Acceleration and vacuum temperature

The quantum fluctuations of an "accelerated" vacuum state, that is vacuum fluctuations in the presence of a constant electromagnetic field, can be described by the temperature \TEH. Considering \TEH for the gyromagnetic factor $g=1$ we show that \TEH(g=1)=\THU, where \THU is the Unruh temperature experienced by an accelerated observer. We conjecture that both particle production and nonlinear field effects inherent in the Unruh accelerated observer case are described by the case $g=1$ QED of strong fields. We present rates of particle production for $g=0,1,2$ and show that the case $g=1$ is experimentally distinguishable from $g=0,2$. Therefore, either accelerated observers are distinguishable from accelerated vacuum or there is unexpected modification of the theoretical framework.Comment: 4 pages, 1 figure; expanded discussion of experimental observables, added references, version appearing in Phys Rev

The BMV project: Search for photon oscillations into massive particles

In this contribution to PSAS08 we report on the research activities developed in our Toulouse group, in the framework of the BMV project, concerning the search for photon oscillations into massive particles, such as axion-like particles in the presence of a strong transverse magnetic field. We recall our main result obtained in collaboration with LULI at \'Ecole Polytechnique (Palaiseau, France). We also present the very preliminary results obtained with the BMV experiment which is set up at LNCMP (Toulouse, France).Comment: Proceedings of PSAS'08, to be published in Can. J. Phy

Detection and identification methods and new tests as developed and used in the framework of cost 873 for bacteria pathogenic to stone fruits and nuts

Xanthomonas arboricola pv. pruni (Xap), the causal agent of bacterial spot disease of stone fruits and almond, is regulated as a quarantine pathogen in the European Union and the European and Mediterranean Plant Protection Organization (EPPO). Xap can have an epiphytic phase and/or be latent and, consequently, it can be transmitted by different types of plant material. Effective quarantine measures require specific, sensitive and rapid methods to detect Xap in propagative material or new reservoirs. Laborious and time-consuming methods for the diagnosis of Xap are recommended in the current EPPO standard protocol. However, new several pathogen-specific PCR and quantitative realtime PCR assays have been developed that enable direct detection of Xap in symptomatic and symptomless plant samples. A concise resource of current methods for Xap detection and identification, based on assessment and development activities within the framework of COST 873, is presented

Gene expression analysis in cold stress conditions reveals BBX20 and CLO as potential biomarkers for cold tolerance in almond

Late spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [Prunus amygdalus Batsch syn P. dulcis (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order to identify key genes favoring cold tolerance in almonds, branches from three late-blooming genotypes: ‘Guara’, ‘Soleta’ and ‘Belona’ were exposed at -4¿C during 24 h in a constant climate chamber. Phenotype analysis showed that ‘Guara’ and ‘Soleta’ had a greater acclimation capacity to cold than ‘Belona’. The qRT-PCR BioMark System technology was used to monitor the relative expression of 30 candidate genes with a potential relation to cold response, which are either involved in the ICE-CBF-COR pathway or the independent CBF pathway, and also genes not yet characterized or with unknown function in almond genome. Differences in the gene expression profiles were found among the three studied genotypes and the three time-points of cold exposure (0, 2 and 24 h). BBX20 and CLO genes behaved as differentiator genes between tolerant and susceptible genotypes in cold stress response in almond pistils. In addition, the differences of expression among the tolerant genotypes suggested the intervention of different mechanisms responding to cold stress in almonds. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Expresión de genes relacionados con la respuesta a estrés hídrico en frutales de hueso (Prunus spp)

Los frutales de hueso (Prunus spp.) difieren en cuanto a su comportamiento frente a determinados estreses medioambientales. En este trabajo se ha estudiado la respuesta a la sequía en dos genotipos utilizados como portainjertos en distintas condiciones de suelos. El híbrido almendro x melocotonero (P. amygdalus x P. persica) ‘Garnem’ y su descendiente el trihíbrido ‘P.2175’ x ‘Garnem’-3 (P. cerasifera x [P. amygdalus x P. persica]). Ambos se sometieron a un periodo de déficit hídrico durante un mes, seguido de un periodo de recuperación en umbráculo. El tratamiento de estrés se realizó manteniendo las plantas a un contenido de humedad del sustrato del 35%. Se tomaron datos fisiológicos a los 0, 10 y 15d de estrés y a los 10d y 15d de recuperación. Igualmente se estudio la expresión de dos genes en dos tejidos diferentes, que codifican para proteínas relacionadas con el ABA y el estrés abiótico, una dehidrina y una proteína de unión a zinc respectivamente. Los parámetros fisiológicos de conductancia estomática y de potencial hídrico fueron monitorizados y correlacionados con la expresión génica. Se observó que existe una relación inversa entre los parámetros fisiológicos y moleculares. La relación de ambos parámetros y su implicación en la mejora de la tolerancia a este estrés será discutida

Caracterización de la respuesta adaptativa a sequía de dos cultivares de melocotonero en condiciones de aridez

Se evaluó la respuesta a sequía de dos cultivares de melocotonero con distinta época de maduración, 'Ufo-3' y 'Fergold', injertados sobre el patrón híbrido almendro × melocotonero, 'Garnem', en los veranos de 2015 y 2016 en Murcia, España. En poscosecha, los árboles se sometieron a 5 días sin riego y, posteriormente, a 5 días de recuperación de riego. Se evaluó el estado hídrico del árbol, el intercambio gaseoso, así como el contenido en ácido abscísico (ABA) foliar. Se comprobó un descenso del contenido hídrico del suelo (CHS), que ocasionó la reducción del potencial hídrico foliar (PHF) y el cierre estomático. Reiniciado el riego, el estado hídrico de los árboles estresados fue recuperado. Se observó una correlación negativa entre el PHF y el uso eficiente del agua intrínseco (UEAi) en 2015, indicando una posible estrategia de tolerancia mediante la cual se conservase la capacidad fotosintética, a pesar de los bajos valores de conductancia estomática (gs) y PHF. Por otra parte, la regulación del cierre estomático observada en los árboles estresados no fue provocada por la acumulación de ABA. Esta evaluación agronómica permitirá una mayor comprensión del posible efecto del patrón 'Garnem' en la adaptación fisiológica y bioquímica a la sequía y su comportamiento en recuperación bajo condiciones de campo áridas de los cultivares de melocotonero injertados

Graft Risk Index After Liver Transplant: Internal and External Validation of a New Spanish Indicator

OBJECTIVES: Scarcity of liver grafts has led to the use of marginal donors, consequently increasing the number of complications posttransplant. To prevent this situation, several indicators have been developed. However, important differences remain among countries. Here, we compared an early-risk liver transplant indicator based on the Spanish Liver Transplant Registry, called the Graft Risk Index, versus the US donor risk index and the Eurotransplant donor risk index. MATERIALS AND METHODS: The new indicator was based on prospectively collected data from 600 adult liver transplants performed in our center. We considered 2 events to compare the indexes: graft survival and rejection-free graft survival, with Cox proportional regression for analyses. Power to predict graft survival was evaluated by calculating the receiver operating characteristic area under the curve. RESULTS: We found no differences between the US and Eurotransplant donor risk indexes in prediction of patients with and without early graft failure. With regard to early survival, only the Graft Risk Index allowed better survival discrimination, in which survival progressively decreased with values = 3 (with probability of graft survival at 1 month of 68%; 95% confidence interval, 46.2-82.5). This increase in risk was significant compared with the standard group (hazard ratio of 10.15; 95% confidence interval, C 3.91- 26.32; P < .001). We calculated powers of prediction of 0.52 (95% confidence interval, 0.43-0.62), 0.54 (95% confidence interval, 0.45-0.65), and 0.69 (95% confidence interval, 0.61-0.77) for donor risk index, Eurotransplant donor risk index, and early Graft Risk Index, respectively. CONCLUSIONS: Neither the US donor risk index nor the Eurotransplant donor risk index was valid for our Spanish liver donation and transplant program. Therefore, an indicator to predict posttransplant graft survival that is adapted to our environment is necessary. This national Graft Risk Index can be a useful tool to optimize donor-recipient matchin

The BMV experiment : a novel apparatus to study the propagation of light in a transverse magnetic field

In this paper, we describe in detail the BMV (Bir\'efringence Magn\'etique du Vide) experiment, a novel apparatus to study the propagation of light in a transverse magnetic field. It is based on a very high finesse Fabry-Perot cavity and on pulsed magnets specially designed for this purpose. We justify our technical choices and we present the current status and perspectives.Comment: To be published in the European Physical Journal

Phytochemical Screening and Antibacterial Activity of Taxus baccata L. against Pectobacterium spp. and Dickeya chrysanthemi

The yew tree (Taxus baccata L.) is considered in folklore a symbol of immortality due to its qualities of longevity and regeneration. Despite its poisonous reputation, the yew tree has a long history of medicinal use, particularly in the form of extracts from its leaves and bark. In the work presented herein, gas chromatography–mass spectrometry (GC–MS) chemical profiling was applied to the aqueous ammonia/hydromethanolic extracts of several plant organs of T. baccata, leading to the identification of different bioactive compounds than those previously characterized by high-performance liquid chromatography with tandem mass spectrometry (HPLC–MS/MS) in other extraction media. The leaf aqueous ammonia extract was rich in 2-hexylthiophene and 3-O-methyl-d-fructose; 9-octadecenoic and hexadecanoic acid were the main constituents of the bark aqueous ammonia extract; and the fruit hydromethanolic extract contained methyl 2-O-methyl-α-d-xylofuranoside, 1,3-dioxolane derivatives, and erysimoside. The antimicrobial activity of the extracts was assayed against four bacterial pathogens responsible for the soft rot and blackleg diseases of potatoes, viz. Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum, Pectobacterium parmentieri, and Dickeya chrysanthemi, resulting in minimum inhibitory concentration (MIC) values as low as 187 μg·mL−1. Bioassays on potato slices confirmed the efficacy of the leaf extract at this dose when applied as a preventive treatment before artificial inoculation with P. carotovorum subsp. carotovorum. In view of this high activity, these extracts may find application in the integrated pest management of soft rot Pectobacteriaceae (SRP) diseases.Publishe

Supervivencia del injerto tras trasplante hepático: aproximación a un nuevo índice de riesgo español

Introducción: existen diversos indicadores para la valoración de la supervivencia del injerto hepático (DRI americano y ET-DRI europeo, entre otros), pero existen diferencias importantes entre los programas de trasplante de los diferentes países y podría ser que dichos indicadores no sean válidos en nuestro medio. Objetivos: el objetivo de este estudio es describir un nuevo indicador nacional de riesgo del injerto hepático a partir de los resultados del Registro Español de Trasplante Hepático (RETH) y validar el DRI y el ET-DRI. Metodología: el RETH incluye un análisis de Cox de los factores relacionados con la supervivencia del injerto. En base a sus resultados se define el indicador graft risk index (GRI). Las variables que contempla dependen del proceso de donación: edad, causa de muerte, compatibilidad sanguínea y tiempo de isquemia fría; y del receptor: edad, enfermedad de base, virus C, número de trasplante, estado UNOS y técnica quirúrgica. Se obtuvo la curva de la regresión logística y se calcularon las curvas de supervivencia del injerto por estratificación. La precisión se evaluó mediante el área ROC. Resultados: un GRI de 1 se corresponde con una probabilidad de pérdida del injerto del 23, 25%; cada punto de aumento del GRI supone que la probabilidad se multiplica por 1, 33. El GRI mostró la mejor discriminación por estratificación. El área ROC del DRI fue 0, 54 (95% IC, 0, 50-0, 59) y del ET-DRI, 0, 56 (95% IC, 0, 51-0, 61), frente al GRI 0, 70 (95% IC, 0, 65-0, 73) (p < 0, 0001). Conclusiones: el DRI y el ET-DRI no parecen útiles en nuestro medio y sería necesario disponer de un indicador propio. El GRI requiere un estudio nacional que perfile más el indicador y realice una validación más amplia. Introduction: several indicators are available to assess liver graft survival, including the American DRI and the European ET-DRI. However, there are significant differences between transplant programs of different countries, and the previously mentioned indicators might be not valid in our setting. Objectives: the aim of the study was to describe a new national liver graft risk indicator based on the results obtained from the Registro Espanol de Trasplante Hepatico (RETH) and to validate the DRI and ET-DRI indicators. Methods: the RETH includes a Cox analysis of factors associated with graft survival; the graft risk index (GRI) indicator was defined based on these results. The variables considered are dependent upon the donation conditions (age, cause of death, blood compatibility and cold ischemia time) and the transplant recipient (age, underlying disease, hepatitis C virus, transplant number, UNOS status and surgical technique). A logistic regression curve was obtained and graft survival curves were calculated by stratification. Precision was assessed using the ROC analysis. Results: a GRI of 1 represents a probability of graft loss of 23.25%; each point increase in the GRI score multiplies this probability by 1.33. The best discrimination of GRI was obtained by stratification.The DRI ROC area was 0.54 (95% CI, 0.50-0.59) and the ET-DRI ROC area was 0.56 (95% CI, 0.51-0.61), compared to 0.70 (95% CI, 0.65-0.73) (p < 0.0001) for the GRI. Conclusions: both the DRI and ET-DRI do not seem to be useful in our setting. Hence a national indicator is more desirable.The GRI requires a national study in order to further streamline and assess this indicator