30 research outputs found
Dopaminergic Control of the Neurotransmitter Release in the Subthalamic Nucleus: Implications for Parkinsonâs Disease Treatment Strategies
Get PDFRecovery of total microbial RNA from lactic acid fermented foods with a high starch content
No full textAn optimized procedure for the recovery of RNA from micro-organisms involved in the fermentation of starchy foods (mainly hard-to-lyse lactic acid bacteria) is reported. Critical steps for the extraction were : cell recovery by differential centrifugation ; cell wall digestion with both mutanolysin and lysozyme ; and CTAB treatment for the elimination of starch. Digestion of starch with alpha-amylase did not improve extraction yields. The method yielded high amounts of RNA from pozol, a Mexican maize-based fermented food, and was found to extract total RNA efficiently from all the micro-organisms potentially present in these ecosystems. Both rRNA and mRNA recovered were of high quality and suitable for hybridization studies. (Résumé d'auteur
Recovery of total microbial RNA from lactic acid fermented foods with a high starch content
No full textMonitoring of microbial changes in salted cabbage (Jeolimbaechu) during recycled brining operation
No full textCharacterization of microbial population of âAlheiraâ (a traditional Portuguese fermented sausage) by PCR-DGGE and traditional cultural microbiological methods
No full textMolecular Characterization and Potential of Bacterial Species Associated with Cassava Waste
No full textDetermination of fruit origin by using 28S rDNA fingerprinting of fungi communities by PCR-DGGE: an application to Physalis fruits from Egypt, Uganda and Colombia
No full textDynamics of microbial ecology during cocoa fermentation and drying: Towards the identification of molecular markers
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