ETUDE EXPERIMENTAL DE LA COMPACITE DU RESSAUT HYDRAULIQUE DANS UN CANAL TRAPEZOIDAL BRUSQUEMENT ELARGI

L'analyse bibliographique à montré que lorsque le ressaut hydraulique contrôlé est entièrement formé sur le radier du bassin amortisseur le déplacement du seuil vers l’aval ne modifie en rien la configuration du ressaut, ce qui revient à dire que la longueur  du ressaut est pratiquement assimilable à la position du seuil. Cependant, le déplacement du seuil vers l’amont  provoque la compacité du ressaut et conduit à plusieurs configurations de celui-ci, jusqu’à sa disparition complète. L’objectif principal de cette étude est de déterminer expérimentalement l’effet du seuil sur la configuration du ressaut hydraulique évoluant dans un canal trapézoïdal brusquement élargi; et de trouver des relations empiriques adimensionnelles exprimant l’effet de la compacité sur les différents paramètres du ressaut

Characterization of nanomedicines’ surface coverage using molecular probes and capillary electrophoresis

International audienceA faithful characterization of nanomedicine (NM) is needed for a better understanding of their in vivo outcomes. Size and surface charge are studied with well-established methods. However, other relevant parameters for the understanding of NM behavior in vivo remain largely inaccessible. For instance, the reactive surface of nanomedicines, which are often grafted with macromolecules to decrease their recognition by the immune system, is excluded from a systematic characterization. Yet, it is known that a subtle modification of NMs' surface characteristics (grafting density, molecular architecture and conformation of macromolecules) is at the root of major changes in the presence of biological components. In this work, a method that investigates the steric hindrance properties of the NMs’ surface coverage based on its capacity to exclude or allow adsorption of well-defined proteins was developed based on capillary electrophoresis. A series of proteins with different molecular weights (MW) were used as molecular probes to screen their adsorption behavior on nanoparticles bearing different molecular architectures at their surface. This novel strategy evaluating to some degree a functionality of NMs can bring additional information about their shell property and might allow for a better perception of their behavior in the presence of biological components. The developed method could discriminate nanoparticles with a high surface coverage excluding high MW proteins from nanoparticles with a low surface coverage that allowed high MW proteins to adsorb on their surface. The method has the potential for further standardization and automation for a routine use. It can be applied in quality control of NMs and to investigate interactions between proteins and NM in different situations

Anti-microbial and anti-oxidant activities of Illicium verum, Crataegus oxyacantha ssp. monogyna and Allium cepa red and white varieties

Illicium verum (badiane or star anise), Crataegus oxyacantha ssp monogyna (hawthorn) and Allium cepa (onion), have traditionnally been used as medicinal plants in Algeria. This study showed that the outer layer of onion is rich in flavonols with contents of 103 +/- 7.90 mu g/g DW (red variety) and 17.3 +/- 0.69 mu g/g DW (white variety). We also determined flavonols contents of 14.3 +/- 0.21 mu g/g 1.65 +/- 0.61 mu g/g for Crataegus oxyacantha ssp monogyna leaves and berries and 2.37 +/- 0.10 mu g/g for Illicium verum. Quantitative analysis of anthocyanins showed highest content in Crataegus oxyacantha ssp monogyna berries (5.11 +/- 0.266 mg/g), while, inner and outer layers of white onion had the lowest contents with 0.045 +/- 0.003 mg/g and 0.077 +/- 0.001 mg/g respectively. Flavonols extracts presented high antioxidant activity as compared with anthocyanins and standards antioxidants (ascorbic acid and quercetin). Allium cepa and Crataegus oxyacantha ssp monogyna exhibited the most effective antimicrobial activity

Chryseobacterium solincola sp nov., isolated from soil

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterium, designated strain 1YB-R12(T), was isolated from a soil sample in western Algeria. The novel isolate was heterotrophic, chemoorganotrophic, halotolerant and psychrotolerant. The temperature and pH optima for growth were 28-30 degrees C and pH 7.3-8. The bacterium tolerated up to 6% (w/v) NaCl. Cells were non-motile, non-gliding and non-spore-forming, and were characterized by a variable morphological cycle. Flexirubin-type pigments were not detected. 16S rRNA gene sequence analysis showed that strain 1YB-R12(T) occupied a distinct lineage within the genus Chryseobacterium and shared highest sequence similarity with Chryseobacterium haifense LMG 24029(T) (96.5%). The DNA G+C content of strain 1YB-R12(T) was 40.9 mol%. The predominant cellular fatty acids were anteiso-C-15:0 (41.4%) and iso-C-15:0 (14.4%). On the basis of phenotypic properties and phylogenetic distinctiveness, strain 1YB-R12(T) is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium solincola sp. nov. is proposed. The type strain is 1YB-R12(T) (=DSM 22468(T)=CCUG 55604(T))

Autonomic dysfunction and prediction of long term survival in transthyretin amyloidosis

Congress of the European-Society-of-Cardiology (ESC), Rome, ITALY, AUG 27-31, 2016International audienc