Guía metodológica para la definición y desarrollo de un data warehouse

Tesis (Ingeniero en Sistemas)--Universidad Americana, Managua, 2003El objetivo de esta tesis analizar la guía metodológica para que apoye la definición y muestrea el desarrollo de un Data Warehouse, mediante la elaboración de un estudio para adaptar los enfoques y estrategias de construcción de las metodología más utilizada hasta el año 2001 a tecnólogos de la información noveles en Data Warehousing y las estrategias para la planificación, definición y desarrollo de un Data Warehouse

A comparative gene co-expression analysis using self-organizing maps on two congener filmy ferns identifies specific desiccation tolerance mechanisms associated to their microhabitat preference

Abstract Background Filmy-ferns (Hymenophyllaceae) are poikilohydric, homoiochlorophyllous desiccation-tolerant (DT) epiphytes. They can colonize lower and upper canopy environments of humid forest. Filmy-ferns desiccate rapidly (hours), contrasting with DT angiosperms (days/weeks). It has been proposed that desiccation tolerance in filmy-ferns would be associated mainly with constitutive features rather than induced responses during dehydration. However, we hypothesize that the inter-specific differences in vertical distribution would be associated with different dynamics of gene expression within the dehydration or rehydration phases. A comparative transcriptomic analysis with an artificial neural network was done on Hymenophyllum caudiculatum (restricted to lower canopy) and Hymenophyllum dentatum (reach upper canopy) during a desiccation/rehydration cycle. Results Raw reads were assembled into 69,599 transcripts for H. dentatum and 34,726 transcripts for H. caudiculatum. Few transcripts showed significant changes in differential expression (DE). H. caudiculatum had ca. twice DE genes than H. dentatum and higher proportion of increased-and-decreased abundance of genes occurs during dehydration. In contrast, the abundance of genes in H. dentatum decreased significantly when transitioning from dehydration to rehydration. According to the artificial neural network results, H. caudiculatum enhanced osmotic responses and phenylpropanoid related pathways, whilst H. dentatum enhanced its defense system responses and protection against high light stress. Conclusions Our findings provide a deeper understanding of the mechanisms underlying the desiccation tolerance responses of two filmy ferns and the relationship between the species-specific response and the microhabitats these ferns occupy in nature

Physiological and biochemical responses to manganese toxicity in ryegrass (Lolium perenne L.) genotypes

We studied resistance to manganese (Mn) toxicity under acidic conditions and its relationship with nutrients such as calcium (Ca) and magnesium (Mg) in new perennial ryegrass (Lolium perenne L.) genotypes (One-50, Banquet-II and Halo-AR1) introduced in southern Chile, using the Nui genotype as the reference. Plants were grown in nutrient solution at increased Mn concentrations (0–750 μM) at pH 4.8, and physiological and biochemical features were determined. Under higher Mn concentration, the One-50 genotype had a significantly lower relative growth rate (RGR) of shoots and roots, whereas in the other cultivars this parameter did not change under variable Mn treatments. Increasing the Mn concentration led to an increased Mn concentration in roots and shoots, with Banquet-II and Halo-AR1 having higher Mn in roots than shoots. Shoot Mg and Ca concentrations in all genotypes (except Banquet-II) decreased concomitantly with increasing Mn applications. In contrast to the other genotypes, Banquet-II and Halo-AR1 maintained their net CO2 assimilation rate regardless of Mn treatment, whereas the chlorophyll concentration decreased in all genotypes with the exception of Banquet-II. In addition, lipid peroxidation in Banquet-II roots increased at 150 μM Mn, but decreased at higher Mn concentrations. This decrease was associated with an increase in antioxidant capacity as well as total phenol concentration. Banquet-II and Halo-AR1 appear to be the most Mn-resistant genotypes based on RGR and CO2 assimilation rate. In addition, Mn excess provoked a strong decrease in Ca and Mg concentrations in shoots of the Mn-sensitive genotype, whereas only slight variations in the Mn-resistant genotype were noted. When other evaluated parameters were taken into account, we concluded that among the perennial ryegrass genotypes introduced recently into southern Chile Banquet-II appears to be the most Mn-resistant, followed by Halo-AR1, with One-50 being the most sensitive