Modeling of drug release from nanophase hydroxyapatite carriers – statistics in action

Mathematical modeling of drug release from a biomaterial, with respect to time and other factors assumes paramount importance in the field of drug delivery. In this study, doxycycline containing hydroxyapatite ceramics, placed in physiological saline for 10 days, was taken as a model. Numerical equations were derived and curve fitting was done with the observed values. The relations between various parameters and release curve were studied. The observations were verified numerically. The results of the study showed that the drug release can be quantified predictably and controlling the release is possible. Future scope of the study is also discussed

Graphene oxide functionalized with chitosan based nanoparticles as a carrier of siRNA in regulating Bcl-2 expression on Saos-2 & MG-63 cancer cells and its inflammatory response on bone marrow derived cells from mice

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Graphene oxide functionalized with chitosan based nanoparticles as a carrier of siRNA in regulating Bcl-2 expression on Saos-2 & MG-63 cancer cells and its inflammatory response on bone marrow derived cells from mice

Presently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nanoparticles were loaded with siRNA, studied for in vitro release with varying concentration & pH, and fitted to peppas model. MTT & ROS assay was used to evaluate biocompatibility of carrier and qPCR to study the inflammatory responses in particular checking gene expression of IL-6, TGF-ß, TNF-α in both RAW 264.7 and bone marrow derived macrophages. The results of study showed that release of siRNA were in a controlled fashion and effective at acidic pH that prevails on tumor site. The material was biocompatible and effective in case of Saos-2 osteosarcoma cells with a viability of 0.4±0.43 and 0.49±0.53 in case of MG-63 cells when treated with highest concentration of 100µl siRNA compared to untreated cells that were in range of 0.64±0.67 in Saos-2 and 0.61±0.63 in MG-63 cells. The results of expression of inflammatory cytokines IL-6, TGF-β & TNF-α showed negligible amount compared to control group serving the purpose of an effective carrier targeting tumor cells