Epidemiology of Breed-Related Mast Cell Tumour Occurrence and Prognostic Significance of Clinical Features in a Defined Population of Dogs in West-Central Italy

Abstract: Canine mast cell tumours (MCTs) present a wide variety of challenging clinical behaviours in terms of predicting the prognosis and choosing appropriate treatment. This study investigated the frequency, risk, and prognostic factors of MCTs in dogs admitted to a single veterinary teaching hospital (VTH). Breed, age, sex, and sexual status in ninety-eight dogs with MCTs (MCT-group) were compared with a control group of 13,077 dogs (VTH-group) obtained from the VTH clinical database from January 2010 to January 2016. Within the MCT-group, signalment, location, size, mass number, ulceration, histopathological grading, presence of lymph node, or distant metastases were compared with each other and with the outcome. Boxers (OR 7.2), American Pit Bull Terriers (OR 5.4), French Bulldogs (OR 4.4) and Labrador Retrievers (OR 2.6) were overrepresented. The MCT-group was significantly older than the VTH-group (p < 0.0001). In comparison with the VTH group, in the MCT-group neutered dogs (OR 2.1) and spayed females (OR 2.3) were predominant compared to intact dogs and intact females, respectively. Ulceration (OR 5.2) and lymph node metastasis (OR 7.1) occurred more frequently in larger MCTs. Both ulceration and MCTs > 3 cm were highly associated with lymph node metastasis (OR 24.8). Recurrence was associated with MCT-related death (OR 10.50, p = 0.0040), and the latter was associated with shorter survival times (p = 0.0115). Dogs with MCTs > 3 cm (p = 0.0040), lymph node metastasis (p = 0.0234), or elevated WHO stage (p = 0.0158) had shorter survival times. A significantly higher frequency of MCTs was found in specific breeds, and in older and neutered dogs. MCTs > 3 cm and lymph node or distant metastases were associated with shorter survival times

Primary leiomyosarcoma of the jugular vein in a dog

A four-year-old, male, Labrador retriever was referred for removal of a spindle cell sarcoma involving the right jugular vein. A post-contrast CT scan showed a seven-centimeter subcutaneous mass originated from the right external jugular vein, which was partially obstructed and showing contrast stasis, suggested a primary intravascular tumor of the jugular vein. The mass was resected, and histological evaluation was consistent with grade II intravenous spindle cell sarcoma of the jugular vein. Immunohistochemical positivity for vimentin, desmin, and αSMA antibody and negativity for S-100 protein confirmed venous leiomyosarcoma. The dog received five doses of intravenous doxorubicin, and there was no recurrence of the tumor 30 months post treatment. In dogs, primary intravascular sarcomas are rare and primary venous leiomyosarcoma has not been described. A venous tumor may be considered as a differential diagnosis in dogs with ventral neck swelling

Two cases of feline paraneoplastic alopecia associated with a neuroendocrine pancreatic neoplasia and a hepatosplenic plasma cell tumour

Background: Feline paraneoplastic alopecia (FPA) is a rare condition listed among the cutaneous paraneoplastic syndromes, which occurs in association with pancreatic carcinoma, cholangiocarcinoma, hepatocellular carcinoma and metastatic intestinal carcinoma. Objectives: To describe the clinicopathological findings of paraneoplastic alopecia in two cats each with an uncommon tumour not previously reported in association with FPA. Animals: Paraneoplastic alopecia was associated with neuroendocrine pancreatic neoplasia in a Persian cat and with a hepatosplenic plasma cell tumour in a domestic short hair cat. Results: FPA was suspected based on age, rapid onset of clinical signs, ventral distribution of alopecia, shiny appearance of the skin and telogenization/miniaturization of the follicles on histopathology. The nature of the tumours was determined through cytology, postmortem, histopathological and immunohistochemical examination, and capillary immunoelectrophoresis. A causative association between the skin lesions and the tumour was suggested by clinical and histopathological features shared with previously published cases. Conclusions and clinical importance: Pancreatic neuroendocrine and plasma cell tumour should be considered as differential diagnoses when evaluating FPA

Laryngeal paralysis associated with a muscle pseudotumour in a young dog

An 18-month-old male entire Bloodhound dog was presented with a six-week history of progressive inspiratory dyspnoea, stridor, dysphonia and exercise intolerance. CT scan performed elsewhere had revealed the presence of an unencapsulated nodular mass (3x1x5 cm) dorsal to the larynx and first tracheal rings. Laryngoscopy demonstrated the presence of bilateral laryngeal paralysis and distorted laryngeal architecture suggestive of extraluminal compression. Histopathology results of incisional biopsies from the mass were suggestive of a benign non-neoplastic muscular lesion. Surgery was performed to manage laryngeal paralysis and attempt mass excision. A second histopathology examination confirmed an inflammatory and dysplastic lesion suggestive of a pseudotumour. All clinical signs resolved after surgery and at the 13 months follow-up the dog remains asymptomatic. To the authors’ knowledge, this is the first report of a case of laryngeal paralysis caused by a muscle pseudotumour in a young dog.Keywords: Dog, Laryngeal paralysis, Larynx, Muscle pseudotumou

Feline leishmaniosis: A case with a high parasitic burden

Leishmaniosis is a widespread zoonotic disease with the potential for significant impact on public health. Although dogs are the main reservoir host, the role of other domestic species in transmission of the disease should be considered, and felids may represent an alternative source of infection.1 We describe a case of Leishmania infantum infection in a cat with oral lesions associated with a high parasitic load detected by a quantitative PCR technique. A 5-year-old male short-haired cat was presented for evaluation of respiratory signs, conjunctivitis and oral lesions. Clinical examination revealed a poor-quality hair coat, bilateral conjunctivitis, nasal exudate and a diffuse hyperplastic and ulcerative stomatitis. Complete blood count and serum protein electrophoresis values were within normal range, and screening tests for feline leukaemia virus and feline immunodeficiency virus were negative. Histopathological evaluation of biopsy tissue from the oral lesion confirmed severe, diffuse granulomatous stomatitis, with numerous macrophages containing intracytoplasmic protozoal organisms (Figure 1). Serology was negative for Toxoplasma and Neospora and positive for Leishmania by an indirect fluorescent antibody titre of 1:320.2 A diagnosis of feline leishmaniosis was confirmed

Feline leishmaniosis: a case with a high parasitic burden

Leishmaniosis is a widespread zoonotic disease with the potential for significant impact on public health. Although dogs are the main reservoir host, the role of other domestic species in transmission of the disease should be considered, and felids may represent an alternative source of infection.1 We describe a case of Leishmania infantum infection in a cat with oral lesions associated with a high parasitic load detected by a quantitative PCR technique. A 5-year-old male short-haired cat was presented for evaluation of respiratory signs, conjunctivitis and oral lesions. Clinical examination revealed a poor-quality hair coat, bilateral conjunctivitis, nasal exudate and a diffuse hyperplastic and ulcerative stomatitis. Complete blood count and serum protein electrophoresis values were within normal range, and screening tests for feline leukaemia virus and feline immunodeficiency virus were negative. Histopathological evaluation of biopsy tissue from the oral lesion confirmed severe, diffuse granulomatous stomatitis, with numerous macrophages containing intracytoplasmic protozoal organisms (Figure 1). Serology was negative for Toxoplasma and Neospora and positive for Leishmania by an indirect fluorescent antibody titre of 1:320.2 A diagnosis of feline leishmaniosis was confirmed

A study of multiple Felis catus papillomavirus types (1, 2, 3, 4) in cat skin lesions in Italy by quantitative PCR

Objectives The aim of the study was to investigate, by quantitative PCR (qPCR) the presence of papillomavirus in feline viral plaques (VPs), Bowenoid in situ carcinoma (BISC), squamous cell carcinoma (SCC) and actinic keratosis (AK). Methods Twenty-nine cases with previously established diagnoses of feline VPs, BISC, invasive SCC and AK were selected from a dermatopathological database. A critical re-evaluation of diagnosis was performed by defining clear criteria toward carcinomatous vs non-carcinomatous, in situ vs invasive (if carcinomatous) and viral vs actinic. Cases were evaluated for p16 immunolocalisation. The presence of the target viral genes for Felis catus papillomavirus (FcaPV)-1, FcaPV-2, FcaPV-3 and FcaPV-4 was determined by qPCR. The data generated ΔΔCq values, which represent a normalised measure of DNA viral quantity. Samples with a positive ΔΔCq value were submitted to sequence analysis. Results Four VPs, 19 BISCs, four SCCs and one AK were included. By ΔΔCq analysis we found that all VPs were positive for FcaPV-1 or FcaPV-2; eight BISCs were positive for FcaPV-1, FcaPV-2 and FcaPV-4. FcaPV-2 was the most prevalent among the group of VPs and BISCs. Conclusions and relevance Using the ΔΔCq method we report the first evidence of FcaPV-1, FcaPV-2 and FcaPV-4 in Italy; FcaPV-2 was the most frequently detected. To a lesser extent, FcaPV-1 and FcaPV-4 were detected in the examined samples. FcaPV-3 was never associated with viral-induced lesions by ΔΔCq investigation. Compared with conventional PCR the ΔΔCq method has the advantage of establishing a possible role of the virus in the outcome of infection

Canine Clitoral Carcinoma: A Clinical, Cytologic, Histopathologic, Immunohistochemical, and Ultrastructural Study

Vaginal and vulvar tumors are uncommon in dogs. Knowledge of canine primary clitoral neoplasia is restricted to a few case reports, and only carcinomas have been reported. Cytologic and histologic features reported in the literature seem to overlap with those of canine apocrine gland anal sac adenocarcinoma (AGASA). Clinical features also recall those of canine AGASA, such as locoregional metastases and hypercalcemia of malignancy (HM). In this study, 6 cases of primary canine clitoral carcinomas (CCCs), with and without HM, were investigated by means of cytology, histopathology, electron microscopy, and immunohistochemistry for neuroendocrine markers including chromogranin A (CGA), synaptophysin (SYN), neuron-specific enolase (NSE), and S-100. In all 6 tumors, cytologic findings were consistent with malignant epithelial neoplasia of apocrine gland origin. The tumors examined were classified into 3 different histological patterns representing different degrees of differentiation: tubular, solid, and rosette type. Both CGA and SYN were mildly expressed in 2 of 6 tumors, while NSE was consistently expressed in all 6 cases. None of the tumors were S-100 positive. Transmission electron microscopy revealed electron-dense cytoplasmic granules compatible with neuroendocrine granules in all 6 cases. CCCs presented clinicopathologic features resembling AGASAs with neuroendocrine characteristics, and 2 of 6 neoplasms were considered as carcinomas with neuroendocrine differentiation and were positive for 3 neuroendocrine markers. CCCs can often present with HM, and long-term outcome is likely poor. Our study concludes that CCC seems to be a rare tumor, but it might be underestimated because of the overlapping features with AGASA. Further studies should aim to define the true incidence of this disease