Vacinação contra o Papilomavírus humano (HPV) no Brasil: histórico e desafios / Vaccination against human Papillomavirus (HPV) in Brazil: history and challenges

O câncer do colo do útero, problema de saúde pública causado pela infecção pelo Papilomavírus humano, pode ser evitado por meio da vacinação, medida que foi desafiadora desde sua implantação. Este trabalho listou marcos históricos da incorporação da vacina no Brasil sugerindo medidas para ampliação da cobertura vacinal. Os resultados indicaram a importância da realização da campanha no ambiente escolar e atividades educativas para responsáveis e adolescente

Captura de Triatoma arthurneivai (Hemiptera: Reduviidae) por nova armadilha luminosa no sudeste do Brasil

Submitted by Nuzia Santos ([email protected]) on 2014-10-27T15:23:44Z No. of bitstreams: 1 captura.pdf: 890865 bytes, checksum: c2327e8f4df9a232ebdfa3bd435d7c38 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2014-10-27T15:26:48Z (GMT) No. of bitstreams: 1 captura.pdf: 890865 bytes, checksum: c2327e8f4df9a232ebdfa3bd435d7c38 (MD5)Made available in DSpace on 2014-10-27T15:26:48Z (GMT). No. of bitstreams: 1 captura.pdf: 890865 bytes, checksum: c2327e8f4df9a232ebdfa3bd435d7c38 (MD5) Previous issue date: 2011Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Doenças Parasitárias. Diamantina, MG/ Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, BrasilUniversidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Doenças Parasitárias. Diamantina, MGUniversidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Doenças Parasitárias. Diamantina, MGFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, BrasilUniversidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Doenças Parasitárias. Diamantina, MGINTRODUÇÃO: As capturas de triatomíneos em meio silvestre são laboriosas e demoradas. Algumas armadilhas podem auxiliar nessa tarefa. Relata-se novo modelo de armadilha e captura de exemplar de espécie de triatomíneo raramente encontrada. MÉTODOS: Duas armadilhas luminosas foram instaladas em Diamantina, Estado de Minas Gerais e acompanhadas, semanalmente, durante um ano. RESULTADOS: Uma fêmea de Triatoma arthurneivai foi capturada além de outros triatomíneos. CONCLUSÕES: Um novo modelo de armadilha poderá ser empregado na captura de triatomíneos principalmente em áreas de baixa densidade. Presume-se que o centro de endemismo de Triatoma arthurneivaiseja a Cordilheira do Espinhaço.INTRODUCTION: Triatomine bug captures in the wild are laborious and time-consuming. Some traps may assist in this task. We report a new trap design and the capture of a specimen of a triatomine rarely found. METHODS: Two luminous traps were installed in the city of Diamantina, State of Minas Gerais, and surveyed weekly for a year. RESULTS: A Triatoma arthurneivai female and other triatomine bugs were caught. CONCLUSIONS: A new trap design may be used in triatomine bugs field captures, mainly in low density areas. We assume the center of endemism of Triatoma arthurneivai is the Espinhaço Mountain range

Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes

Dimethylsulfoxide (DMSO) is an amphipathic molecule composed of a polar domain characterized by the sulfinyl and two nonpolar methyl groups, for this reason it is able to solubilize polar and nonpolar substances and transpose hydrophobic barriers. DMSO is widely used to solubilize drugs of therapeutic applications and studies indicated that 10% v/v concentration did not modify culture viability when used to treat human peripheral blood mononuclear cells (PBMC). However, some DMSO concentrations could influence lymphocyte activation and present anti-inflammatory effects. Therefore, the objective of this study was to evaluate the effect of DMSO on lymphocyte activation parameters. Cell viability analysis, proliferation, and cytokine production were performed on PBMC from six healthy subjects by flow cytometry. The results indicated that 2.5% v/v DMSO concentrations did not modify lymphocytes viability. DMSO at 1% and 2% v/v concentrations reduced the relative proliferation index of lymphocytes and at 5% and 10% v/v concentrations reduced the percentage of total lymphocytes, cluster of differentiation 4+ (CD4+) T lymphocytes and CD8+ T lymphocytes interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) producers. Thus, it was concluded that DMSO has an in vitro anti-inflammatory effect by reducing lymphocyte activation demonstrated with proliferation reduction and the decrease of cytokine production

Trypan blue exclusion assay by flow cytometry

Submitted by Nuzia Santos ([email protected]) on 2015-01-28T15:27:26Z No. of bitstreams: 1 2014_003.pdf: 1135145 bytes, checksum: dc2e947d746266f16712e0890b86299c (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2015-01-28T15:27:34Z (GMT) No. of bitstreams: 1 2014_003.pdf: 1135145 bytes, checksum: dc2e947d746266f16712e0890b86299c (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2015-01-28T15:33:09Z (GMT) No. of bitstreams: 1 2014_003.pdf: 1135145 bytes, checksum: dc2e947d746266f16712e0890b86299c (MD5)Made available in DSpace on 2015-01-28T15:33:09Z (GMT). No. of bitstreams: 1 2014_003.pdf: 1135145 bytes, checksum: dc2e947d746266f16712e0890b86299c (MD5) Previous issue date: 2014Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Imunologia. Diamantina, MG, Brasil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Imunologia. Diamantina, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Farmacologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Farmacologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Farmacologia. Belo Horizonte, MG, Brasil.Fundação Osvaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração Belo Horizonte, MG, Brasil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Imunologia. Diamantina, MG, Brasil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Laboratório de Imunologia. Diamantina, MG, Brasil.Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and TB/cytoplasmic protein complexes emit fluorescence at 660 nm, which is detectable by flow cytometry using a 650-nm low-pass band filter. TB at 0.002% (w/v) was defined as the optimum concentration for distinguishing unstained living cells from fluorescent dead cells, and fluorescence emission was stable for 30 min after cell treatment. Although previous studies have shown that TB promotes green fluorescence quenching, TB at 0.002% did not interfere with green fluorescence in human live T-cells stained with anti-CD3/fluorescein isothiocyanate (FITC) monoclonal antibody. We observed a high correlation between the percentage of propidium iodide+CD3/FITC+ and TB+CD3/FITC+ cells, as well as similar double-stained cell profiles in flow cytometry dot-plot graphs. Taken together, the results indicate that a TB exclusion assay by flow cytometry can be employed as an alternative tool for quick and reliable cell viability analysis