Refinement and standardization of storage procedures for clonal crops. Global Public Goods Phase 2: Part 1. Project landscape and general status of clonal crop in vitro conservation technologies

Among the collective actions of the World Bank-funded Global Public Goods Phase II Project (GPG2), the following collaborative activity: “Refinement and standardization of storage procedures for clonal crops” was given to the CGIAR’s In Vitro Genebanks, represented by the Clonal Crop Task Force (CCTF) composed of genetic resources research staff from the four centres: Bioversity International, CIAT, CIP and IITA. These hold the in trust collections of Musa, cassava, potato, sweetpotato, yam and Andean root and tuber crops (ARTCs). The overarching aims of this activity were to: (1) review the status of vitro conservation in the context of the GPG2 project with an emphasis on the mandated clonal crops; (2) survey the facilities, storage protocols and practices of CGIAR’s clonal crop genebanks; (3) collate and review this information with a view to developing quality and risk management systems to support the production and validation of multi-crop best practice guidelines. Outputs from this activity are designated as a three part ‘trilogy’: Part I, entitled “Project landscape and general status of clonal crop in vitro conservation technologies” introduces the GPG2 project within the CGIAR landscape and overviews the status of in vitro plant conservation in the wider conservation community of practice. This part describes the role of risk and quality management for the effective maintenance of in vitro genebanks in the context of research and the development and validation of best practices

Refinement and standardization of storage procedures for clonal crops. Global Public Goods Phase 2. Part 2: Status of in vitro conservation technologies for: Andean root and tuber crops, cassava, Musa, potato, sweetpotato and yam

Among the collective actions of the World Bank-funded Global Public Goods Phase II Project (GPG2), the following collaborative activity: “Refinement and standardization of storage procedures for clonal crops” was given to the CGIAR’s In Vitro Genebanks, represented by the Clonal Crop Task Force (CCTF) composed of genetic resources research staff from the four centres: Bioversity International, CIAT, CIP and IITA. These hold the in trust collections of Musa, cassava, potato, sweetpotato, yam and Andean root and tuber crops (ARTCs). The overarching aims of this activity were to: (1) review the status of vitro conservation in the context of the GPG2 project with an emphasis on the mandated clonal crops; (2) survey the facilities, storage protocols and practices of CGIAR’s clonal crop genebanks; (3) collate and review this information with a view to developing quality and risk management systems to support the production and validation of multi-crop best practice guidelines. Outputs from this activity are designated as a three part ‘trilogy’: Part II, “Status of in vitro conservation technologies for Andean root and tuber crops, cassava, Musa, potato, sweetpotato and yam” provides a status update on the mandate clonal crops. As tasked by GPG2, it includes lessons learnt, critical point analyses and the priority research needs of CGIAR’s in vitro genebanks

Refinement and standardization of storage procedures for clonal crops. Global Public Goods Phase 2. Part 3: Multi-crop guidelines for developing in vitro conservation best practices for clonal crops

Among the collective actions of the World Bank-funded Global Public Goods Phase II Project (GPG2), the following collaborative activity: “Refinement and standardization of storage procedures for clonal crops” was given to the CGIAR’s In Vitro Genebanks, represented by the Clonal Crop Task Force (CCTF) composed of genetic resources research staff from the four centres: Bioversity International, CIAT, CIP and IITA. These hold the in trust collections of Musa, cassava, potato, sweetpotato, yam and Andean root and tuber crops (ARTCs). The overarching aims of this activity were to: (1) review the status of vitro conservation in the context of the GPG2 project with an emphasis on the mandated clonal crops; (2) survey the facilities, storage protocols and practices of CGIAR’s clonal crop genebanks; (3) collate and review this information with a view to developing quality and risk management systems to support the production and validation of multi-crop best practice guidelines. Outputs from this activity are designated as a three part ‘trilogy’: Part III, “Multi-crop guidelines for developing in vitro conservation best practices for clonal crops” is a compilation of quality and risk management best practices and guidelines from both plant and other bioresources communities. This collective knowledge provided the foundation for developing the GPG2 multi-crop best practice guidelines. They are compiled in two parts. Section I comprises general operational guidelines for quality and risk management in in vitro plant genebanks. Section II provides generic, multi-crop technical guidelines for the medium-term (slow growth) and long-term (cryopreservation) storage of crop germplasm held in In vitro active genebanks (IVAGs) and In vitro base genebanks (IVBGs) respectively

Free radical damage in stored plant germplasm

IBPGR commissioned this report to examine the implications of free radical activity for the safe conservation of plant genetic resources. This report evaluates the role of free radical damage in plant germplasm stored either as a seed or as in vitro (tissue culture) systems under cryopreservation in liquid nitrogen