Pezotettix giornae Microsatellite Sequences

Raw reads from the GS-FLX Roche sequencing corresponding to the 11 microsatelittes isolate

Statistical power in detection of significant genetic differentiation.

<p>Statistical power is given as calculated by Powsim 4.1 (Ryman and Palm 2006). A. Simulated sampling of Ne = 1000 into six populations reflecting sample sizes of A_VCG, A_PVS, P_VCG, P_PVS, M_Bou, M_Puy sampling groups. B. Simulated sampling of Ne = 1000 into three populations reflecting sample sizes of A, P and M overwintering strategies.</p

Result of Analysis of Molecular Variance (AMOVA) comparing the six sampling groups and the three overwintering strategies.

<p>Result of Analysis of Molecular Variance (AMOVA) comparing the six sampling groups and the three overwintering strategies.</p

Pair-wise F-statistics (F_ST) between sampling groups (a.) and overwintering strategies (b.).

<p>F_ST values in the lower matrix and significance in the upper matrix (*P<0.05; n.s. not significant)</p

Genetic diversity in sampling groups and overwintering strategies.

<p>Number of individuals sampled (N), mean number of allele per locus per sampling group or overwintering strategy (A_N), mean allelic richness corrected for sample size of 5 individuals for sampling groups and 20 individuals for overwintering strategies (R_S), expected heterozygosity under HW conditions (H_e), observed heterozygosity (H_o), inbreeding coefficient (F_IS) and its statistical significance (*P<0,05; n.s non-significant).</p

genotypic_data

Genotypic data (genepop format) of the 875 individuals sampled in the course of the study. Spatial coordinates (Lambert II), sector of origin, year of sampling and sex are provided for each individua

genotypic-data

Genotypes and spatial coordinates of the 377 P. giornae individuals sample

Coleman et al artificial barriers prevent recovery of a low mobility fish site locations

Location of survey sites where southern river blackfish tissue samples were collected from the Yarra River catchment near Melbourne, Victoria, Australia

Coleman et al artificial barriers prevent recovery of a low mobility fish raw data

Raw microsatellite data using 11 loci and 366 fin tissue samples collected from 28 southern river blackfish populations across five streams in the Yarra River catchment near Melbourne, Victoria, Australia. Individuals were sampled from 2–3 sites above and 2–3 sites below artificial barriers in each stream

Coleman et al artificial barriers prevent recovery of a low mobility fish 8 loci no less than 60mm

Raw microsatellite data for the 8 polymorphic loci only and juveniles less than 60mm removed