Multidisciplinary approach in breast cancer.

Breast cancer is the most common neoplasm among women. Worldwide, there will be about 2.1 million newly diagnosed female breast cancer cases in 2018, accounting for almost 1 in 4 cancer cases among women. The disease is the most frequently diagnosed cancer in the vast majority of the countries. The purpose of this article is to report the positive experience of a multidisciplinary team in the care of women with breast cancer and their family members. Our approach that is part of the Cancer Patient Support Center (CPSC) at the public health (IPSEMG) in Brazil has been developed taking into account a broader concept of health care. We value not only individual dimensions in patient care, but also common ones, we recognize the importance of dealing with non-biological aspects of the disease, such as socioeconomic, political and cultural facets and our service is focused on health promotion rather than merely on curative treatment. Among the advantages of our approach, we highlight the facilitated accessibility to health services, the patient-centered communication and shared decision making, and the strong bonds between health professionals, patients, and family members. As part of CPSC`s activities, we emphasize the services provided by ?Aconchego? (?Warmth?), that is our breast cancer support group at public health in Brazil

Uso da citometria de fluxo para detecção de Babesia canis

Exportado OPUSMade available in DSpace on 2019-08-14T03:11:21Z (GMT). No. of bitstreams: 1 disserta__o_de_mestrado_de_kelly_alves_bicalho.pdf: 14909808 bytes, checksum: b2d2c5e8a7b6f2a949f54a210e1b616b (MD5) Previous issue date: 1A técnica de citometria de fluxo foi avaliada para detecção de hemácias parasitadas com Banesia canis. Na padronização da técnica foram testados lodeto de propídeo e Hidroetidina, como marcadores das hemácias parasitadas. Hidroetidina apresentou melhores resultados e foi utilizada nos demais experimentos. Cães controle e experimentalmente infectados com B. canis foram acompanhados por citometria de fluxo e esfregaços sanguíneos, capilar e venoso, por períodos de 21 a 195 dias. No esfregaço sanguíneo as primeiras hemácias parasitadas foram detectadas no segundo dia após inoculação, com pico de parasitemia capilar e venosa ocorrendo na primeira semana. Após este período, a presença do parasito passou a ser um achado inconstante, com eventuais períodos de ausência, que não obedeceram a um padrão de comportamento.The technique of flow cytometry was evaluated for detection of infected erythrocytes with Banesa kennels. In the standardization of the technique have been tested and propidium lodeto Hidroetidina as markers of infected erythrocytes. Hidroetidina showed better results and was used in other experiments. Control dogs and experimentally infected with B. canis were followed by flow cytometry and blood smears, capillary and venous blood, for periods from 21 to 195 days. In smears the first infected erythrocytes were detected on the second day after inoculation, with peak parasitemia capillary and venous occurring during the first week. After this period, the presence of the parasite has become an inconsistent finding, with occasional periods of absence, have not obeyed a pattern of behavior

Condições higiênico-sanitárias, ocorrência de parvovírus e de parasitos de roedores em colônias de camundongos e ratos de biotérios brasileiros

Submitted by Nuzia Santos ([email protected]) on 2012-08-22T14:05:33Z No. of bitstreams: 1 Tese_Kelly Alves Bicalho.pdf: 6753401 bytes, checksum: b6563a50303d196054fe69f7c38308d6 (MD5)Made available in DSpace on 2012-08-22T14:05:33Z (GMT). No. of bitstreams: 1 Tese_Kelly Alves Bicalho.pdf: 6753401 bytes, checksum: b6563a50303d196054fe69f7c38308d6 (MD5)Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou Fundação de Amparo à Pesquisa de Minas Gerais. Conselho Nacional de Desenvolvimento Científico e TecnológicoFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.As condições sanitárias de 17 biotérios de instituições públicas de ensino e/ou pesquisa, produção de produtos farmacêuticos e controle de qualidade de imunobiológicos de diferentes regiões geográficas do Brasil e a ocorrência de parvovírus e de parasitos de roedores em colônias de camundongos e ratos foram avaliadas. Dados sobre barreiras sanitárias para evitar a transmissão de doenças e de programa de monitoramento de saúde animal foram obtidos através da aplicação de um questionário. Métodos sorológicos (IFI e IHA) e PCR foram utilizados para diagnóstico de parvovírus em 563 camundongos e 167 ratos. Métodos parasitológicos foram utilizados para o diagnóstico de ácaros, piolhos, helmintos e protozoários em 611 camundongos e 183 ratos. A maioria dos biotérios avaliados não possui instalações e barreiras sanitárias de proteção apropriadas para evitar a transmissão de infecções e infestações por patógenos. Maior positividade de infecção por parvovírus foi detectada pela técnica de PCR. Nas 563 amostras de camundongos a ocorrência de parvovírus por métodos sorológicos foi de 18,3% (MVM - 6,2%; MPV - 12,3%) e a positividade variou de 0,0% a 22,5% nas diferentes regiões geográficas; por PCR foi de: 49,2% (MVM - 12,3%; MPV - 43,5%) e a positividade variou de 16,7% a 100%. Ns 167 amostras de rato a ocorrência de parvovírus por métodos sorológicos foi de: 40,7% (H-1 - 1,8%; KRV - 3,0%; RPV-1/RMV-1 - 35,9%) e amostras positivas foram dectadas somente na região SE; por PCR foi de: 73,7% (H-1 - 0%; KRV - 6,0%; RMV-1 - 37,7%; RPV-1 - 54,5%) e a positividade vairou de 25,0% a 100,0%. MPV e RPV-1 foram os vírus mais freqüentes e detectados em todas as regiões avaliadas. Biotérios com menor número de barreiras sanitárias (Categoria C) apresentaram maior ocorrência de parvovírus. Análises de concordância demonstraram não haver concordância ou concordância fraca (K=0,036 a 0,514) entre os métodos sorológicos e a PCR para detecção de infecção por parvovírus. Na região SE, parvovírus foram detectados por PCR em biotérios dos Estados de São Paulo, Rio de Janeiro e Minas Gerais. Em nove instituições públicas do Estado de Minas Gerais foi observada elevada ocorrência de infecção por parvovírus (35% a 100%), sendo detectadas co-infecções por MVM e MPV em seis biotérios (75%) e por RPV e RMV em cinco biotérios (71,4%). Alta ocorrência de parasitas foi observada nos biotérios avaliados, sendo Shypacia spp., Spironucleus muris, Tritrichomonas muris, Trichomonas minuta e Entamoeba muris os mais frequentes nas colônias de camundongos e ratos.The sanitary conditions of 17 animal facilities in public education / research, pharmaceutical production and quality control of immunobiological from different geographic regions of Brazil and the occurrence of of rodent parvoviruses and parasites in mouse and rat colonies were evaluated. Data on sanitary barriers to prevent transmission of diseases and health monitoring program were obtained through the application of a standardized questionnaire. Serological methods (IFA and IHA) and PCR were used for diagnosis of parvoviruses in 563 mice and 167 rats. Parasitological methods were used for diagnosis of mites, lice, helminthes and protozoa in 611 mice and 183 rats. Most of the animal facilities evaluated do not present appropriate protective barriers to prevent transmission of infections and infestations by pathogens. Greater number of positive rodent parvoviruses infection was detected by PCR. Of the 563 mice samples tested, the occurrence of parvoviruses using serology was 18.3% (MVM - 6.2%; MPV - 12.3%) and the positivity ranged from 0.0 to 22.5% in different geographic regions; by PCR was: 49.2% (MVM - 12.3%; MPV - 43.5%) and positivity ranged from 16.7 to 100.0%. Of the 167 rat samples tested, the occurrence of rat parvoviruses by serological methods was: 40.7% (H-1 - 1.8%; KRV - 3.0%; RPV-1/RMV-1 - 35.9%) and positive samples were detected only in the Southeast region (51.5%); by PCR was: 73.7% (H-1 - 0%; KRV - 6.0%; RMV-1 - 37.7%; RPV-1 - 54.5%) and positivity ranged from 25.0 to 100.0%. MPV and RPV-1 viruses were detected more frequently and in all regions evaluated. Animal facilities with fewer sanitary barriers (Category C) had increased occurrence of parvoviruses. Analysis of agreement showed no correlation or weak correlation (K = 0.036 to 0.514) between PCR and serological methods for detection of rodent parvoviruses infection. In the SE region, parvoviruses were detected by PCR in animal facilities in the State of São Paulo, Rio de Janeiro and Minas Gerais. In nine public institutions of the State of Minas Gerais was observed high occurrence of parvoviruses infection (35% to 100%) being detected co-infections with MVM and MPV in six animal facilities (75 %) and with RPV and RMV in five animal facilities (71.4%). High occurrence of parasites was observed in the animal facilities evaluated, and Shypacia spp. Spironucleus muris, Tritrichomonas muris, Trichomonas minuta and Entamoeba muris were the most frequent in the mouse and rat colonies

Quality perception in research laboratories from Fiocruz after QMS implementation

Quality Management System has been implemented at the René Rachou Research Center since 2003. This study investigated its importance for collaborators (Cs) in laboratories. This was a quantitative and descriptive study performed in a group of 113 collaborators. It was based on the World Health Organization handbook: Quality Practices in Basic Biomedical Research. The questionnaires evaluated the parameters using the Likert scale. Biosafety, training and ethics were considered to be the most important parameters. Supervision and quality assurance, data recording, study plan, SOPs and file storage achieved intermediate evaluation. The lower frequency of responses was obtained for result report, result verification, personnel and publishing practices. Understanding the perception of the collaborators allows the development of improvement actions aiming the construction of a training program directing strategies for disseminating quality

Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment.

BackgroundIn view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected with Schistosoma mansoni after Praziquantel (PZQ) treatment.Methodology/prinicpal findingsC57BL/6 mice infected with S. mansoni were treated with PZQ and transplanted intravenously with ASC from uninfected mice. Liver morpho-physiological and immunological analyses were performed. The combined PZQ/ASC therapy significantly reduced the volume of hepatic granulomas, as well as liver damage as measured by ALT levels. We also observed that ASC accelerated the progression of the granulomatous inflammation to the advanced/curative phase. The faster healing interfered with the expression of CD28 and CTLA-4 molecules in CD4+ T lymphocytes, and the levels of IL-10 and IL-17 cytokines, mainly in the livers of PZQ/ASC-treated mice.ConclusionsOur results show that ASC therapy after PZQ treatment results in smaller granulomas with little tissue damage, suggesting the potential of ASC for the development of novel therapeutic approaches to minimize hepatic lesions as well as a granulomatous reaction following S. mansoni infection. Further studies using the chronic model of schistosomiasis are required to corroborate the therapeutic use of ASC for schistosomiasis

Image_1_A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection.tif

In the present work, we developed and evaluated the performance of a new flow cytometry-based single platform, referred to as “FC-Duplex IgG1 (HTLV-1/2)”, for universal and differential serodiagnosis of HTLV-1/2 infection. The proposed technology employs a system for detection of IgG1 antibodies in a single competitive immunofluorescence platform by flow cytometry using fluorescently labeled MT-2/MoT cell line mix coupled to a highly sensitive development system (Biotin/Streptavidin/Phycoerythrin). The stability of fluorescent labeling and the antigenicity of MT-2 and MoT cell lines were confirmed upon storage at −20°C for 2, 6, and 12 months. The anti-HTLV-1/2 IgG1 reactivity, expressed as percentage of positive fluorescent cells (PPFC), was evaluated for each target antigen along the titration curve of test serum samples (1:32 to 1:4,096). Upon selection of target cell line and serum dilutions with higher segregation score between groups, the performance of “FIX” and “FIX & PERM” protocols was evaluated. The “FIX” protocol presented excellent performance indices (Se = 92%/Sp = 94%/AUC = 0.96; Se = 96%/Sp = 100%/AUC = 0.99) for the universal (HTLV-1/2 vs. NI) and differential (HTLV-1 vs. HTLV-2) diagnosis of HTLV-1 infection, respectively. Optimization of the “FIX” protocol using the principle of synchronous and asynchronous pairwise analysis further improved the performance of “FC-Duplex IgG1 (HTLV-1/2)”, using the “FIX” protocol for differential diagnosis of HTLV-1 and HTLV-2 infections (Se = 100%/Sp = 100%/AUC = 1.00). In conclusion, the “FC-Duplex IgG1 (HTLV-1/2)” method represents an innovation in the biotechnology segment with the potential to compose a serological kit for differential diagnosis of HTLV-1/2 infection for reference laboratories and blood centers.</p

Serum soluble mediator profiles and networks during acute infection with distinct DENV serotypes

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq. Funding was also obtained from Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM/PPP-CNPq, EDITAL N. 016/2014), Ministerio da Saude do Brasil (Chamada Publica no 01/2012, Convenio # 776823/2012) and INCT para Febres Hemorragicas Virais (INCT-FHV - 573739/2008-0).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilU.S. Food and Drug Administration. Center for Biologics Evaluation and Research. Office of Blood Research and Review. Silver Spring, MD, United States.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. Rio de Janeiro, RJ, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brazil / Universidade Federal do Amazonas. Escola de Enfermagem de Manaus. Manaus, AM, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brazil / Universidade Federal do Amazonas. Escola de Enfermagem de Manaus. Manaus, AM, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, BrazilUniversidade Federal do Amazonas. Escola de Enfermagem de Manaus. Manaus, AM, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brazil / Universidade Federal do Amazonas. Escola de Enfermagem de Manaus. Manaus, AM, Brazil / Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas. Diretoria de Ensino e Pesquisa. Manaus, AM, BrazilUniversidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrazilUniversidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.A panoramic analysis of chemokines, pro-inflammatory/regulatory cytokines, and growth factors was performed in serum samples from patients with acute DENV infection (n=317) by a high-throughput microbeads array. Most soluble mediators analyzed were increased in DENV patients regardless of the DENV serotype. The substantial increase (>= 10-fold) of CXCL10, IL-6, and IFN-gamma, and decreased levels of PDGF (= 3-9-fold) were selectively observed in DENV2 as compared to DENV1 and DENV4. Heatmap and biomarker signatures further illustrated the massive release of soluble mediators observed in DENV patients, confirming the marked increase of several soluble mediators in DENV2. Integrative correlation matrices and networks showed that DENV infection exhibited higher connectivity among soluble mediators. Of note, DENV2 displayed a more complex network, with higher connectivity involving a higher number of soluble mediators. The timeline kinetics (Day 0-1, D2, D3, D4-6) analysis additionally demonstrated differences among DENV serotypes. While DENV1 triggers a progressive increase of soluble mediators towards D3 and with a decline at D4-6, DENV2 and DENV4 develop with a progressive increase towards D4-6 with an early plateau observed in DENV4. Overall, our results provided a comprehensive overview of the immune response elicited by DENV infection, revealing that infection with distinct DENV serotypes causes distinct profiles, rhythms, and dynamic network connectivity of soluble mediators. Altogether, these findings may provide novel insights to understand the pathogenesis of acute infection with distinct DENV serotypes

Serotype-associated immune response and network immunoclusters in children and adults during acute Dengue virus infection

Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.U.S. Food and Drug Administration. Center for Biologics Evaluation and Research. Office of Blood Research and Review. Silver Spring, MD, USA.U.S. Food and Drug Administration. Center for Biologics Evaluation and Research. Office of Blood Research and Review. Silver Spring, MD, USA.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade Federal do Amazonas. Manaus, AM, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.Universidade Federal do Amazonas. Manaus, AM, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade Federal do Amazonas. Manaus, AM, Brazil / Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brazil.Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Campus Patos de Minas, MG, Brazil / Universidade Federal de Uberlândia. Faculdade de Engenharia Elétrica. Laboratório de Tecnologias Urbanas e Rurais. Campus Patos de Minas, MG, Brazil.Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Campus Patos de Minas, MG, Brazil / Universidade Federal de Uberlândia. Faculdade de Engenharia Elétrica. Laboratório de Tecnologias Urbanas e Rurais. Campus Patos de Minas, MG, Brazil.Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Campus Patos de Minas, MG, Brazil / Universidade Federal de Uberlândia. Faculdade de Engenharia Elétrica. Laboratório de Tecnologias Urbanas e Rurais. Campus Patos de Minas, MG, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Pará. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Belém, PA, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade Federal do Amazonas. Manaus, AM, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.The present study was designed as an exploratory investigation to characterize the overall profile of chemokines, growth factors, and pro-inflammatory/regulatory cytokines during acute DENV infection according to DENV-1, DENV-2, DENV-4 serotypes and age: children: 3x), except PDGF in which no fold change was observed. Moreover, despite the age ranges, DENV-1 and DENV-4 presented increased levels of VEGF, IL-6, and TNF-α in serum but decreased levels of PDGF, while DENV-2 exhibited increased levels of CXCL8, CCL4, and IL-12. Noteworthy was that DENV-2 showed increased levels of IL-12, IL-15, IL-17, IL-4, IL-9, and IL-13, and maintained an unaltered levels of PDGF at younger ages (<1–10 yo and 11–20 yo), whereas in older ages (21–40 yo and 41–75 yo), the results showed increased levels of CCL2, IL-6, and TNF-α, but lower levels of PDGF. In general, DENV infection at younger age groups exhibited more complex network immunoclusters as compared to older age groups. Multivariate analysis revealed a clustering of DENV cases according to age for a set of soluble mediators especially in subjects infected with DENV-2 serotype. Altogether, our findings demonstrate that the profile of circulating soluble mediators differs substantially in acute DENV according to age and DENV serotypes suggesting the participation of serotype-associated immune response, which may represent a potential target for development of therapeutics and could be used to assist medical directive for precise clinical management of severe cases