Effects of genotype and age on eggshell cuticle coverage and color profile in modern laying hen strains

none3noThe aim of this research was to investigate the effects of laying hen genotype and age on eggshell cuticle deposition. A total of 4,320 brown eggs were obtained from 3 modern hen strains (A, B, and C), currently used worldwide for commercial egg production, at different intervals of age (20–30, 40–50, and 60–70 wk). Four samplings of 120 randomly collected eggs were carried out for each genotype/interval of age. Eggs were individually weighed and cuticle blue staining was used to assess quality and degree of cuticle coverage. On each egg, the eggshell color profile was assessed before and after staining using the CIE L*a*b* system and these values were used to calculate ΔE*ab. A 4-point scale visual score (VS) system was also applied to estimate the degree of cuticle coverage after staining (0 = no coverage, 1 = partial coverage, 2 = total coverage - low degree, 3 = total coverage - high degree). The effects of genotype and age and their interaction on eggshell color attributes were assessed by means of factorial ANOVA, while omnibus Chi-Square and Chi-squared Automatic Interaction Detector algorithm were applied for the analysis of VS data. Overall, both genotype and age affected the eggshell color profile as well as the degree of cuticle coverage. Hen strain A showed better cuticle deposition in comparison with B and particularly C one, being ΔE*ab values significantly higher. The VS evaluation revealed that eggs with impaired cuticle coverage degree increased with the hen age (23, 34, and 37%, respectively for 20–30, 40–50, and 60–70 wk; P < 0.05). However, a significant interaction between genotype and age was observed: transition from early to late hen age resulted in a significantly different pattern of ΔE*ab changes in each genotype. The classification tree analysis confirmed that the hen genotype has a greater effect than the age on cuticle deposition. In conclusion, considering the importance of the cuticle in table egg production, these results highlight the crucial role exerted by the genotype on eggshell cuticle coverage.openSirri F.; Zampiga M.; Berardinelli A.Sirri F.; Zampiga M.; Berardinelli A

Variability and interaction of some egg physical and eggshell quality attributes during the entire laying hen cycle

The aim of this study was to investigate the variability and relationships between some egg physical (egg weight, width, length, shape index and surface area) and eggshell parameters (weight and percentage, thickness, breaking strength, L*, a*, and b* values) during the entire laying hen cycle. A total of 8,000 eggs was collected every 5 weeks, from 30 to 81 weeks of hens age (10 samplings of 400 eggs/house), in 2 identical poultry houses equipped with enriched cages. For the statistical analysis, ANOVA, Bivariate Correlation, Principal Component Analysis (PCA) and hierarchical cluster analysis were used. An increase of egg weight, length and eggshell lightness (L*) associated with a reduction of eggshell percentage, breaking strength, and redness (a*) was observed as the hen aged (P10% of eggshell breaking strength and a*. According to the PCA, the highest changes during the laying cycle are related to egg physical parameters (32%) and to eggshell breaking strength, percentage, and thickness (26%). The egg physical parameters appeared to be strongly correlated to each other, whereas a slight correlation between eggshell breaking strength and color attributes were evidenced (-0.231 and 0.289 respectively for L* and a*; P<0.01). Hierarchical cluster analysis, based on principal components of the overall egg attributes is hereby considered, evidenced dissimilarities for eggs laid from peak production up for 39 weeks of hen age from the eggs laid afterwards. The latter group could also be divided into two subgroups, one comprising eggs laid from 44 and 53 weeks of hen age and the other from 58 weeks to the end. In conclusion, the large dataset created in this study allowed to extrapolate some robust information regarding the variability and correlations of the egg physical and eggshell quality attributes throughout the entire laying hen cycle

AP}Animal Production Technology Effects of Sinusoidal Vibrations on Quality Indices of Shell Eggs

In order to assess the effects of the sinusoidal vibrations on indices that characterise the internal quality, five samples of 30 eggs were stressed for 5 h with a constant acceleration of 0Á5 g (root mean square value, r.m.s.) at frequency linearly increasing with the time in the 5-20, 20-35, 35-50, 50-65 and 65-80 Hz ranges (one range for each sample). Vibration tests were carried out by means of an electro-dynamical shaker provided by an alveolate surface made of concrete mounted on the vibrating table. After a period of storage, Haugh unit, vitelline membrane strength, yolk index and air cell height were determined. Results showed a significant decrease (23%) of the Haugh unit in the 50-65 Hz range respect the non-vibrated eggs; a low difference in the vitelline membrane strength and the air cell height was observed between the samples vibrated at low and high frequency. In order to determine with more accuracy the frequency with the highest influence on the Haugh unit, the vitelline membrane strength and the air cell height, samples of eggs were also stressed in the 5-10, 10-15, 15-20, 50-55, 55-60 and 60-65 Hz ranges. For the Haugh unit, a very high influence of the vibration was observed in the 50-55 and 60-65 Hz range (44% of maximum decrease with respect to the non-vibrated sample). The vitelline membrane strength in the 50-55 Hz range resulted significantly lower (11%) than in the 15-20 Hz range but the highest difference (13%) was found between the 60-65 and 50-55 Hz range. The highest differences in the air cell (12%) was observed between the 10-15 and 60-65 Hz range

Physical-Mechanical Modifications of Eggs for Food-Processing During Storage

Abstract Physical-mechanical properties of egg constituents and their modifications during storage and poststorage greatly influence the efficiency of food processing, such as the separation of white and yolk by mechanical shelling. Thick albumen height, Haugh unit, yolk index and vitelline membrane-yolk system strength of eggs from Hy-Line White and Lohmann Brown hens were analyzed during 7 mo of storage at 0°C performing 3 poststorage treatments: i) immediately after refrigeration, T1; ii) after a further 6 h at 18°C after refrigeration, T2; and iii) after a week at 18°C after refrigeration, T3. For all qualitative parameters considered, this last poststorage treatment appeared to be the factor that produced the highest decrements; with respect to the first poststorage treatment, a further week at 18°C after refrigeration can involve mean decreases of about 19, 14, 14, and 16% in thick albumen height, Haugh unit, yolk index, and vitelline membrane-yolk system strength (in terms of maximum force), respectively. During about 7 mo of storage at 0°C, the latter parameter decreases, on average, by 10%. Increasing the storage time, physical-mechanical behavior was sometimes divergent from the observed trends

Adrenal involvement in the biostimulatory effect of bulls

<p>Abstract</p> <p>Background</p> <p>The objective was to evaluate if cortisol concentrations are associated with the resumption of luteal activity in postpartum, primiparous cows exposed to bulls. The hypotheses were that 1) interval from start of exposure to resumption of luteal activity; 2) proportions of cows that resumed luteal function during the exposure period; and 3) cortisol concentrations do not differ among cows exposed or not exposed to bulls (Exp. 1), and cows continuously exposed to bull or steer urine (Exp. 2).</p> <p>Methods</p> <p>In Exp. 1, 28 anovular cows were exposed (BE; n = 13) or not exposed (NE; n = 15) to bulls for 30 d at 58 d after calving. In Exp. 2, 38 anovular cows were fitted with a controlled urine delivery device at 45 d after calving and exposed continuously (24 h/d) to bull (BUE; n = 19) or steer (SUE; n = 19) urine. Length of exposure was ~64 d. Blood samples were collected from each cow on D 0 and every 3 d throughout exposure periods in both experiments and assayed for progesterone. Cortisol was assayed in samples collected on D 0, 8, 16, and 24 in Exp. 1; and, D 0, 19, 38, and 57 in Exp. 2.</p> <p>Results</p> <p>In Exp. 1, interval from the start of exposure to resumption of luteal activity was shorter (P < 0.05) for BE cows than NE cows, similarly, more (P < 0.05) BE cows than NE cows resumed luteal function during the exposure period. In Exp. 2, there was no difference in intervals from the start of exposure to resumption of luteal activity and proportions of cows that resumed luteal function during the exposure period between BUE and SUE cows. In Exp. 1, there was no difference in cortisol concentrations between BE and NE cows at the start of the experiment (D 0), however, cortisol concentrations were greater (P < 0.05) in BE cows than NE cows on D 9, 18, and 27. In Exp. 2, cortisol concentrations were higher for BUE than SUE cows on D 0 (P < 0.05), thereafter cortisol decreased (P < 0.05) but did not differ between BUE and SUE cows.</p> <p>Conclusion</p> <p>We conclude that the physical presence of bulls stimulates resumption of luteal activity and is coincident with increased cortisol concentrations, and hypothesize a possible association between adrenal activation and the biostimulatory effect of bulls.</p

Nondestructive rainbow trout (Oncorhynchus mykiss) freshness estimation by using an affordable open-ended coaxial technique

In the present work, a nondestructive device set up for a rapid and reliable freshness assessment of rainbow trout during 10 days of storage in ice was evaluated. The device was characterized by a vector network analyzer interfaced with an open coaxial probe to be placed in contact with the fish eye. The acquisition of the reflected scattering parameter (S11), which is the ratio between the amplitude of the reflected and the incident signal, was assessed in the 50 kHz-3 GHz spectral range. S11 is composed of a real part and an imaginary part, and both parts were used to predict quality index method for freshness evaluations. Partial least squares regression predictive models of the demerit scores related to fish eye attributes (eye pupil and eye shape) and the day of storage were set up. The main results showed that both the real and imaginary parts of the S11 decrease as a function of storage time. The combination with multivariate analysis allowed to set up predictive models of the storage time and the demerit scores with R-2 values up to 0.946 (root mean square error [RMSE] = 0.88 days) and 0.942 (RMSE = 3.17 demerit scores related to the fish eyes attributes), respectively (external validation). According to our results, the proposed cheap solution appears a useful tool for the freshness assessment of rainbow trout