Процессно-стоимостной подход к управлению продуктами в коммерческом банке

Работа состоит из четырех частей: в первой рассмотрены теоретико-методологические основы управления банковским продуктом на основе процессно-стоимостного подхода. Во второй была осуществлена оценка банковских продуктов и услуг ПАО "Сбербанк". В третьей был разработан новый банковский продукт на основе процессно-стоимостного подхода. В процессе работы проводились теоретические исследования основ управления банковским продуктом на основе процессно-стоимостного подхода, осуществлен анализ эффективности банковских продуктов и услуг ПАО "Сбербанк". В результате исследования была произведена разработка нового банковского продукта на основе процессно-стоимостного подхода. Основные положения и выводы данной работы были сделаны в процессе прохождения преддипломной практики в ПАО "Сбербанк" и в проThe work consists of four parts: the first part addresses theoretical and methodological bases of banking product management on the basis of process-cost approach. Secondly, an assessment of banking products and services of Sberbank. In the third part has been developed a new banking product on the basis of process-cost approach. In the process of work carried out theoretical studies of the foundations of the banking product management based on a process-cost approach, the analysis of the effectiveness of banking products and services of Sberbank. The study was made to develop a new banking product on the basis of process-cost approach. The main provisions and conclusions of this work was done in the process of internship in "Sberbank" and in the process of research and analysis with a pr

The reaction of platinum(II) complexes with DNA. Kinetics of intrastrand crosslink formation in vitro.

The kinetics of the formation of bifunctional DNA platinum(II) adducts (DNA-crosslinks) have been investigated by endonuclease digestion and subsequent HPLC analysis of the soluble nucleotides and nucleotide platinum(II) adducts. The results indicate two waves of crosslinking [rate constants (0.2-0.3) min-1 and (0.015-0.025) min-1] that correlate with changes in ultra violet absorbance and ethidium bromide dependent fluorescence intensity, previously interpreted in terms of two consecutive, local conformational rearrangements of platinum-DNA (Schaller, W., Reisner, H., and Holler, E. (1987) Biochemistry 26, 943-950). The formation of crosslinks at sequences d(GpG) and d(GpNpG) follows identical kinetics. A minimal reaction mechanism is proposed for the binding of cis-diamminedichloroplatinum(II) to DNA under in vitro conditions. The approximately 3-fold higher rate for meso-[1,2-bis(2,6-dichloro-4- hydroxyphenyl)ethylenediamine]diaquaplatinum(II) in comparison to the rate for cis-diamminediaquaplatinum(II) indicates that crosslink formation is affected by the nature of the non-leaving platinum ligand(s)

Monofunctional DNA-platinum(II) adducts block frequently DNA polymerases.

The question of whether monofunctional DNA platinum(II) adducts block synthesis of DNA by purified DNA polymerases of different types and origin has been investigated by comparing the time dependence of synthesis arrest and of DNA adduct formation. Activated salmon testis DNA is used as a suitable substrate for DNA synthesis allowing to probe inhibition by platinum(II) monoadducts for the variety of inherent template-primers. Reaction amplitudes are related to defined mixtures of dichloro and chloroaqua platinum(II) complexes. It is found that (i) all investigated DNA polymerases seem arrested (100% efficiency) at bifunctional DNA adducts. (ii) human DNA polymerase beta bypasses most of the monofunctional lesions of the three platinum(II) complexes investigated. (iii) Klenow fragment is blocked by monoadducts with increasing efficiency in the order cis-diamminechloroaquaplatinum(II) (0%) less than meso-[1,2-bis(2,6- dichloro-4-hydroxyphenyl)ethylenediamine] chloroaquaplatinum(II) (50%) less than trans-diamminechloro-aquaplatinum(II) (75%). (iv) Escherichia coli DNA polymerase I, Thermus aquaticus DNA polymerase, Physarum polycephalum DNA polymerase alpha, and calf thymus DNA polymerase alpha appear to be arrested by monoadducts. According to these examples, blocking efficiencies depend on the cis/trans-stereogeometry of fixation of the carrier ligands at platinum(II) residues, on the size/chemical nature of the platin(II) carrier ligand and on the type/origin of DNA polymerase

Dichlorobis(cycloalkylamine)platinum(II) complexes. Structure activity relationship on the human MDA-MB-231 breast cancer cell line

The syntheses of dichlorobis(cycloalkylamine)platinum(II) complexes with cis and trans cycloalkylamine ligands [cis-PtCl2(C3H5NH2)2 to cis-PtCl2(C8H15NH2)2 and trans-PtCl2(C7H13NH2)2 and trans-PtCl2(C8H15NH2)2 are described. The distinction between cis and trans isomers was achieved by 1H-NMR spectroscopy. The antitumor activity was detd. on the cell proliferation of the human MDA-MB-231 breast cancer cell line during long-term drug exposure. The complexes with small cycloalkylamine ligands were inferior, those with large cycloalkylamine ligands were comparable or superior to cisplatin. All cycloalkylamine ligands were inactive. IR spectroscopic studies showed that the size of the cycloalkylamine ring does not lead to significant differences in the Pt-Cl binding strength. Therefore it is assumed that the markedly stronger antitumor activity of the higher homologs is not the result of a faster reaction with bionucleophiles such as DNA. A possible explanation of the high activity of some of the isomers is the strong lipophilicity of the complexes. This assumption was confirmed by toxicity tests against confluent cultures

Novedades corológicas en Sloanea (Elaeocarpaceae) para Colombia

Se presentan los primeros registros de Sloanea parvifructa, S. picapica, S. pittieriana y S. zuliaensis (Elaeocarpaceae)para Colombia y se complementa la descripción original de S. parvifructa al darse a conocer sus flores.Peer reviewe