Detection of Mucosal Human Papillomavirus Types 6/11 in Cutaneous Lesions from Transplant Recipients

Transplant recipients develop multiple cutaneous lesions. We have identified human papillomavirus (HPV) DNA in these lesions using three different techniques, namely polymerase chain reaction (PCR), in situ hybridization, and Southern blotting. By PCR, HPV DNA was detected in 43 of 62 samples: warts, actinic keratoses, Bowen's disease, and squamous cell carcinomas. Surprisingly, HPV 6/11, usually associated with mucosa, were frequently found in benign, premalignant, and malignant cutaneous lesions (30/43 cases). Some of these biopsies were simultaneously tested by in situ hybridization and/or Southern blotting. By in situ hybridization, HPV 6/11 were identified in two warts and one squamous cell carcinoma among 29 tissue specimens tested. Of the three samples examined by Southern blotting, HPV 6/11 were detected in one squamous cell carcinoma. In patients from a control population cutaneous biopsies did not exhibit HPV types 6/11 except in Bowen's disease; HPV types 1 or 2 were mainly found in benign warts. These findings suggest that in transplant recipients, HPV can lose their specificity towards mucosa or cutaneous epithelium. The significance of the presence of HPV 6/11 in skin lesions remains unknown

Capture of Enveloped Viruses Using Polymer Tentacles Containing Magnetic Latex Particles

International audienceA new rapid and efficient method for enveloped viruses' capture, purification and concentration has been developed. The approach is based on the use of functionalized magnetic latex particles as a carrier for capturing of the biological samples. After the capturing step, the magnetic particles are rapidly separated from the supernatant via simple permanent magnet. The nucleic acids are then directly extracted from the captured viruses on the magnetic particles using a commercial kit. The concentration methodology of viruses was first optimized for low-concentrated samples without loss of viral activity and infectivity (detected by HIV-1 p24 antigen production assay). Sensitivity of the capture assay was determined using PCR or RT-PCR method and the detection limit was found to be 10 and 16 copies for HGV and HBV respectively. The capture assay has been further used in the subtraction hybridization technique for identification of differentially expressed sequences. Finally, the lack of the tester and driver samples from the same patient by "viral positivation" of a negative serum has been circumvented using viral capture and transfer

L'expression du gene Tat de HIV-1 retarde l'apoptose de lymphocytes aviaires

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Electrostatically Driven Immobilization of Peptides onto (Maleic Anhydride- alt -methyl Vinyl Ether) Copolymers in Aqueous Media

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Supported synthesis of ferrocene modified oligonucleotides as new electroactive DNA probes

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Avian Bursal Lymphocytes Apoptosis is prevented by HIV-1 Tat gene expression

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