833 research outputs found

    Studies on proteolysis in cereals

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    Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour

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    Six hull-less barley cultivars widely grown in China were roller-milled to produce bran, shorts and flour fractions. The distribution and molecular characteristics of β-glucans from the three roller-milled fractions were investigated. The β-glucan contents in the six hull-less barley cultivars varied from 4.96% to 7.62%. For all the six cultivars, the shorts fraction contained the highest concentration of β-glucan (8.12–13.01%), followed by bran (6.15–7.58%) and flour (2.48–2.95%). Crude β-glucans were prepared from the three roller-milled fractions using aqueous sodium carbonate (pH 10). These preparations contained 45.38–71.41% β-glucan, 10.81–17.26% arabinoxylan, 2.6–9.6% protein, 2.7–9.0% starch, and 5.23–9.68% ash. Purification using α-amylase and β-xylanase in combination with pH adjustment and dialysis produced high purity β-glucan preparations (91–95%). The molecular weight (Mw) of β-glucan preparations from roller-milled fractions ranged from 117,600 to 852,400 g/mol. β-Glucan from flour had higher Mw than those from shorts and bran within the same cultivar, and β-glucan preparations from bran had the lowest Mw

    Role of PFN in ipsilateral fracture neck and shaft femur: a single solution to double fracture

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    Background: Ipsilateral, concomitant femoral neck and shaft fracture is a rare fracture pattern that presents a diagnostic and treatment challenge. A variety of management modalities have been tried to treat this complex fracture pattern ranging from conservative approach to recently introduced proximal femoral nails (PFN).Methods: We analysed the retrospective data of 25 consecutive patients who had sustained a high energy trauma who had concomitant ipsilateral fractures of the femoral shaft associated with proximal femur fractures treated with PFN between January 2010 and January 2017.Results: Average follow up period was 1.2 years. All ipsilateral neck and shaft fractures were united with proximal femur nail (PFN). Neck fracture union averaged 4.3 months (range, 3–6 months). Shaft fracture union averaged 5.4 months (range, 4–7 months). Eighteen patients (70%) had a good functional result, five patients (21%) had fair result and in two patients the result (9%) was poor.Conclusions: We observed although technically demanding, the reconstruction nail is an acceptable, cost effective and minimal invasive alternative for the management of concomitant ipsilateral fractures of the femoral neck and shaft, with a good functional outcome and fewer complications

    Giant-cell tumour of proximal radius in a 50-year-old female with wrist drop: a rare case report

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    Abstract Giant-cell tumour is a locally aggressive tumour of long bones of epiphyseal region commonly occurring in adults aged 20–40 years. Most common location is distal femur, proximal tibia, and distal radius. Different treatment options being used are curettage with bone graft or bone cement, resection with arthrodesis, reconstruction, radiation, and chemotherapy. We are reporting a case of giant-cell tumour of right proximal radius in a 50-year-old female with posterior interosseous nerve palsy. It is very rare, and only four cases have been reported in the literature. It was treated by wide margin resection with fibular grafting, titanium elastic nail system along with cancellous bone graft reconstruction

    The Asymmetric Effect of Exchange Rate on the Household Consumption Expenditures

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    Previous literature on the exchange rate and household consumption failed to examine the effect of positive and negative changes in the exchange rate on household consumption expenditures. We extended the available literature by investigating the asymmetric impacts in African emerging economies (AEE). To attain this aim, we utilized the nonlinear ARDL model, which covers both negative and positive shocks in the exchange rate. Our results indicate that movements in the exchange rate have asymmetric impacts on household consumption in all the included emerging economies, excluding Nigeria. Our findings propose recommendations for the policymakers to ensure the alignment of the optimal exchange rate for countries of emerging African economies and propose relevant policies for the countries.JEL Classification: D12, D60, I30How to Cite:Gohar, R., Chang, B. H., Uche, E., & Bhatty, K. (2023). The Asymmetric Effect of Exchange Rate on the Household Consumption Expenditures. Etikonomi, 22(1), 31–44. https://doi.org/10.15408/etk.v22i1.23428

    Transcriptomic Analysis of Peritoneal Cells in a Mouse Model of Sepsis: Confirmatory and Novel Results in Early and Late Sepsis.

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    Background The events leading to sepsis start with an invasive infection of a primary organ of the body followed by an overwhelming systemic response. Intra-abdominal infections are the second most common cause of sepsis. Peritoneal fluid is the primary site of infection in these cases. A microarray-based approach was used to study the temporal changes in cells from the peritoneal cavity of septic mice and to identify potential biomarkers and therapeutic targets for this subset of sepsis patients. Results We conducted microarray analysis of the peritoneal cells of mice infected with a non-pathogenic strain of Escherichia coli. Differentially expressed genes were identified at two early (1 h, 2 h) and one late time point (18 h). A multiplexed bead array analysis was used to confirm protein expression for several cytokines which showed differential expression at different time points based on the microarray data. Gene Ontology based hypothesis testing identified a positive bias of differentially expressed genes associated with cellular development and cell death at 2 h and 18 h respectively. Most differentially expressed genes common to all 3 time points had an immune response related function, consistent with the observation that a few bacteria are still present at 18 h. Conclusions Transcriptional regulators like PLAGL2, EBF1, TCF7, KLF10 and SBNO2, previously not described in sepsis, are differentially expressed at early and late time points. Expression pattern for key biomarkers in this study is similar to that reported in human sepsis, indicating the suitability of this model for future studies of sepsis, and the observed differences in gene expression suggest species differences or differences in the response of blood leukocytes and peritoneal leukocytes

    The Art of War in manufacturing consumer consent

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    Purpose – The purpose of this paper is to use principles from Tzu's The Art of War to develop a conceptual framework for the exploration of brand persuasion and the manufacturing of consumer consent and consumption. Design/methodology/approach – The conceptual framework is based upon formal theoretical links between Tzu's concept of engagement and the political economic aspects of Herman and Chomsky with regard to manufacturing consent and consumption engineering via use of the mass media. Findings – Within the framework the consumer is simultaneously the enemy, the manipulated target for consumption engineering and the protected people in the sales “war”. The business culture and ethical considerations of such a position are examined and hence the paper offers a novel insight into how theoretical concepts link political, social and commercial paradigms in order to gain an insight into market interventions, consumption engineering and marketing leverage. Originality/value – The paper contributes to the body of knowledge examining the relationship between Chinese and Western theoretical concepts whilst providing insight for executives seeking to leverage marketing strategy

    Recent advances in the structural and molecular biology of type IV secretion systems

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    Bacteria use type IV secretion (T4S) systems to deliver DNA and protein substrates to a diverse range of prokaryotic and eukaryotic target cells. T4S systems have great impact on human health, as they are a major source of antibiotic resistance spread among bacteria and are central to infection processes of many pathogens. Therefore, deciphering the structure and underlying translocation mechanism of T4S systems is crucial to facilitate development of new drugs. The last five years have witnessed considerable progress in unraveling the structure of T4S system subassemblies, notably that of the T4S system core complex, a large 1 MegaDalton (MDa) structure embedded in the double membrane of Gram-negative bacteria and made of 3 of the 12 T4S system components. However, the recent determination of the structure of ∼3 MDa assembly of 8 of these components has revolutionized our views of T4S system architecture and opened up new avenues of research, which are discussed in this review
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