362 research outputs found

    Retrospective-Cost-Based Adaptive Input and State Estimation for the Ionosphere–Thermosphere

    Full text link
    Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140666/1/1.I010286.pd

    Retrospective Cost Optimization for Adaptive State Estimation, Input Estimation, and Model Refinement

    Get PDF
    AbstractRetrospective cost optimization was originally developed for adaptive control. In this paper, we show how this technique is applicable to three distinct but related problems, namely, state estimation, input estimation, and model refinement. To illustrate these techniques, we give two examples. In the first example, retrospective cost model refinement is used with synthetic data to estimate the cooling dynamics that are missing from a model of the ionosphere-thermosphere. In the second example, retrospective cost adaptive state estimation is used with data from a satellite to estimate a solar driver in the ionosphere- thermosphere, with performance gauged by using data from a second satellite

    Adaptive State Estimation for Nonminimum-Phase Systems with Uncertain Harmonic Inputs

    Full text link
    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90727/1/AIAA-2011-6315-484.pd

    Gold Standard for macromolecular crystallography diffraction data

    Get PDF
    Macromolecular crystallography (MX) is the dominant means of determining the three-dimensional structures of biological macromolecules. Over the last few decades, most MX data have been collected at synchrotron beamlines using a large number of different detectors produced by various manufacturers and taking advantage of various protocols and goniometries. These data came in their own formats: sometimes proprietary, sometimes open. The associated metadata rarely reached the degree of completeness required for data management according to Findability, Accessibility, Interoperability and Reusability (FAIR) principles. Efforts to reuse old data by other investigators or even by the original investigators some time later were often frustrated. In the culmination of an effort dating back more than two decades, a large portion of the research community concerned with high data-rate macromolecular crystallography (HDRMX) has now agreed to an updated specification of data and metadata for diffraction images produced at synchrotron light sources and X-ray free-electron lasers (XFELs). This 'Gold Standard' will facilitate the processing of data sets independent of the facility at which they were collected and enable data archiving according to FAIR principles, with a particular focus on interoperability and reusability. This agreed standard builds on the NeXus/HDF5 NXmx application definition and the International Union of Crystallography (IUCr) imgCIF/CBF dictionary, and it is compatible with major data-processing programs and pipelines. Just as with the IUCr CBF/imgCIF standard from which it arose and to which it is tied, the NeXus/HDF5 NXmx Gold Standard application definition is intended to be applicable to all detectors used for crystallography, and all hardware and software developers in the field are encouraged to adopt and contribute to the standard

    Structural and molecular interrogation of intact biological systems

    Get PDF
    Obtaining high-resolution information from a complex system, while maintaining the global perspective needed to understand system function, represents a key challenge in biology. Here we address this challenge with a method (termed CLARITY) for the transformation of intact tissue into a nanoporous hydrogel-hybridized form (crosslinked to a three-dimensional network of hydrophilic polymers) that is fully assembled but optically transparent and macromolecule-permeable. Using mouse brains, we show intact-tissue imaging of long-range projections, local circuit wiring, cellular relationships, subcellular structures, protein complexes, nucleic acids and neurotransmitters. CLARITY also enables intact-tissue in situ hybridization, immunohistochemistry with multiple rounds of staining and de-staining in non-sectioned tissue, and antibody labelling throughout the intact adult mouse brain. Finally, we show that CLARITY enables fine structural analysis of clinical samples, including non-sectioned human tissue from a neuropsychiatric-disease setting, establishing a path for the transmutation of human tissue into a stable, intact and accessible form suitable for probing structural and molecular underpinnings of physiological function and disease
    • …
    corecore