34 research outputs found

    Diagnostik arterieller Gefäßerkrankungen mittels Klassifikation peripherer und kardialer Impedanzsignale

    Get PDF
    Verschiedene Zeitbereichsparameter sowie aus der Wavelet-Transformation gewonnene Merkmale wurden hinsichtlich ihrer Eignung zur Frühdiagnostik peripherer Gefäßerkrankungen untersucht. Der Untersuchung liegen periphere und zentrale Impedanzsignale von 78 Probanden und 74 Dialysepatienten, die im Rahmen ihrer Therapie auf entsprechende Gefäßerkrankungen untersucht wurden, zugrunde. Zur diagnostischen Entscheidungsfindung wird ein Klassifikationsbaum verwendet. Die kombinierte Betrachtung zentraler und peripherer Kurven gestattet in Verbindung mit den Wavelet-Koeffizienten eine differenziertere Beurteilung des arteriellen Gefäßsystems als bisher bekannte, auf der Analyse von Kurven nur eines Messabschnittes beruhende Methoden

    Plant Cell Tissue Organ Cult.

    No full text
    The percentage of cells successfully transformed by Agrobacteria is usually very low (not more than 10% and usually much less; Thomas et al., 1989). In this report we show that in carrot (Daucus carota L., ssp. sativus) cell suspensions transformation efficiency was strongly improved by using cell cycle synchronized cells. Fluorodesoxyuridine (FDU) was added for 24 h to inhibit thymidine synthesis. This blocked the cell cycle at the transition from G1- to S-phase. Then the block was released by applying thymidine. A high rate of transformation was obtained when Agrobacterium tumefaciens was added concurrently with thymidine. As examples of efficient and long term foreign gene expression in transgenic cells, the reporter enzyme beta-glucuronidase (GUS) as a model as well as the major hepatitis B virus surface protein were used. Both genes were linked to the MAS promoter. In carrot cell suspensions containing the viral gene, the corresponding viral protein was produced. In roots of mature transgenic carrot plants generated through somatic embryogenesis and raised in soil as well as in callus cultures derived thereof, the viral protein was also produced

    Oncogene

    No full text
    corecore