is the repository of the information, the multifactorial therapeutic applications of which may often need scientific validation. The spreading hogweed or Boerhaavia diffusa L., also known as Punarnava, is a reassuring medicinal herb with diverse pharmacological benefits. It is used in Ayurveda in Asia and Africa as a rejuvenator or “Rasayan” for its excellent antiaging and antioxidant properties.Aim: The study aimed at compiling the state-of-art knowledge of the medicinal benefits of Boerhaavia diffusa L. and unraveling the unexplored commercially useful bioactive constituents by establishing their possible pharmacological benefits.Methods: The data from published literature, confined to pharmacological manifestations of various phytocomponents of Boerhaavia diffusa L. or its parts like root, leaf and stem were extracted from scientific databases, Google, Science Direct, PubMed, etc. using its antifungal, antibacterial, anticancer, anti-inflammatory, antidiabetic, hepatoprotective, cardioprotective, renoprotective, antifertility benefits and molecular docking study as search strings and keywords. Further, the reported in silico studies for bioactivity and bioavailability are detailed.Results: The botanicals possess numerous bioactive compounds, the most widely reported ones being phenolic (punarnavoside, trans-caftaric acid, boerhavic acid), rotenoid (boeravinones A-J), flavonoid (borhaavone, quercetin, kaempferol), isoflavonoid (2′-O-methyl abronisoflavone), alkaloid (punarnavine), steroid (boerhavisterol, β-Ecdysone), anthracenes and lignans (liriodendrin, syringaresinol mono-β-D-glucoside). Some of the reported reassuring benefits of their purified forms or even the crude extracts are antidiabetic, antimicrobial, anticancer, antioxidant, anti-inflammatory, hepatoprotective, renoprotective, cardioprotective, antifertility, etc.Conclusion: The article provides an extensive study on such pharmacological utility to support the ethnomedicinal use of Boerhaavia diffusa L. and propose possible mechanism of the various bioactive compounds in optimising metabolic dysfunctions, healing and protecting vital body organs, often related to the magnificent antioxidant property of this ayurvedic panacea. Further, establishing specific roles of its yet-to-explore bioactive constituents for diverse pharmacological applications is suggested