CULTIVO DE PLEUROTUS OSTREATOROSEUS (BASIDIOMYCOTA - FUNGI) EM RESÍDUO DE TABACO

O objetivo deste experimento foi verificar a capacidade de crescimento micelial das cepas das espécies de P. ostreatoroseus em meio de cultura convencional BDA (batata-dextrose-ágar) e AET (ágar-extrato de fumo) na ausência de luz, a fim de verificar a rentabilidade no preparo do meio de cultivo alternativo utilizando resíduos de tabaco. O experimento foi desenvolvido nos laboratórios da UNIPAMPA. Para o preparo do meio convencional pesou-se 39g de BDA para 1L de água destilada, para o meio AET ferveu-se por 30 min 1 kg de resíduo de tabaco para 10L de água e do produto da fervura utilizou-se 1L para 39g de BDA, os dois meios tiveram o pH ajustados para 6 e 6,5. Em seguida, foram autoclavados por 20 min a 121 ºC. Com os resultados citados acima se pode considerar o resíduo de tabaco como um meio de cultivo para cogumelos comestíveis

The genera Phillipsia, Chlorociboria and Cookeina (Ascomycota) in Brazil and keys to the known species / Os gêneros Phillipsia, Chlorociboria e Cookeina (Ascomycota) no Brasil e chave para identificação das espécies conhecidas

Ascomycota represent the largest and most diverse group of fungi in the world and can be found in many different habitats. Unfortunately, the number registered for Brazil is low, even lower than Basidiomycota, especially due to the lack of taxonomists interested in the group. The present work aimed to study the diversity of the genera Chlorociboria, Cookeina and Phillipsia in Brazil and contribute to the easy recognition of species of these genera with the elaboration of identification keys to all species known. Using the bibliographic references citing the three genera, a list of recognized species was elaborated to Brazil and to the world. Field samples were taken in the Pampa Biome, Southern Brazil to identify occurrences in this area. Five species of Cookeina, 4 Phillipsia and 2 Chlorociboria were recognized as occurring in Brazil. Keys to all species known of Chlorociboria (27 species), Cookeina (12) and Phillipsia (26) are proposed and the distribution of all species presented

Mycophagy of Attini Ants (Hymenoptera, Formicidae, Myrmicinae) with Agaricales Mushrooms (Basidiomycota, Agaricomycetes) at Riparian Zone in Southern Brazil: Micofagia de Formigas Attini (Hymenoptera, Formicidae, Myrmicinae) com Cogumelos Agaricales (Basidiomycota, Agaricomycetes) em Zonas Ripárias no Sul do Brasil

Attini ants (Hymenoptera, Formicidae, Myrmicinae) have great diversity in exploiting food resources. However, little is known about the mycophagy involving Agaricales fungi (Basidiomycota, Agaricomycetes). Moreover, these associations are of paramount importance in riparian zones, as the interaction among soil, fauna and flora is fundamental to the maintenance of these environments. Thus, the aim of this study was to describe cases of mycophagy between ants and fungi in order to understand how these associations occur in riparian zones. To this, collections were made between 2021-2022 in the valleys of the Rio Vacacaí, Rio dos Sinos and Rio Pardo in the state of Rio Grande do Sul, Brazil. The samples were analyzed for characters macro and microscopic and identified taxonomically. Mycophagy activities of six species of ants worker were cataloged, among them Acromyrmex niger, Acromyrmex versicolor, Tranopelta gilva, Tranopelta subterranea, Pheidole flavens and Mycetosoritis hartmanni, which included in their diet the mushrooms Agaricus rufoaurantiacus, Macrocybe titans, Agrocybe underwoodii, Dactylosporina steffenii, Lepiota micropholis and Neopaxillus echinospermus, respectively. The observed interactions demonstrate the dispersal of fungal spores through ant body structures, such as legs, antennae, thorax and abdomen; basidiomata as aliment source, such as pileus, lamellae and stipe; and generalized and specialized levels of predation on various fungi structures. Our results demonstrate unprecedented mycophagous relationships and show that Agaricales fungi can be considered a food source for Attini in riparian zones in Brazil south

A relationship between fungi (Basidiomycota, Agaricomycetes, Agaricales) and nutrient content in riparian area of reforestation with Eucalyptus grandis W. Hill ex Maiden (Myrtaceae) in southern Brazil

ABSTRACT Due the tolerance in soil degraded, Eucalyptus is widely used in reforestation area. This study aims to evaluate the fungi that use Eucalyptus grandis W. Hill ex Maiden as substrate in reforestation area in southern Brazil. Fungi were identified and macronutrient and micronutrient contents were evaluated in order to understand the relationship between the fungi and the substrate. There were 200 specimens found, categorized into 25 species belonging to 10 families of Agaricales (Basidiomycota, Fungi). Substrates used by fungi were branches, roots, stems, humus, and soil. Macronutrients mean level found in fungi followed the order Ca>K>P>Mg, and micronutrients S>Fe>Mn>Cu/B>Zn. C:N ratio mean was 13:1, associated with substrate degradation potential, since the enzymatic production of fungi is affected by disposition of these nutrients. The data obtained in this study allowed a better understanding of fungi associated with the exotic arboreal substrate, and their nutritional significance in reforestation area

Identificação de espécies de cogumelos comestíveis e tóxicas da família Agaricaceae (fungos - Agaricomycetes) encontradas no Brasil / Identification of edible and toxic species of Agaricaceae mushrooms (fungi - Agaricomycetes) found in Brazil

A família Agaricaceae é composta por cogumelos geralmente facilmente reconhecíveis que são amplamente utilizados para consumo humano, pois muitas espécies são comestíveis e de tamanho avantajado. Nele existem espécies cultivadas comercialmente, como o chamado champignon, além de outras já coletadas por amadores em todo o Brasil e no mundo. No entanto, existem espécies tóxicas no grupo, eventualmente confundidas com comestíveis e outras que foram introduzidas acidental ou deliberadamente, levando a muita confusão. Para permitir a identificação de espécies nativas e introduzidas encontradas no Brasil, revisou-se o material já catalogado neste país e elaborou-se uma lista e chave de identificação para facilitar o reconhecimento e a diferenciação de espécies. De um total de 165 espécies de cogumelos Agaricaceae encontradas no Brasil, 41 são comestíveis e 12 tóxicas, para as quais é apresentada uma chave de identificação reunindo todas as espécies citadas com diâmetro de píleo acima de 4 cm, incluindo espécies sem dados sobre comestibilidade

Bryophilous Agaricomycetes (Fungi, Basidiomycota): A Review to Brazil

Bryophilous fungi have at least one stage of its life cycle linked to Bryophytes. There are few studies in relation to their taxonomy and ecology all around the world, including Brazil. The Agaricomycetes (Basidiomycota) have gained prominence worldwide and contained several species of economic interest. Based on a bibliographic review and discussion about identification methods and experimental models on this association a species list of bryophilous/Agaricomycetes found in Brazil was elaborated. In the works found among the techniques used to identify effective fungi/Bryophytes associations it can be cited: phylogenetics analysis, optical and electron microscopy, and cultivation experiments. In Brazil, four orders of Agaricomycetes (Basidiomycota), belonging to Agaricales, Boletales, Hymenochaetales, and Polyporales, with 33 species were found associated to Bryophytes in the literature. Information of the worldwide distribution of Brazilian muscicolous species and application of these groups were realized associating edibility, toxicity, and others. It was noted that in this country there is a scarcity of scientific knowledge of this subject, that needs to be better understood in terms of ecology and taxonomy

Occurrence of filamentous fungi in yerba mate discarded in the environment

Yerba mate, Ilex paraguariensis A. St. Hil. (Aquifoliaceae), waste is one of the most common byproducts generated in households in southern Brazil due to the traditional consumption of mate. However, the decomposition of this waste by fungi has not been thoroughly evaluated. In order to study the fungal succession in mate residue, 450 samples were evaluated during the autumn, winter, and spring, and kept for two months in a humid chamber (aerobic medium) for the growth of associated filamentous fungi. We morphologically identified the fungi by means of a microscopy device and taking into consideration only the surface-developed mycelia. Twenty-two genera of filamentous fungi were found, of which the Zygomycota (Rhizopus spp. and Mucor spp.) microflora deserve special attention since they were the only fungi growing in the first 19 days. After this period, the fungi Deuteromycota (mainly Aspergillus spp.) and Oomycota (Pythium spp.) develop more vigorously, supplanting the previous ones. The species of Curvularia, Fusarium, Verticillium, and Pythium are phytopathogens and end up having their inoculum source increased with the irregular disposal of this residue in nature. The presence of numerous fungal genera, which are known to be pathogenic according to the literature, highlights the importance of implementing proper waste disposal practices in the environment. Improper disposal of the yerba mate waste can result in contamination not only of yerba mate itself but also of other commercial crops. Therefore, it is imperative that greater attention be paid to the disposal of this waste