From a movement-deficient grapevine fanleaf virus to the identification of a new viral determinant of nematode transmission

Grapevine fanleaf virus (GFLV) and arabis mosaic virus (ArMV) are nepoviruses responsible for grapevine degeneration. They are specifically transmitted from grapevine to grapevine by two distinct ectoparasitic dagger nematodes of the genus Xiphinema. GFLV and ArMV move from cell to cell as virions through tubules formed into plasmodesmata by the self-assembly of the viral movement protein. Five surface-exposed regions in the coat protein called R1 to R5, which differ between the two viruses, were previously defined and exchanged to test their involvement in virus transmission, leading to the identification of region R2 as a transmission determinant. Region R4 (amino acids 258 to 264) could not be tested in transmission due to its requirement for plant systemic infection. Here, we present a fine-tuning mutagenesis of the GFLV coat protein in and around region R4 that restored the virus movement and allowed its evaluation in transmission. We show that residues T258, M260, D261, and R301 play a crucial role in virus transmission, thus representing a new viral determinant of nematode transmission

Adoption of Lightning Safety Best-Practices Policies in the Secondary School Setting

CONTEXT: Lightning-related injuries are among the top 10 causes of sport-related death at all levels of sport, including the nearly 8 million athletes participating in US secondary school sports. OBJECTIVE: To investigate the adoption of lightning safety policies and the factors that influence the development of comprehensive lightning safety policies in United States secondary schools. DESIGN: Cross-sectional study. SETTING: Secondary school. PATIENTS OR OTHER PARTICIPANTS: Athletic trainers (ATs). MAIN OUTCOME MEASURE(S): An online questionnaire was developed based on the “National Athletic Trainers' Association Position Statement: Lightning Safety for Athletics and Recreation” using a health behavior model, the precaution adoption process model, along with facilitators of and barriers to the current adoption of lightning-related policies and factors that influence the adoption of lightning policies. Precaution adoption process model stage (unaware for need, unaware if have, unengaged, undecided, decided not to act, decided to act, acting, maintaining) responses are presented as frequencies. Chi-square tests of associations and prevalence ratios with 95% CIs were calculated to compare respondents in higher and lower vulnerability states, based on data regarding lightning-related deaths. RESULTS: The response rate for this questionnaire was 13.43% (n = 365), with additional questionnaires completed via social media (n = 56). A majority of ATs reported maintaining (69%, n = 287) and acting (6.5%, n = 27) a comprehensive lightning safety policy. Approximately 1 in 4 ATs (25.1%, n = 106) described using flash to bang as an evacuation criterion. Athletic trainers practicing in more vulnerable states were more likely to adopt a lightning policy than those in less vulnerable states (57.4% versus 42.6%, prevalence ratio [95% CI] = 1.16 [1.03, 1.30]; P = .009). The most commonly cited facilitator and barrier were a requirement from a state high school athletics association and financial limitations, respectively. CONCLUSIONS: A majority of ATs related adopting (eg, maintaining and acting) the best practices for lightning safety. However, many ATs also indicated continued use of outdated methods (eg, flash to bang)

Physical, social, and biological attributes for improved understanding and prediction of wildfires: FPA FOD-Attributes dataset

Wildfires are increasingly impacting social and environmental systems in the United States (US). The ability to mitigate the adverse effects of wildfires increases with understanding of the social, physical, and biological conditions that co-occurred with or caused the wildfire ignitions and contributed to the wildfire impacts. To this end, we developed the FPA FOD-Attributes dataset, which augments the sixth version of the Fire Program Analysis Fire-Occurrence Database (FPA FOD v6) with nearly 270 attributes that coincide with the date and location of each wildfire ignition in the US. FPA FOD v6 contains information on location, jurisdiction, discovery time, cause, and final size of &gt;2.3×106 wildfires in the US between 1992 and 2020 . For each wildfire, we added physical (e.g., weather, climate, topography, and infrastructure), biological (e.g., land cover and normalized difference vegetation index), social (e.g., population density and social vulnerability index), and administrative (e.g., national and regional preparedness level and jurisdiction) attributes. This publicly available dataset can be used to answer numerous questions about the covariates associated with human- and lightning-caused wildfires. Furthermore, the FPA FOD-Attributes dataset can support descriptive, diagnostic, predictive, and prescriptive wildfire analytics, including the development of machine learning models. The FPA FOD-Attributes dataset is available at https://doi.org/10.5281/zenodo.8381129 (Pourmohamad et al., 2023).</p

e-Pilly TROP Maladies infectieuses tropicales

L’e-Pilly TROP est un ouvrage d’infectiologie tropicale destiné aux médecins et aux étudiants en médecine des pays francophones du Sud. La prise en compte des différents niveaux de la pyramide sanitaire dans ces pays le rend aussi accessible aux infirmiers des centres de santé communautaires urbains et des structures de santé intermédiaires des zones rurales. Par définition, les Pays En Développement accroissant progressivement leurs capacités de diagnostic biologique et de traitement, les outils de prise en charge correspondent aux moyens des niveaux périphériques comme à ceux des niveaux hospitaliers de référence

Influence of aerobic fitness on gastrointestinal barrier integrity and microbial translocation following a fixed-intensity military exertional heat stress test

Purpose: Exertional-heat stress adversely disrupts gastrointestinal (GI) barrier integrity, whereby subsequent microbial translocation (MT) can result in potentially serious health consequences. To date, the influence of aerobic fitness on GI barrier integrity and MT following exertional-heat stress is poorly characterised. Method: Ten untrained (UT; VO2max = 45 ± 3 ml·kg−1·min−1) and ten highly trained (HT; VO2max = 64 ± 4 ml·kg−1·min−1) males completed an ecologically valid (military) 80-min fixed-intensity exertional-heat stress test (EHST). Venous blood was drawn immediately pre- and post-EHST. GI barrier integrity was assessed using the serum dual-sugar absorption test (DSAT) and plasma Intestinal Fatty-Acid Binding Protein (I-FABP). MT was assessed using plasma Bacteroides/total 16S DNA. Results: UT experienced greater thermoregulatory, cardiovascular and perceptual strain (p < 0.05) than HT during the EHST. Serum DSAT responses were similar between the two groups (p = 0.59), although Δ I-FABP was greater (p = 0.04) in the UT (1.14 ± 1.36 ng·ml−1) versus HT (0.20 ± 0.29 ng·ml−1) group. Bacteroides/Total 16S DNA ratio was unchanged (Δ; -0.04 ± 0.18) following the EHST in the HT group, but increased (Δ; 0.19 ± 0.25) in the UT group (p = 0.05). Weekly aerobic training hours had a weak, negative correlation with Δ I-FABP and Bacteroides/total 16S DNA responses. Conclusion: When exercising at the same absolute workload, UT individuals are more susceptible to small intestinal epithelial injury and MT than HT individuals. These responses appear partially attributable to greater thermoregulatory, cardiovascular, and perceptual strain

A Historical Perspective to Inform Strategic Planning for 2020 End-of-Year Wildland Fire Response Efforts

A severe outbreak of wildfire across the US Pacific Coast during August 2020 led to persistent fire activity through the end of summer. In late September, Fire Weather Outlooks predicted higher than usual fire activity into the winter in parts of California, with concomitant elevated fire danger in the Southeastern US. To help inform the regional and national allocation of firefighting personnel and equipment, we developed visualizations of resource use during recent late season, high-demand analogs. Our visualizations provided an overview of the crew, engine, dozer, aerial resource, and incident management team usage by geographic area. While these visualizations afforded information that managers needed to support their decisions regarding resource allocation, they also revealed a potentially significant gap between resource demand and late-season availability that is only likely to increase over time due to lengthening fire seasons. This gap highlights the need for the increased assessment of suppression resource acquisition and allocation systems that, to date, have been poorly studied

Understand Daily Fire Suppression Resource Ordering and Assignment Patterns by Unsupervised Learning

Wildland fire management agencies are responsible for assigning suppression resources to control fire spread and mitigate fire risks. This study implements a principle component analysis and an association rule analysis to study wildland fire response resource requests from 2016 to 2018 in the western US to identify daily resource ordering and assignment patterns for large fire incidents. Unsupervised learning can identify patterns in the assignment of individual resources or pairs of resources. Three national Geographic Area Coordination Centers (GACCs) are studied, including California (CA), Rocky Mountain (RMC), and Southwest (SWC) at both high and low suppression preparedness levels (PLs). Substantial differences are found in resource ordering and assignment between GACCs. For example, in comparison with RMC and SWC, CA generally orders and dispatches more resources to a fire per day; CA also likely orders and assigns multiple resource types in combination. Resources are more likely assigned to a fire at higher PLs in all GACCs. This study also suggests several future research directions including studying the causal relations behind different resource ordering and assignment patterns in different regions

Water-chemistry and chloride fluctuations in the upper Floridan Aquifer in the Port Royal Sound area, South Carolina /

"Prepared in cooperation with the South Carolina Department of Natural Resources, Water Resources Division."Includes bibliographical references (p. 39-43).Mode of access: Internet