Gene expression analysis of bovine embryonic disc, trophoblast and parietal hypoblast at the start of gastrulation

In cattle early gastrulation-stage embryos (Stage 5), four tissues can be discerned: (i) the top layer of the embryonic disc consisting of embryonic ectoderm (EmE); (ii) the bottom layer of the disc consisting of mesoderm, endoderm and visceral hypoblast (MEH); (iii) the trophoblast (TB); and (iv) the parietal hypoblast. We performed microsurgery followed by RNA-seq to analyse the transcriptome of these four tissues as well as a developmentally earlier pre-gastrulation embryonic disc. The cattle EmE transcriptome was similar at Stages 4 and 5, characterised by the OCT4/SOX2/NANOG pluripotency network. Expression of genes associated with primordial germ cells suggest their presence in the EmE tissue at these stages. Anterior visceral hypoblast genes were transcribed in the Stage 4 disc, but no longer by Stage 5. The Stage 5 MEH layer was equally similar to mouse embryonic and extraembryonic visceral endoderm. Our data suggest that the first mesoderm to invaginate in cattle embryos is fated to become extraembryonic. TGFβ, FGF, VEGF, PDGFA, IGF2, IHH and WNT signals and receptors were expressed, however the representative members of the FGF families differed from that seen in equivalent tissues of mouse embryos. The TB transcriptome was unique and differed significantly from that of mice. FGF signalling in the TB may be autocrine with both FGFR2 and FGF2 expressed. Our data revealed a range of potential inter-tissue interactions, highlighted significant differences in early development between mice and cattle and yielded insight into the developmental events occurring at the start of gastrulation

Heat content with singular initial temperature and singular specific heat

Let (M,g) be a compact Riemannian manifold without boundary. Let D be a compact subdomain of M with smooth boundary. We examine the heat content asymptotics for the heat flow from D into M where both the initial temperature and the specific heat are permitted to have controlled singularities on the boundary of D. The operator driving the heat process is assumed to be an operator of Laplace typ

Firm Culture and Leadership as Firm Performance Predictors: a Resource-Based Perspective

In this study, we tested part of the resource-based view of the firm by examining two 'soft' resources, firm culture and top leadership, as predictors of 'hard' or bottom-line firm performance.Transformational top leadership was found to predict firm performance directly while the link between firm culture and firm performance was indirect: via transformational top leadership.Firm culture was operationalized as the employees' views about the degree of optimization of four organizational practices (job autonomy, external orientational, interdepartmental orientation, and human resource orientation).We conclude that, rather than strong cultures, firms need best organizational practices and transformational leadership.Organizational culture;Leadership firm performance;Resource-based theory of the firm

On the energy momentum dispersion in the lattice regularization

For a free scalar boson field and for U(1) gauge theory finite volume (infrared) and other corrections to the energy-momentum dispersion in the lattice regularization are investigated calculating energy eigenstates from the fall off behavior of two-point correlation functions. For small lattices the squared dispersion energy defined by $E_{\rm dis}^2=E_{\vec{k}}^2-E_0^2-4\sum_{i=1}^{d-1}\sin(k_i/2)^2$ is in both cases negative ($d$ is the Euclidean space-time dimension and $E_{\vec{k}}$ the energy of momentum $\vec{k}$ eigenstates). Observation of $E_{\rm dis}^2=0$ has been an accepted method to demonstrate the existence of a massless photon ($E_0=0$) in 4D lattice gauge theory, which we supplement here by a study of its finite size corrections. A surprise from the lattice regularization of the free field is that infrared corrections do {\it not} eliminate a difference between the groundstate energy $E_0$ and the mass parameter $M$ of the free scalar lattice action. Instead, the relation $E_0=\cosh^{-1} (1+M^2/2)$ is derived independently of the spatial lattice size.Comment: 9 pages, 2 figures. Parts of the paper have been rewritten and expanded to clarify the result

String Loop Corrections to Kahler Potentials in Orientifolds

We determine one-loop string corrections to Kahler potentials in type IIB orientifold compactifications with either N=1 or N=2 supersymmetry, including D-brane moduli, by evaluating string scattering amplitudes.Comment: 80 pages, 4 figure