A Herb-drug interaction study: Screen the inhibitory effects of Insulin plant extract on rat liver CYP2D6 isoenzyme upon concurrent administration of Aripiprazole

Aripiprazole belongs to the atypical antipsychotic category of drug. Cytochrome2D6 (CYP2D6) is one of the prominent enzymes that play a key role in the metabolism of Aripiprazole and further formation of an active metabolite, Dehydroaripiprazole takes place. Patients under the treatment with this potent moiety have been reported with the high blood glucose level as a side effect. In addition to this, literature suggests that the leaves of Insulin plant (Costus igneus) are usually administered by diabetic patients (2-3 times) to manage the sugar level without concerning to the physicians. There might be probability while concurrent administration of (Aripiprazole and Insulin plant leaves), leaves inhibit the enzyme and ultimately Dehydroaripiprazole exhibit poor pharmacological action. Hence, the present work was done to investigate the inhibitory effect of Insulin plant extract (IPE) on CYP2D6, with the co-administration of Aripiprazole (to examine the changes in a metabolite of Aripiprazole). In order to carry out this protocol firstly, IPE was prepared by the successive extraction method. Methanolic extract of Insulin plant was found enriched with the Quercetin, which was used as a marker to carry out this study. Presence of Quercetin was confirmed with the Ultra-violet spectroscopy (UV) and High-performance liquid chromatography (HPLC) analytical methods. Characterization of Aripiprazole was done with the help of different analytical tools such as: HPLC, melting point, and UV. Aripiprazole alone and with the several dilutions of IPE were incubated using isolated rat liver microsome (RLM) and analyzed using HPLC. HPLC data demonstrated that the, mixture of IPE+Aripiprazole (herb and drug in liver microsomes), in comparison to Aripiprazole+RLM (alone drug in liver microsomes) has not shown any significant inhibition of the enzyme, and inhibitory concentration (IC50) value found to be 4.49µg/ml. Therefore this study concluded that IPE has shown safe results even at the highest clinical dose after oral administration i.e., 20-1000µg/ml and did not show any significant CYP2D6 inhibition. Nevertheless, to confirm these observations, inclusion of in vivo studies will be advantageous. As per our knowledge, this is the first attempt made on the detection of Herb- Drug interactions (HDI’S) between Insulin plant and Aripiprazole. &nbsp

Herbosome an approach to deliver Aegle marmelos extract: Formulation, characterization and stability study

Aegle marmelos fruit has tremendous potential to treat the various ailments for instance; diabetes, microbial diseases and many more. Despite, having abundant therapeutic activity poor lipid solubility and gastric instability limits the efficacy of Aegle marmelos extract (AME). With an idea to overcome these hurdles, AME was formulated as Herbosome in this study. The concept of Herbosome is gaining popularity, as it provides a sheet of lipid on the outer part of drug or extract, which helps in the bioavailability enhancement. In the present work, AME was prepared by cold extraction method and percentage yield was found to be 20% w/w. Here, Marmelosin one of the major phytoconstituent in Aegle marmelos, was used as a marker for standardization of the prepared extract. Further, preliminary phytochemical screening and thin layer chromatography (TLC) was carried out. Whereas, quantitative estimation of Marmelosin in AME by High-performance thin layer chromatography (HPTLC) was conducted. In further steps, pre-formulation parameters (effect of several processes and formulation parameters) were studied and Herbosome loaded AME was formulated by conventional technique i.e. thin film method and soyalecithin were used as phospholipids. Several parameters including, percent entrant efficiency (%EE), particle size, polydispersity index (PDI) and zeta-potential were taken into consideration for the evaluation of AME Herbosome. Later on, followed by, In vitro release of optimized formulation was studied and the formulation was able to release up to 92% even after 12 hours. After developing successful AME Herbosome, stability study was performed as per the International Conference on Harmonisation (ICH) guidelines up to the period of a month. Herbosome was found to be stable at room temperature, even between 2-4ºC. Henceforth, to confirm the efficacy of optimized formulation, In vitro studies (antidiabetic) are going on in the laboratory. Keywords: Aegle marmelos, Aegle marmelos extract, Herbosome, In vitro release, Marmelosin, Thin film method, soyalecithin, and stability stud

On the Real Time Object Detection and Tracking

Object detection and tracking is widely used for detecting motions of objects present in images and video.Since last so many decades, numerous real time object detection and tracking methods have been proposed byresearchers. The proposed methods for objects to be tracked till date require some preceding informationassociated with moving objects. In real time object detection and tracking approach segmentation is the initialtask followed by background modeling for the extraction of predefined information including shape of the objects,position in the starting frame, texture, geometry and so on for further processing of the cluster pixels and videosequence of these objects. The object detection and tracking can be applied in the fields like computerized videosurveillance, traffic monitoring, robotic vision, gesture identification, human-computer interaction, militarysurveillance system, vehicle navigation, medical imaging, biomedical image analysis and many more. In thispaper we focus detailed technical review of different methods proposed for detection and tracking of objects. Thecomparison of various techniques of detection and tracking is the purpose of this work

Almost Self-Complementary 3-Uniform Hypergraphs

It is known that self-complementary 3-uniform hypergraphs on n vertices exist if and only if n is congruent to 0, 1 or 2 modulo 4. In this paper we define an almost self-complementary 3-uniform hypergraph on n vertices and prove that it exists if and only if n is congruent to 3 modulo 4. The structure of corresponding complementing permutation is also analyzed. Further, we prove that there does not exist a regular almost self-complementary 3-uniform hypergraph on n vertices where n is congruent to 3 modulo 4, and it is proved that there exist a quasi regular almost self-complementary 3-uniform hypergraph on n vertices where n is congruent to 3 modulo 4

The Existence of Quasi Regular and Bi-Regular Self-Complementary 3-Uniform Hypergraphs

A k-uniform hypergraph H = (V; E) is called self-complementary if there is a permutation σ : V → V, called a complementing permutation, such that for every k-subset e of V, e ∈ E if and only if σ(e) ∉ E. In other words, H is isomorphic with H′ = (V ; V(k) − E). In this paper we define a bi-regular hypergraph and prove that there exists a bi-regular self-complementary 3-uniform hypergraph on n vertices if and only if n is congruent to 0 or 2 modulo 4. We also prove that there exists a quasi regular self-complementary 3-uniform hypergraph on n vertices if and only if n is congruent to 0 modulo 4

The Existence of Quasi Regular and Bi-Regular Self-Complementary 3-Uniform Hypergraphs

A k-uniform hypergraph H = (V ;E) is called self-complementary if there is a permutation σ : V → V , called a complementing permutation, such that for every k-subset e of V , e ∈ E if and only if σ(e) ∉ E. In other words, H is isomorphic with H′ = (V ; V(k) − E). In this paper we define a bi-regular hypergraph and prove that there exists a bi-regular self-complementary 3-uniform hypergraph on n vertices if and only if n is congruent to 0 or 2 modulo 4. We also prove that there exists a quasi regular self-complementary 3-uniform hypergraph on n vertices if and only if n is congruent to 0 modulo 4

Almost Self-Complementary 3-Uniform Hypergraphs

It is known that self-complementary 3-uniform hypergraphs on n vertices exist if and only if n is congruent to 0, 1 or 2 modulo 4. In this paper we define an almost self-complementary 3-uniform hypergraph on n vertices and prove that it exists if and only if n is congruent to 3 modulo 4. The structure of corresponding complementing permutation is also analyzed. Further, we prove that there does not exist a regular almost self-complementary 3-uniform hypergraph on n vertices where n is congruent to 3 modulo 4, and it is proved that there exist a quasi regular almost self-complementary 3-uniform hypergraph on n vertices where n is congruent to 3 modulo 4

Evaluation of shear bond strength of acrylic denture teeth to heat polymerized denture base resin after different surface treatments on the bonding surface of acrylic denture teeth - an in vitro study

Present study aimed to improve the bond strength of denture teeth to acrylic resin denture base by chemical or mechanical modification on the bonding surface of denture teeth. Total 40 artificial acrylic resin central incisors and lateral incisors were divided into five groups: group I, 8 samples without modification (control group); group II, 8 samples (bonding surface of teeth were treated with monomer); group III, 8 samples (bonding surface of teeth were treated with monomer and the glaze layer removed with aluminum oxide stone bur); group IV, 8 samples (bonding surface of teeth were treated with acetone); and group V, 8 samples (acetone application followed by glaze layer removed with aluminum oxide stone bonding surface of acrylic resin teeth). They were mounted on wax blocks, and the blocks were acrylized. The bond strength values were obtained by subjecting the samples to shear compressive load under universal testing machine. The results were subjected to statistical analysis. The mean value of bond strength was highest for group E (modified by aluminum oxide abrasion prior to dichloromethane application), followed by group C (modified by aluminum oxide abrasion prior to monomer application), group D (modified by dichloromethane application), group B (modified by monomer application)