177 research outputs found
The Management of Patient with Oligodontia Associated With Attention Deficit Hyperactive Disorder – A Rare Case Report
Oligodontia is characterized by missing six or more teeth in primary and permanent dentition exclusive of the third molars. Hereditary is the main etiologic factor and the principal clinical features are reduction in number of teeth, changes in the morphology of teeth, their eruption time and improper development of alveolar ridges. Prevalence of Oligodontia is 0.35% in Indian population. Treatment of Oligodontia is through a multidisciplinary approach. Attention Deficit Hyperactive Disorder (ADHD) is the most commonly diag-nosed behavioural disorder of childhood. It is a problem with inattentiveness, over-activity, impulsivity, or a combination. It affects about 3-5% of school aged children. ADHD may run in families, but the etiology is unknown. Depression, lack of sleep, learning disability and behavioural problems may appear with ADHD.The aim of this study was to describe a clinical case of 7 year old child with multiple congenital missing teeth associated with ADHD syndrome.  
Improved Serological Techniques for the Detection and Identification of Groundnut Viruses
Serology is indispensable for the detection
and identification of plant viruses.
Recently, the highly sensitive enzyme-linked
immunosorbent assay (ELISA) and immunosorbent
electron microscopy (ISEM) techniques
have been developed and may replace
some of the conventional serological methods.
The direct or standard double-antibody
sandwich (DAS) form of ELISA. as first
described by Clark and Adams (1977), has
wide applications in plant virology. An
indirect form of ELISA (I-ELISA) has recently
been developed by Barbara and Clark
(1982). The ISEM procedure developed by
Derrick (1973) combines the specificity of
serology with coventional electron microscopy.
DAS-ELISA, I-ELISA and ISEM have
been adapted for the detection and identification
of several groundnut viruses. This paper
will provide a description of each technique
and its application for the detection and characterization
of viruses occurring on groundnut
in India
Isolation and characterization of a geminivirus causing yellow mosaic disease of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) in India.
Horsegram yellow mosaic disease was shown to be caused by a geminivirus; horsegram yellow mosaic virus (HYMV). The virus could not be transmitted by mechanical sap inoculation. Leaf dip and purified virus preparations showed geminate virus particles, measuring 15-18 * 30 nm. An antiserum for HYMV was produced and in enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) tests HYMV was detected in leaf extracts of fieldinfected bambara groundnut, french bean, groundnut, limabean, mungbean, pigeonpea and soybean showing yellow mosaic symptoms. Bemisia tabaci fed on purified HYMV through a parafilm membrane transmitted the virus to all the hosts listed above but not to Ageratum conyzoides, okra, cassava, cowpea, Croton bonplandianus, Lab-lab purpureus, Malvastrum coromandalianum and tomato. No reaction was obtained in ELISA and ISEM tests between HYMV antibodies and extracts of plants diseased by whitefly-transmitted agents in India such as A. conyzoides yellow mosaic, okra yellow vein mosaic, C. bonplandianus, yellow vein mosaic, M. coromandalianum yellow vein mosaic, tomato leaf curl and cassava mosaic. HYMV was also not found to be related serologically to bean golden mosaic, virus
Indian Peanut Clump Virus Isolates: Host Range, Symptomatology, Serological Relationships, and Some Physical Properties
The symptomatology of Indian peanut clump virus (IPCV) isolates collected from five different geographical locations, Bapatla (B), Chinnaganjam (C), Hyderabad (H), Ludhiana (L), and Talod (T), differed. B-IPCV and C-IPCV were indistinguishable by host range but could be distinguished from the other isolates by symptoms on Canavalia ensiformis, Nicotiana clevelandii × glutinosa, Phaseolus vulgaris, and Vigna unguiculata. B-IPCV, C-IPCV, and T-IPCV were related serologically, but could be distinguished from H-IPCV and L-IPCV isolates in serological tests. The five isolates could not be distinguished on the basis of particle size. Each isolate contained two RNA species of 1.90 × 106 and 1.65 × 106 Mr estimated under nondenaturing conditions and a single polypeptide of 24 × 103 Mr. Significance of these findings for the diagnosis of IPCV and for screening of peanut genotypes for resistance is discussed
Using a Sweating Manikin, Controlled by a Human Physiological Model, to Evaluate Liquid Cooling Garments
This paper discusses the use of NREL's Advanced Automotive Manikin (ADAM) for evaluating NASA's liquid cooling garments for space suits
Screening Peanut Germ Plasm Lines by Enzyme-Linked Immunosorbent Assay for Seed Transmission of Peanut Mottle Virus
Enzyme-linked immunosorbent assay (ELISA) was successfully employed for detecting peanut mottle virus (PMV) in peanut seed. Virus could be detected in extracts of infected cotyledons and embryos at dilutions of up to I {3,600, although sensitivity was reduced when infected and healthy seed were mixed. PMV was detected in the testa of about 30% o(infected seed. Complete correlation was observed· among ELISA, in fectivity assays, and growing-out tests for all four· peanut cultivars tested .. PMV was seed-transmitted in all but two-namely EC 76446 (292) and NC AC 17133 (RF)-of 283 peanut germ plasm lines screened
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Overview of Vehicle Test and Analysis Results from NREL's A/C Fuel Use Reduction Research
This paper summarizes results of air-conditioning fuel use reduction technologies and techniques for light-duty vehicles evaluated over the last 10 years
Natural Occurrence of a Strain of Cowpea Mild Mottle Virus on Groundnut (Arachis hypogaea) in India)
Vein-clearing followed by downward rolling and necrosis of leaves and severe stunting of groundnut (Arachis hypogaea) plants were caused by cowpea mild mottle virus (CMMV). The virus was readily transmitted by mechanical sap inoculations to groundnut and to 10 plant species belonging to Leguminosae, Chenopodiaceae and Solanaceae. Chenopodium quinoa and Beta vulgaris were good diagnostic hosts. Diseased sap remained infective at 10–3 but not 10–4, when stored 8 to 9 days at 25 °C; for 10min at 75 °C but not 80°C. In limited tests, virus was not seed-transmitted m groundnut or soybean. Virus was transmitted by Bemisia tabaci but not by Aphis craccivora or Myzus persicae. An antiserum for CMMV was produced and virus was serologically related to CMMV reported on cowpea and groundnut crinkle virus (GCV) from West Africa. Employing carbon diffraction grating replica as a standard the modal length of virus particles to be 610 nm. Infected cells contained large number of virus particles associated with endoplasmic reticulum
Retained Surgical Items and Minimally Invasive Surgery
A retained surgical item is a surgical patient safety problem. Early reports have focused on the epidemiology of retained-item cases and the identification of patient risk factors for retention. We now know that retention has very little to do with patient characteristics and everything to do with operating room culture. It is a perception that minimally invasive procedures are safer with regard to the risk of retention. Minimally invasive surgery is still an operation where an incision is made and surgical tools are placed inside of patients, so these cases are not immune to the problem of inadvertent retention. Retained surgical items occur because of problems with multi-stakeholder operating room practices and problems in communication. The prevention of retained surgical items will therefore require practice change, knowledge, and shared information between all perioperative personnel
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