Molecular Identification and Reconstitution of Depolarization-Induced Exocytosis Monitored by Membrane Capacitance

Regulated exocytosis of neurotransmitters at synapses is fast and tightly regulated. It is unclear which proteins constitute the “minimal molecular machinery” for this process. Here, we show that a novel technique of capacitance monitoring combined with heterologous protein expression can be used to reconstitute exocytosis that is fast (<0.5 s) and triggered directly by membrane depolarization in Xenopus oocytes. Testing synaptic proteins, voltage-gated Ca(2+) channels, and using botulinum and tetanus neurotoxins established that the expression of a Ca(2+) channel together with syntaxin 1A, SNAP-25, and synaptotagmin was sufficient and necessary for the reconstitution of depolarization-induced exocytosis. Similar to synaptic exocytosis, the reconstituted release was sensitive to neurotoxins, modulated by divalent cations (Ca(2+), Ba(2+), and Sr(2+)) or channel (Lc-, N-type), and depended nonlinearly on divalent cation concentration. Because of its improved speed, native trigger, and great experimental versatility, this reconstitution assay provides a novel, promising tool to study synaptic exocytosis

Landau-Ginzburg Realization of Open String TFT.

We investigate B-type topological Landau-Ginzburg theory with one variable, with D2-brane boundary conditions. We find that the allowed brane configurations are determined in terms of the possible factorizations of the superpotential, and compute the corresponding open string chiral rings. These are characterized by bosonic and fermionic generators that satisfy certain relations. Moreover we show that the disk correlators, being continuous functions of deformation parameters, satisfy the topological sewing constraints, thereby proving consistency of the theory. In addition we show that the open string LG model is, in its content, equivalent to a certain triangulated category introduced by Kontsevich, and thus may be viewed as a concrete physical realization of it.Comment: Latex, 29p, 1 fig, refs added, various minor corrections, example section droppe