Rapid MPN-Qpcr Screening for Pathogens in Air, Soil, Water, and Agricultural Produce

A sensitive, high-throughput, and cost-effective method for screening bacterial pathogens in the environment was developed. A variety of environmental samples, including aerosols, soil of various types (sand, sand/clay mix, and clay), wastewater, and vegetable surface (modeled by tomato), were concomitantly spiked with Salmonella enterica and/or Pseudomonas aeruginosa to determine recovery rates and limits of detection. The various matrices were first enriched with a general pre-enrichment broth in a dilution series and then enumerated by most probable number (MPN) estimation using quantitative PCR for rapid screening of amplicon presence. Soil and aerosols were then tested in non-spiked environmental samples, as these matrices are prone to large experimental variation. Limit of detection in the various soil types was 1–3 colony-forming units (CFU) g[superscript −1]; on vegetable surface, 5 CFU per tomato; in treated wastewater, 5 CFU L[superscript −1]; and in aerosols, >300 CFU mL[superscript −1]. Our method accurately identified S. enterica in non-spiked environmental soil samples within a day, while traditional methods took 4 to 5 days and required sorting through biochemically and morphologically similar species. Likewise, our method successfully identified P. aeruginosa in non-spiked aerosols generated by a domestic wastewater treatment system. The obtained results suggest that the developed method presents a broad approach for the rapid, efficient, and reliable detection of relatively low densities of pathogenic organisms in challenging environmental samples.United States-Israel Binational Agricultural Research and Development Fund (Grant No. CP-9033-09)MIT International Science and Technology InitiativesKraft Foods Compan

Energy-Based Economic Development

The fields of economic development and energy policy and planning have converged in recent years to form an emerging discipline, which we term “energy-based economic development” (EBED). Despite the significant amount of stimulus funds, as well as state and local funding, that are being allocated to EBED initiatives in the United States, the emerging discipline has received scant attention in the energy, policy, and development literature. The link between energy and economic development in the literature is still theoretical, mostly focused on the need for and the potential benefits of EBED, and rarely applied. Furthermore, funding for EBED has outpaced understanding of the discipline, development of rigorous technical approaches, and meaningful ways to measure impact. Such information would not only help practitioners and policymakers more thoroughly understand the confines of the discipline and shape goals and approaches accordingly, but it would also help researchers identify, track, and evaluate a variety of activities in the field. With national and international attention focused on the convergence of these fields, researchers and practitioners have a rare opportunity to develop and implement the tools necessary to evaluate and communicate the potentially broader impacts that EBED may hold for society. If ways to leverage and sustain the injection of funds in this discipline are not identified, the opportunity may end before we can achieve either energy policy or economic development goals. In an attempt to respond to this need, this analysis explores the connection between energy and economic development, beginning with a review of the trends in each field and the goals that each seeks to achieve. On the basis of this information, we define the discipline of EBED, review the existing literature on it, and offer insights and perspectives on its emergence

FANO resonances in solid-fluid one and two dimensional systems

The gaol of this paper is to demonstrate that the propagation of acoustic waves in a single slab made of a homogeneous one dimensional (1D) solid embedded in a fluid at oblique incidence on a slab made of two dimensional (2D) rectangular rods immersed in a fluid, can exhibit transmission zeros near resonances the so-called Fano resonances

Oil palm land conversion in Pará, Brazil, from 2006-2014: evaluating the 2010 Brazilian Sustainable Palm Oil Production Program.

Global models of biophysical suitability for oil palm consistently rank Brazil as having the greatest potential for expansion, with estimates as high as 238 Mha of suitable lands. In 2010, Brazil launched the Sustainable Palm Oil Production Program (SPOPP) to incentivize oil palm development without deforestation on as much as 30Mha. Here we examine oil palm expansion before and after the SPOPP?s launch. In Par´a, the major oil palm producing state in Brazil, we analyze the extent and change in oil palm cultivation from 2006&#8722;2014 using satellite imagery, ground-truthed verification, site-based interviews, and rural environmental (land) registration data. Between 2006&#8722;2014, oil palm area (&#8805;9 ha) expanded >200% to &#8764;219 000 ha. Of the &#8764;148 000 ha of oil palm developed, &#8764;91% converted pasturelands while &#8764;8% replaced natural vegetation, including intact and secondary forests. Although >80% of all oil palm parcels rest <0.5 km from intact forests, direct conversion of intact forests declined from &#8764;4% pre-SPOPP (2006&#8722;2010) to <1% post-SPOPP (2010&#8722;2014). Despite low and declining deforestation rates associated with oil palm expansion in Par´a, our results also show a low area of oil palm development overall compared with reported land suitability. To explore potential contributing factors, we conducted semi-structured interviews with researchers, company representatives, and government officials involved in the sector to characterize the perceived factors influencing oil palm development and the role of agro-ecological suitability mapping among them. Interviews indicated that: (1) individual effects of suitability mapping efforts to encourage oil palm expansion on cleared areas, i.e. without deforestation, cannot be disentangled from pre-existing public and private deforestation reduction initiatives; and, (2) socio-economic constraints, e.g. high relative production costs and limited familiarity with this crop, appear to partially explain the major discrepancy between estimated potential suitable areas with realized oil palm development

Energy-based economic development

The fields of economic development and energy policy and planning have converged in recent years to form an emerging discipline, which we term “energy-based economic development” (EBED). Despite the significant amount of stimulus funds, as well as state and local funding, that are being allocated to EBED initiatives in the United States, the emerging discipline has received scant attention in the energy, policy, and development literature. The link between energy and economic development in the literature is still theoretical, mostly focused on the need for and the potential benefits of EBED, and rarely applied. Furthermore, funding for EBED has outpaced understanding of the discipline, development of rigorous technical approaches, and meaningful ways to measure impact. Such information would not only help practitioners and policymakers more thoroughly understand the confines of the discipline and shape goals and approaches accordingly, but it would also help researchers identify, track, and evaluate a variety of activities in the field. With national and international attention focused on the convergence of these fields, researchers and practitioners have a rare opportunity to develop and implement the tools necessary to evaluate and communicate the potentially broader impacts that EBED may hold for society. If ways to leverage and sustain the injection of funds in this discipline are not identified, the opportunity may end before we can achieve either energy policy or economic development goals. In an attempt to respond to this need, this analysis explores the connection between energy and economic development, beginning with a review of the trends in each field and the goals that each seeks to achieve. On the basis of this information, we define the discipline of EBED, review the existing literature on it, and offer insights and perspectives on its emergence

Curriculum for training course on policy impact assessment

A one-week MACSUR training course on policy impact assessment was held in March 2014 at Haifa University in Israel. The course was organised by ZALF (Hannes König, Katharina Helming) and Haifa University (Ofira Ayalon, Edan Benami, Ruslana Palatnik), targeting at the participation of Post-Docs and PhD students associated to the MACSUR consortium. The Framework for Participatory Impact Assessment (FoPIA) was used as the main method for the course to support structuring the policy impact assessment. The Israelian MACSUR case study of the Ramat Menashe Biosphere was used the test case of assessing alternative policy options and sustainability trade-offs