Band-collision gel electrophoresis.

Electrophoretic mobility shift assays are widely used in gel electrophoresis to study binding interactions between different molecular species loaded into the same well. However, shift assays can access only a subset of reaction possibilities that could be otherwise seen if separate bands of reagent species might instead be collisionally reacted. Here, we adapt gel electrophoresis by fabricating two or more wells in the same lane, loading these wells with different reagent species, and applying an electric field, thereby producing collisional reactions between propagating pulse-like bands of these species, which we image optically. For certain pairs of anionic and cationic dyes, propagating bands pass through each other unperturbed; yet, for other pairs, we observe complexing and precipitation reactions, indicating strong attractive interactions. We generalize this band-collision gel electrophoresis (BCGE) approach to other reaction types, including acid-base, ligand exchange, and redox, as well as to colloidal species in passivated large-pore gels

Current Trends in Student Assessment Practices and Their Underlying Philosophy

fter we explain, initially, the differences between assessing the learning process and assessing a student’s performance, we analyse all the parameters on the basis of which a student and his/her performance are assessed. Finally, we present and evaluate all current trends that modernise the assessment methods and techniques related to the learning process and student performance, and also stress the basic logic and philosophy that govern these new approaches and also the points that make them different from what was applied in the field of student assessment until recently

ReCentering Psych Stats

To center a variable in regression means to set its value at zero and interpret all other values in relation to this reference point. Regarding race and gender, researchers often center male and White at zero. Further, it is typical that research vignettes in statistics textbooks are similarly seated in a White, Western (frequently U.S.), heteronormative, framework. ReCentering Psych Stats seeks provide statistics training for psychology students (undergraduate, graduate, and post-doctoral) in a socially and culturally responsive way. All lessons use the open-source statistics program, R (and its associated packages). Each lesson includes a chapter and screencasted lesson, features a workflow for statistical decision-making, and includes all R code necessary to conduct the statistic. Research vignettes are drawn from the published psychology literature. When possible, these articles are authored by individuals who hold identities that have, been marginalized in the scientific literature; correctly use the statistic that is being taught in the lesson; and focus on issues of justice, equity, inclusion, or and diversity. When possible, lessons include interviews with researchers from the featured vignettes. Each chapter includes suggestions for practice that are graded in complexity, such that learners can choose the degree of challenge. ReCentering Psych Stats is perpetually-in-progress; suggestions for corrections or chapters are welcomed: [email protected] Link to full online book and supplemental materials (first page includes links to .pdf, .doc, and ebook formats): https://lhbikos.github.io/ReCenterPsychStats/https://digitalcommons.spu.edu/open_books/1003/thumbnail.jp

I Took the Blue Pill The Effect of the Hegemonic Masculine Police Culture on Canadian Policewomen\u27s Identities

There are varied opinions as to whether the subculture of policing continues to reproduce traditional gender roles and stereotypes in order to maintain male dominance, leaving policewomen at a distinct disadvantage. In an effort to understand this phenomenon from policewomen’s own experiences, this study utilized qualitative in-depth interviews with 15 policewomen from varied police forces in Southwestern Ontario, Canada. The role of police culture and hegemonic masculinity is explored in relation to the identity formation of policewomen both on and off-duty. Drawing on the work of Dorothy Smith (1987), the findings reveal that policewomen have a bifurcated consciousness, dividing the world as they actually experience it from the hegemonic masculine view they adopt as officers. Since the latter viewpoint strongly devalues the former, women are frequently conflicted, and at times at war with themselves and each other. The results confirm that the hegemonic masculine values perpetuated by the institution of policing influences the way policewomen see themselves, the world, and each other

“It\u27s all window dressing:” Canadian police officers\u27 perceptions of mental health stigma in their workplace

Purpose: This study will provide a preliminary, general overview of Canadian police officers’ perception of stigma toward mental illness in their workplace culture and its impacts. Design/methodology/approach: This study uses a mixed methods approach with two nationwide datasets: a self-report survey (N=727) and 116 semi-structured interviews with police officers from 31 police services. Results are grounded in theories of stigma, masculinities, and organizational culture. Findings: Results indicate that most officers believe stigma toward mental illness in their workplace remains, despite senior management messaging and program implementation. Reporting mental illness was often seen as high risk, both personally and professionally. Policewomen, constables, and those on leave reported statistically significant higher levels of perceived stigma and risk. Features of traditional masculinity were commonly reported, influencing the way individuals viewed themselves (self-stigma) and organizational response (structural stigma). Those with lived experience reported the highest levels of self and structural stigmatization, which often negatively impacted their recovery. Originality/value This study strengthens our understanding of how organizational culture and structure combine to contribute to the persistent presence of stigma in some Canadian police services (with implications for male-dominated occupations generally). Gender, rank, years of service and lived experience are additional areas of limited scholarship addressed by this study. The findings have important implications for effective program and policy evaluation and development

MaskMaker, MaskMaker, Make Me a Mask: A study of the effect of resilient coping behavior on positive and negative affect during COVID-19.

During times of crisis, resilient coping behavior may be an important pathway for improving positive and negative affect. This relationship is explored via a moderated mediation model using data from a survey administered to volunteer maskmaskers during COVID-19. We hypothesize there will be a significant effect of resilient coping on affect, mediated through mask making hours. Change to employment is likely to moderate this indirect effect as well as interact directly with the coping to affect relationship. If supported, our hypotheses support the notion that engaging in a prosocial behavior such as Maskmaking may contribute positively to mental health outcomes

Determining the temporal direction of psychological distress and substance use in female expatriate spouses in Turkey

Expatriation has been associated with a number of negative mental health issues within the expats themselves (e.g., depression, substance use). However, expatriate spouses can often face unique stressors, and by gaining a better understanding of the impact expatriation has on these spouses, organizations can more easily plan for the challenges. The purpose of our study is to further explore the direction of the relationship between alcohol use and psychological distress in a sample of female expatriate spouses during their first year assignment in Turkey. By using hierarchical linear modeling and conducting a cross-lead analysis, we will analyze the change over time for alcohol use and psychological distress

The Psychology of Hope

2007/10/24. Discusses psychological concepts of hope and outlines building and maintaining hope in one\u27s own life. Associate Professor of Clinical Psychology and Director of Research. Doctoral student. Sixth Annual Day of Common Learning

Cultural differences in coping self-efficacy, perceived social support, and satisfaction with their legal advocate in individuals who have experienced sexual assault

The King County Sexual Assault Resource Center’s (KCSARC) legal advocacy program assists individuals who have been sexually assaulted to navigate the justice system and serves a diverse clientele. Research suggests that there exists differences between ethnic groups in the qualitative psychological reactions to trauma, prevalence of post-trauma victimization, and use of therapeutic and tangible services. Thus, the purpose of our study is to examine the association between ethnicity, the three critical outcome variables (coping self-efficacy, perceived social support, and legal advocate satisfaction) and posttraumatic recovery