Effect of storage and preconditioning of healing rat Achilles tendon on structural and mechanical properties

Tendon tissue storage and preconditioning are often used in biomechanical experiments and whether this generates alterations in tissue properties is essential to know. The effect of storage and preconditioning on dense connective tissues, like tendons, is fairly understood. However, healing tendons are unlike and contain a loose connective tissue. Therefore, we investigated if storage of healing tendons in the fridge or freezer changed the mechanical properties compared to fresh tendons, using a pull-to-failure or a creep test. Tissue morphology and cell viability were also evaluated. Additionally, two preconditioning levels were tested. Rats underwent Achilles tendon transection and were euthanized 12 days postoperatively. Statistical analyzes were done with one-way ANOVA or Students t-test. Tissue force and stress were unaltered by storage and preconditioning compared to fresh samples, while high preconditioning increased the stiffness and modulus (p &amp;lt;= 0.007). Furthermore, both storage conditions did not modify the viscoelastic properties of the healing tendon, but altered transverse area, gap length, and water content. Cell viability was reduced after freezing. In conclusion, preconditioning on healing tissues can introduce mechanical data bias when having extensive tissue strength diversity. Storage can be used before biomechanical testing if structural properties are measured on the day of testing.Funding Agencies|Linkoping University Library</p

‘There is no heritage in Qatar’: Orientalism, colonialism and other problematic histories

This article discusses the construction of Qatari heritage in the context of pre-conceived ideas of ‘cultural heritage’ predominant in the global and regional spheres that operate in this country. It considers the location of Qatar within Middle Eastern heritage discourses and debates, and identifies productive similarities as well as unique avenues for further discussion. The authors identify the challenge of formulating methodologies that are able to recognize, accommodate, encompass and reflect local heritage dialogues and practices that exist in Qatar, which may aid in further researching the wider Arabian Peninsula, its histories and heritages