Pathological regional blood flow in opiate-dependent patients during withdrawal: A HMPAO-SPECT study

The aims of the present study were to investigate regional cerebral blood flow (rCBF) in heroin-dependent patients during withdrawal and to assess the relation between these changes and duration of heroin consumption and withdrawal data. The rCBF was measured using brain SPECT with Tc-99m-HMPAO in 16 heroin-dependent patients during heroin withdrawal. Thirteen patients received levomethadone at the time of the SPECT scans. The images were analyzed both visually and quantitatively, a total of 21 hypoperfused brain regions were observed in 11 of the 16 patients. The temporal lobes were the most affected area, hypoperfusions of the right and left temporal lobe were observed in 5 and 5 patients, respectively. Three of the patients had a hypoperfusion of the right frontal lobe, 2 patients showed perfusion defects in the left frontal lobe, right parietal lobe and left parietal lobe. The results of the quantitative assessments of the rCBF were consistent with the results of the qualitative findings. The stepwise regression analysis showed a significant positive correlation (r = 0.54) between the dose of levomethadone at the time of the SPECT scan and the rCBF of the right parietal lobe. Other significant correlations between clinical data and rCBF were not found. The present results suggest brain perfusion abnormalities during heroin withdrawal in heroin-dependent patients, which are not due to the conditions of withdrawal

Entropically-driven binding of mithramycin in the minor groove of C/G-rich DNA sequences

Final full-text version available at: http://dx.doi.org/10.1093/nar/gkm037.-- Supplementary Data is available.The antitumour antibiotic mithramycin A (MTA) is a DNA minor-groove binding ligand. It binds to C/G-rich tracts as a dimer that forms in the presence of divalent cations such as Mg2+. Differential scanning calorimetry, UV thermal denaturation, isothermal titration calorimetry and competition dialysis were used, together with computations of the hydrophobic free energy of binding, to determine the thermodynamic profile of MTA binding to DNA. The results were compared to those obtained in parallel using the structurally related mithramycin SK (MSK). The binding of MTA to salmon testes DNA determined by UV melting studies (Kobs = 1.2 (±0.3) x 10^5 M–1) is tighter than that of MSK (2.9 (±1.0) x 10^4 M–1) at 25°C. Competition dialysis studies showed a tighter MTA binding to both salmon testes DNA (42% C + G) and Micrococcus lysodeikticus DNA (72% C + G). The thermodynamic analysis of binding data at 25°C shows that the binding of MTA and MSK to DNA is entropically driven, dominated by the hydrophobic transfer of the antibiotics from solution to the DNA-binding site. Direct molecular recognition between MTA or MSK and DNA through hydrogen bonding and van der Waals contacts may also contribute significantly to complex formation.Supported by a grant from the Spanish Ministry of Education and Science (SAF2005-00551) and the FEDER program of the European Community. This work was carried out within the framework of the Centre de Referencia en Biotecnologia of the Generalitat de Catalunya. Funding to pay the Open Access publication charge was provided by the Ministry of Education and Science and CSIC (Spain).Peer reviewe

Repeated Radionuclide therapy in metastatic paraganglioma leading to the highest reported cumulative activity of 131I-MIBG

131I-MIBG therapy for neuroendocrine tumours may be dose limited. The common range of applied cumulative activities is 10-40 GBq. We report the uneventful cumulative administration of 111 GBq (= 3 Ci) 131I-MIBG in a patient with metastatic paraganglioma. Ten courses of 131I-MIBG therapy were given within six years, accomplishing symptomatic, hormonal and tumour responses with no serious adverse effects. Chemotherapy with cisplatin/vinblastine/dacarbazine was the final treatment modality with temporary control of disease, but eventually the patient died of progression. The observed cumulative activity of 131I-MIBG represents the highest value reported to our knowledge, and even though 12.6 GBq of 90Y-DOTATOC were added intermediately, no associated relevant bone marrow, hepatic or other toxicity were observed. In an individual attempt to palliate metastatic disease high cumulative activity alone should not preclude the patient from repeat treatment

Inter-Destination Multimedia Synchronization; Schemes, Use Cases and Standardization

Traditionally, the media consumption model has been a passive and isolated activity. However, the advent of media streaming technologies, interactive social applications, and synchronous communications, as well as the convergence between these three developments, point to an evolution towards dynamic shared media experiences. In this new model, geographically distributed groups of consumers, independently of their location and the nature of their end-devices, can be immersed in a common virtual networked environment in which they can share multimedia services, interact and collaborate in real-time within the context of simultaneous media content consumption. In most of these multimedia services and applications, apart from the well-known intra and inter-stream synchronization techniques that are important inside the consumers playout devices, also the synchronization of the playout processes between several distributed receivers, known as multipoint, group or Inter-destination multimedia synchronization (IDMS), becomes essential. Due to the increasing popularity of social networking, this type of multimedia synchronization has gained in popularity in recent years. Although Social TV is perhaps the most prominent use case in which IDMS is useful, in this paper we present up to 19 use cases for IDMS, each one having its own synchronization requirements. Different approaches used in the (recent) past by researchers to achieve IDMS are described and compared. As further proof of the significance of IDMS nowadays, relevant organizations (such as ETSI TISPAN and IETF AVTCORE Group) efforts on IDMS standardization (in which authors have been and are participating actively), defining architectures and protocols, are summarized.This work has been financed, partially, by Universitat Politecnica de Valencia (UPV), under its R&D Support Program in PAID-05-11-002-331 Project and in PAID-01-10, and by TNO, under its Future Internet Use Research & Innovation Program. The authors also want to thank Kevin Gross for providing some of the use cases included in Sect. 1.2.Montagud, M.; Boronat Segui, F.; Stokking, H.; Van Brandenburg, R. (2012). Inter-Destination Multimedia Synchronization; Schemes, Use Cases and Standardization. Multimedia Systems. 18(6):459-482. https://doi.org/10.1007/s00530-012-0278-9S459482186Kernchen, R., Meissner, S., Moessner, K., Cesar, P., Vaishnavi, I., Boussard, M., Hesselman, C.: Intelligent multimedia presentation in ubiquitous multidevice scenarios. IEEE Multimedia 17(2), 52–63 (2010)Vaishnavi, I., Cesar, P., Bulterman, D., Friedrich, O., Gunkel, S., Geerts, D.: From IPTV to synchronous shared experiences challenges in design: distributed media synchronization. Signal Process Image Commun 26(7), 370–377 (2011)Geerts, D., Vaishnavi, I., Mekuria, R., Van Deventer, O., Cesar, P.: Are we in sync?: synchronization requirements for watching on-line video together, CHI ‘11, New York, USA (2011)Boronat, F., Lloret, J., García, M.: Multimedia group and inter-stream synchronization techniques: a comparative study. Inf. Syst. 34(1), 108–131 (2009)Chen, M.: A low-latency lip-synchronized videoconferencing system. In: SIGCHI Conference on Human Factors in Computing Systems, CHI’03, ACM, pp. 464–471, New York (2003)Ishibashi, Y., Tasaka, S., Ogawa, H.: Media synchronization quality of reactive control schemes. IEICE Trans. Commun. E86-B(10), 3103–3113 (2003)Ademoye, O.A., Ghinea, G.: Synchronization of olfaction-enhanced multimedia. IEEE Trans. Multimedia 11(3), 561–565 (2009)Cesar, P., Bulterman, D.C.A., Jansen, J., Geerts, D., Knoche, H., Seager, W.: Fragment, tag, enrich, and send: enhancing social sharing of video. ACM Trans. Multimedia Comput. Commun. Appl. 5(3), Article 19, 27 pages (2009)Van Deventer, M.O., Stokking, H., Niamut, O.A., Walraven, F.A., Klos, V.B.: Advanced Interactive Television Service Require Synchronization, IWSSIP 2008. Bratislava, June (2008)Premchaiswadi, W., Tungkasthan, A., Jongsawat, N.: Enhancing learning systems by using virtual interactive classrooms and web-based collaborative work. In: Proceedings of the IEEE Education Engineering Conference (EDUCON 2010), pp. 1531–1537. Madrid, Spain (2010)Diot, C., Gautier, L.: A distributed architecture for multiplayer interactive applications on the internet. IEEE Netw 13(4), 6–15 (1999)Mauve, M., Vogel, J., Hilt, V., Effelsberg, W.: Local-lag and timewarp: providing consistency for replicated continuous applications. IEEE Trans. Multimedia 6(1), 45–57 (2004)Hosoya, K., Ishibashi, Y., Sugawara, S., Psannis, K.E.: Group synchronization control considering difference of conversation roles. In: IEEE 13th International Symposium on Consumer Electronics, ISCE ‘09, pp. 948–952 (2009)Roccetti, M., Ferretti, S., Palazzi, C.: The brave new world of multiplayer online games: synchronization issues with smart solution. In: 11th IEEE Symposium on Object Oriented Real-Time Distributed Computing (ISORC), pp. 587–592 (2008)Ott, D.E., Mayer-Patel, K.: An open architecture for transport-level protocol coordination in distributed multimedia applications. ACM Trans. Multimedia Comput. Commun. Appl. 3(3), 17 (2007)Boronat, F., Montagud, M., Guerri, J.C.: Multimedia group synchronization approach for one-way cluster-to-cluster applications. In: IEEE 34th Conference on Local Computer Networks, LCN 2009, pp. 177–184, Zürich (2009)Boronat, F., Montagud, M., Vidal, V.: Smooth control of adaptive media playout to acquire IDMS in cluster-based applications. In: IEEE LCN 2011, pp. 617–625, Bonn (2011)Huang, Z., Wu, W., Nahrstedt, K., Rivas, R., Arefin, A.: SyncCast: synchronized dissemination in multi-site interactive 3D tele-immersion. In: Proceedings of MMSys, USA (2011)Kim, S.-J., Kuester, F., Kim, K.: A global timestamp-based approach for enhanced data consistency and fairness in collaborative virtual environments. ACM/Springer Multimedia Syst. J. 10(3), 220–229 (2005)Schooler, E.: Distributed music: a foray into networked performance. In: International Network Music Festival, Santa Monica, CA (1993)Miyashita, Y., Ishibashi, Y., Fukushima, N., Sugawara, S., Psannis K.E.: QoE assessment of group synchronization in networked chorus with voice and video. In: Proceedings of IEEE TENCON’11, pp. 393–397 (2011)Hesselman, C., Abbadessa, D., Van Der Beek, W., et al.: Sharing enriched multimedia experiences across heterogeneous network infrastructures. IEEE Commun. Mag. 48(6), 54–65 (2010)Montpetit, M., Klym, N., Mirlacher, T.: The future of IPTV—Connected, mobile, personal and social. Multimedia Tools Appl J 53(3), 519–532 (2011)Cesar, P., Bulterman, D.C.A., Jansen, J.: Leveraging the user impact: an architecture for secondary screens usage in an interactive television environment. ACM/Springer Multimedia Syst. 15(3), 127–142 (2009)Lukosch, S.: Transparent latecomer support for synchronous groupware. In: Proceedings of 9th International Workshop on Groupware (CRIWG), Grenoble, France, pp. 26–41 (2003)Steinmetz, R.: Human perception of jitter and media synchronization. IEEE J. Sel. Areas Commun. 14(1), 61–72 (1996)Stokking, H., Van Deventer, M.O., Niamut, O.A., Walraven, F.A., Mekuria, R.N.: IPTV inter-destination synchronization: a network-based approach, ICIN’2010, Berlin (2010)Mekuria, R.N.: Inter-destination media synchronization for TV broadcasts, Master Thesis, Faculty of Electrical Engineering, Mathematics and Computer Science, Department of Network architecture and Services, Delft University of Technology (2011)Pitt Ian, CS2511: Usability engineering lecture notes, localisation of sound sources. http://web.archive.org/web/20100410235208/http:/www.cs.ucc.ie/~ianp/CS2511/HAP.htmlNielsen, J.: Response times: the three important limits. http://www.useit.com/papers/responsetime.html (1994)ITU-T Rec G. 1010: End-User Multimedia QoS Categories. International Telecommunication Union, Geneva (2001)Biersack, E., Geyer, W.: Synchronized delivery and playout of distributed stored multimedia streams. ACM/Springer Multimedia Syst 7(1), 70–90 (1999)Xie, Y., Liu, C., Lee, M.J., Saadawi, T.N.: Adaptive multimedia synchronization in a teleconference system. ACM/Springer Multimedia Syst. 7(4), 326–337 (1999)Laoutaris, N., Stavrakakis, I.: Intrastream synchronization for continuous media streams: a survey of playout schedulers. IEEE Netw. Mag. 16(3), 30–40 (2002)Ishibashi, Y., Tsuji, A., Tasaka, S.: A group synchronization mechanism for stored media in multicast communications. In: Proceedings of the INFOCOM ‘97, Washington (1997)Ishibashi, Y., Tasaka, S.: A group synchronization mechanism for live media in multicast communications. IEEE GLOBECOM’97, pp. 746–752 (1997)Boronat, F., Guerri, J.C., Lloret, J.: An RTP/RTCP based approach for multimedia group and inter-stream synchronization. Multimedia Tools Appl. J. 40(2), 285–319 (2008)Ishibashi, I., Tasaka, S.: A distributed control scheme for group synchronization in multicast communications. In: Proceedings of International Symposium Communications, Kaohsiung, Taiwan, pp. 317–323 (1999)Lu, Y., Fallica, B., Kuipers, F.A., Kooij, R.E., Van Mieghem, P.: Assessing the quality of experience of SopCast. Int. J. Internet Protoc. Technol 4(1), 11–19 (2009)Shamma, D.A., Bastea-Forte, M., Joubert, N., Liu, Y.: Enhancing online personal connections through synchronized sharing of online video, ACM CHI’08 Extended Abstracts, Florence (2008)Ishibashi, Y., Tasaka, S.: A distributed control scheme for causality and media synchronization in networked multimedia games. In: Proceedings of 11th International Conference on Computer Communications and Networks, pp. 144–149, Miami, USA (2002)Ishibashi, Y., Tomaru, K., Tasaka, S., Inazumi, K.: Group synchronization in networked virtual environments. In: Proceedings of the 38th IEEE International Conference on Communications, pp. 885–890, Alaska, USA (2003)Tasaka, S., Ishibashi, Y., Hayashi, M.: Inter–destination synchronization quality in an integrated wired and wireless network with handover. IEEE GLOBECOM 2, 1560–1565 (2002)Kurokawa, Y., Ishibashi, Y., Asano, T.: Group synchronization control in a remote haptic drawing system. In: Proceedings of IEEE International Conference on Multimedia and Expo, pp. 572–575, Beijing, China (2007)Hashimoto, T., Ishibashi, Y.: Group Synchronization Control over Haptic Media in a Networked Real-Time Game with Collaborative Work, Netgames’06, Singapore (2006)Nunome, T., Tasaka, S.: Inter-destination synchronization quality in a multicast mobile ad hoc network. In: Proceedings of IEEE 16th International Symposium on Personal, Indoor and Mobile Radio Communications, pp. 1366–1370, Berlin, Germany (2005)Brandenburg, R., van Stokking, H., Van Deventer, M.O., Boronat, F., Montagud, M., Gross, K.: RTCP for inter-destination media synchronization, draft-brandenburg-avtcore-rtcp-for-idms-03.txt. In: IETF Audio/Video Transport Core Maintenance Working Group, Internet Draft, March 9 (2012)ETSI TS 181 016 V3.3.1 (2009-07) Telecommunications and Internet converged Services and Protocols for Advanced Networking (TISPAN); Service Layer Requirements to integrate NGN Services and IPTVETSI TS 182 027 V3.5.1 (2011-03) Telecommunications and Internet converged Services and Protocols for Advanced Networking (TISPAN); IPTV Architecture; IPTV functions supported by the IMS subsystemETSI TS 183 063 V3.5.2 (2011-03) Telecommunications and Internet converged Services and Protocols for Advanced Networking (TISPAN); IMS-based IPTV stage 3 specificationBrandenburg van, R., et al.: RTCP XR Block Type for inter-destination media synchronization, draft-brandenburg-avt-rtcp-for-idms-00.txt. In: IETF Audio/Video Transport Working Group, Internet Draft, Sept 24, 2010Williams, A., et al.: RTP Clock Source Signalling, draft-williams-avtcore-clksrc-00. In: IETF Audio/Video Transport Working Group, Internet Draft, February 28, 201

Subregional volume reduction of the cholinergic forebrain in subjective cognitive decline (SCD)

Subjective cognitive decline (SCD) patients are considered as a risk population for preclinical Alzheimer's Disease (AD). Supporting this idea, previous studies in SCD populations report subtle alterations in various cognitive and neuroimaging biomarkers that are typically affected during AD progression. To extend these observations, the present study examined whether SCD patients show atrophy of cholinergic basal forebrain nuclei (chBFN), analogous with recent findings in prodromal and clinical AD patients. We assessed volume reductions of the chBFN in 24 SCD subjects compared to 49 matched controls on 3D-T1-weighted MR images based on a postmortem derived atlas. Furthermore, we assessed whether chBFN atrophy was linked with cognitive, structural and metabolic biomarker alterations we previously reported in this SCD cohort: Using correlation analyses we tested for associations between the volumes of the chBFN with the hippocampal gray matter volume, and posterior medial glucose consumption, and the trajectory of verbal memory performance. The SCD cases showed a significant total volume reduction of the chBFN, with largest effect sizes in the Ch1/2 and Ch4p subdivisions of the chBFN. The latter was associated with a reduced glucose metabolism in the precuneus for the SCD group only. These data show an early involvement of the cholinergic basal forebrain nuclei in SCD predominantly in Ch1/2 and Ch4p which supports the conceptual link between SCD and preclinical AD

Vascularisation of ocular coralline hydroxyapatite implants

Vascularisation of coralline hydroxyapatite used to replace the enucleated bulb is of critical importance for the uncomplicated implantation of a motility peg connecting the implant with the cosmetic prosthesis. Technetium-99m diphosphopropanedicarboxylic acid (DPD) single-photon emission tomography (SPET) was used to evaluate the rate of vascularisation as well as the time required for completion of vascularisation. Twenty-four patients were enrolled in the study, which was designed to evaluate vascularisation 10 days, 2 months and 4 months after implantation of a coralline implant. Nineteen patients completed the study and the visual impression of the completion of the vascularisation was scored from 0 (no vascularisation) to ++++ (complete vascularisation) for each patient. No tracer accumulation was detected in any patient at the 10-day examination. Increasing vascularisation was demonstrated with time, and full vascularisation of the coralline implant was seen in all but one case by 4 months after implantation. We conclude that vascularisation of ocular coralline hydroxyapatite implants occurs early and is completed by 4 months after implantation in most cases, but should be confirmed at this time by99mTc-DPD SPET. © 1994, Springer-Verlag. All rights reserved

rCBF SPECT and the acetazolamide test in the evaluation of dementia

Background: The diagnostic potential of 99mTc-HMPAO following systemic administration of the cerebral vasodilator acetazolamide (acetazolamide test) was evaluated using regional-cerebral-blood-flow (rCBF) SPECT in patients with Alzheimer's disease (AD) or with vascular-type of dementia (VD). Methods: An initial, high-resolution SPELT study was performed with 99mTc-HMPAO, and after 2 days patients were re-evaluated with 99mTc-HMPAO following systemic administration of acetazolamide. Reconstructed SPELT slices were evaluated visually and semiquantitatively by a semiautomatic rCBF map method. Results: Using 99mTc-HMPAO alone, a bilateral hypoperfusion was found in the temporal and/or parietal regions in 33% (6/18) of VD patients and in 70% (23/33) of AD patients. The vascular reserve capacity, as determined with the acetazolamide test, was not impaired in 22% of the VD patients but in 76% of the AD patients. The differences in the perfusion patterns between VD and AD patients were statistically different (p < 0.01, Fischer's exact test). Of the 6 VD patients with hypoperfusion (bilateral temporal and/or parietal), 4 had a decreased vascular reserve capacity as determined in the acetazolamide test. Decreased reserve capacity was found in only 4 out of 25 patients with AD. Conclusions : The acetazolamide test is helpful in rCBF SPECT to differentiate VD from AD

Response and long-term control of bone metastases after peptide receptor radionuclide therapy with (177)Lu-octreotate

Peptide receptor radionuclide therapy (PRRT) is an efficient treatment for gastroenteropancreatic neuroendocrine tumors (GEP NETs), with outstanding overall response rates and survival. However, little is known about the particular efficacy regarding bone metastasis (BM)