PRELIMINARY PHYTOCHEMICAL SCREENING AND TO EVALUATE ANTI-OXIDANT PROPERTY ON ROOT EXTRACT OF DILLENIA INDICA (ELEPHANT APPLE)

Objective: The objective of the present study was to carry out the presence of antioxidants activity on root extract of Dilleniaindica (Family-Dilleniaceae) which is believed to have the protective mechanisms in beneficial health effects. Methods: Considering its medicinal importance, the plant was chosen for extraction with various solvents such as petroleum ether, chloroform and ethanol which was taken into considerations to determine the phytochemicals analyses present in it. The extracts of the roots were evaluated for antioxidant activity by using different in vitro models like Reducing Power method, TBA Method and DPPH method at different doses (20,50,100,200,400μg/ml). The ic50 values of each extract on different activity were carried out. Results: The study shows that petroleum ether, chloroform and ethanolic extract of this plant showed potent antioxidant activity against the standard drug (Ascorbic acid). But chloroform extract of the roots shown most significant anti-oxidant activity as compared to petroleum ether and ethanol. Conclusion: The root part of the plant shows active anti-oxidant activity that can be consumed by mankind

Recombinant Protein Production: Advancements And Applications

Recombinant gene production has become a cornerstone of modern biotechnology, offering revolutionary solutions in various fields. This abstract explores the diverse applications of recombinant gene technology, highlighting its pivotal role in medicine, agriculture and industry, while also discussing the latest advancements and challenge. Recombinant gene production involves the deliberate manipulation of genetic material to create hybrid DNA sequences with desired traits. This technology has enabled the synthesis of therapeutic proteins, vaccines and enzymes that were once scarce or inaccessible. In medicine recombinant gene expression systems have revolutionized the treatment of various diseases, ranging from insulin production for diabetesb management to monoclonal antibody therapies for cancer treatment. In agriculture, recombinant gene technology has been harnessed to develop genetically modified crops with enhanced nutritional content, resistance to pests and diseases and improve yield. These advancements hold promise for addressing global food security challenges, although they also raise concerns about ecological impact and consumeracceptance. Industrial applications of recombinant gene production encompass the production of biofuels, bioplastics and wide array of biobased chemicals. This technology enables the cost effective production of valuable compounds through microbial fermentation or other bioprocesses, contributing to sustainable manufacturing practices. Recent advancements in recombinant gene production include the development of novel expression systems such as synthetic biology tools that enable precise control over gene expression and metabolic pathways. Additionally, innovations like CRISPR based genome editing have accelerated the modification of host organisms for improved recombinant protein yields and functionalities. Despite its transformative potential, recombinant gene production faces challenges. Ensuring the safety and regulatory compliance of genetically modified products remain a priority. Furthermore, optimising expression systems to maximize yields, scalability, and post translational modifications is ongoing endeavou

Recombinant Hormones: Applications And Challenges

Recombinant DNA technology has brought about a revolutionary transformation in the production of medical hormones. This comprehensive review paper presents an overview of the fundamental principles underlying recombinant DNA technology and its significant applications in hormone production. The discussion encompasses the production of various recombinant hormones, including human growth hormone, infertility treatment hormones, menopause and osteoporosis control hormones, and insulin. The paper delves into the challenges associated with recombinant hormone production, emphasizing the importance of meticulous optimization of expression systems and addressing potential contamination concerns. Furthermore, the review explores the utilization of recombinant hormones in medical research, contributing to the development of diagnostic and therapeutic protocols. Ethical considerations and safety concerns related to the use of recombinant hormones are also addressed, emphasizing the imperative need for stringent regulation in this rapidly advancing field. This paper provides a comprehensive and plagiarism-free exploration of the multifaceted aspects of recombinant DNA technology and its pivotal role in advancing hormone production for medical application

CrysXPP: An explainable property predictor for crystalline materials

We present a deep-learning framework, CrysXPP, to allow rapid and accurate prediction of electronic, magnetic, and elastic properties of a wide range of materials. CrysXPP lowers the need for large property tagged datasets by intelligently designing an autoencoder, CrysAE. The important structural and chemical properties captured by CrysAE from a large amount of available crystal graphs data helped in achieving low prediction errors. Moreover, we design a feature selector that helps to interpret the model’s prediction. Most notably, when given a small amount of experimental data, CrysXPP is consistently able to outperform conventional DFT. A detailed ablation study establishes the importance of different design steps. We release the large pre-trained model CrysAE. We believe by fine-tuning the model with a small amount of property-tagged data, researchers can achieve superior performance on various applications with a restricted data source

THE ANTIDEPRESSANT DRUG DOXEPIN: A PROMISING ANTIOXIDANT

ABSTRACTObjective: Oxidative stress contributes to the pathophysiology of exposure to environmental pollutants and to different free radical generatingbiochemical reactions in a human system leading to various types of age-related ailments. An antioxidant is a substance capable of preventingor slowing down oxidation of other molecules. Administration of different natural or synthetic antioxidants can ably lead to prevention andattenuation of such stress-induced biochemical alterations. Best examples of natural antioxidants are plant-derived flavonoids. The presentstudy has been designated to determine antioxidant properties in antidepressant compound doxepin which is structurally similar to flavonoidquercetin.Methods: Antioxidant capacity in doxepin was determined with help of several standard conventional procedures such as phosphomolybdenumassay, Ferric ion and cupric ion reducing power assays, ferrous ion chelating activity assay, hydrogen peroxide, and nitric oxide scavenging activityassays.Results: Doxepin showed a stable rise in its antioxidant activity in a dose-dependent manner as determined by ferric as well as cupric ion reducingcapacity and by phosphomolybdenum assay. An almost identical observation was noted while determining ferrous ion chelating activity. Furthermore,doxepin showed a strong nitric oxide scavenging activity in all the concentrations used in the study while its hydrogen peroxide scavenging activitywas only observed at 500 μg/ml of doxepin.Conclusion: Thus, our study opens up a new vista in search for antioxidants not only from plant sources but also from clinically establishedpharmaceutical compounds that are already in practical use among patients.Keywords: Antioxidant, Antidepressant, Doxepin, Quercetin, Flavanol, Flavonoids

Revolutionizing the Biological Landscape: the Power of Genome Editing

In recent decades, the advent of genome editing has brought about profound transformations in biology, allowing for precise modifications to the genetic material of living organisms beyond traditional genetic manipulation methods. This summary explores the vast potential, diverse applications, and ethical considerations associated with genome editing. Led by CRISPR-Cas9, this technology revolutionizes genetic engineering by providing unparalleled accuracy and versatility. Scientists can now manipulate genes with unprecedented precision, impacting various fields such as agriculture and medicine. Genome editing facilitates the creation of genetically modified organisms with desirable traits, from enhancing crop disease resistance to pioneering human therapies. Moreover, it sheds light on gene function, offering crucial biological insights. Despite its transformative potential, ethical concerns accompany genome editing, especially in terms of editing the germ line and its implications for future generations. This necessitates ongoing discussions to address unintended consequences, highlighting the importance of responsible use. Ultimately, genome editing represents a revolutionary advancement with the potential to transform agriculture, medicine, and our understanding of life. Moving forward, inclusive dialogues involving scientists, ethicists, policymakers, and the public are essential to ensure the responsible application of genome editing for the benefit of humanity and the environment

Factors for COVID-19 Infection that Govern the Severity of Illness

Coronaviruses have been posing a serious threat to mammals and birds and a new class of SARS-CoV is creating havoc to the world after its first incidence in Wuhan City in China in December 2019. These viruses are mainly responsible for causing serious respiratory tract infections which generally appear initially as the common cold and can be lethal just like SARS-CoV. The problems seem to vary and worsen from one person to another depending on age, gender, ethnicity, blood groups, host genetics, and associated comorbidities. Complications should also arise as this virus keeps mutating and evolving. This review points out the various underlying causes behind the severity of the illness and the mechanisms associated with it. This review will help society to understand the risks and severities associated with COVID-19. Individuals with health complexities and predispositions listed in this review are the most vulnerable in terms of severity and should take every possible measure to protect themselves from getting infected. As a consequence, this will lead to a decrease in mortality rates arising from COVID-19.   Doi: 10.28991/SciMedJ-2021-0302-9 Full Text: PD

Microbial Plastic Degradation: Nature's Solution for Sustainable Waste Management

Plastic pollution has emerged as a global environmental crisis, demanding innovative and sustainable waste management strategies. This review explores the potential of harnessing nature's capabilities, specifically through microbial plastic degradation, as a promising avenue for sustainable waste management. The focus is on the collaborative action of microorganisms, utilizing their enzymatic activities to enhance plastic degradation. This review delves into the intricate mechanisms of microbial interaction with various types of plastics, emphasizing recent advancements in microbial plastic degradation research. Furthermore, it discusses the challenges associated with scaling up microbial degradation processes and envisions the incorporation of these approaches into practical waste management solutions. This exploration of microbial plastic degradation represents a critical step in mitigating the environmental impact of plastic pollution and promoting a more sustainable and eco-friendly waste management paradigm

Prevalence and characteristics of polycystic ovarian syndrome in women attending in outpatient department of obstetrics and gynecology of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

Background: Polycystic ovary syndrome (PCOS) is a heterogenous, multifactorial, complex genetic disorder. Most commonly, it affects the females of reproductive age. This is one of the most widespread diseases across the world and if left untreated, may result in infertility and even uterine cancer. Methods: A cross sectional observation study of 100 PCOS patients was carried out from August 2018 to July 2019 in gynecology out-patient department of Bangabandhu Sheikh Mujib medical university, Dhaka. In this study clinical, biochemical and hormonal profile of these patients were analyzed and correlation was done between clinical features and biochemical and hormonal profile. Results: The prevalence of PCOS was 6.11% in the gynecology out-patient visits and 35.39% among infertile women. The mean age group of the patients was 24.3±5.16 SD. The mean BMI was 24.66±5.34 SD. The mean duration of infertility was 5.17 years. The prevalence of metabolic syndrome in our study was 15.0%. In this study menstrual irregularity was the most common complaint. Spearman’s correlation between various clinical and laboratory parameters showed positive correlation exists between BMI and testosterone (r=0.4824; p<0.0001).Conclusions: The study showed that most of our polycystic ovary syndrome subjects were present with oligomenorrhea. Hirsutism and central obesity were also common presentation. Obese women with PCOS had more severe ovulatory dysfunction and need more attention for their appropriate management.