Another Formulation of the Wick's Theorem. Farewell, Pairing?

The algebraic formulation of Wick's theorem that allows one to present the vacuum or thermal averages of the chronological product of an arbitrary number of field operators as a determinant (permanent) of the matrix is proposed. Each element of the matrix is the average of the chronological product of only two operators. This formulation is extremely convenient for practical calculations in quantum field theory and statistical physics by the methods of symbolic mathematics using computers

Developmental Causation: A Set of Strict Instructions or a Self-organized Morphogenetic Field?

Two alternative versions of interpreting the developmental events are discussed. The first of them regards the development as a set of highly specific steps each of them being caused by a unique special force, or an “instruction”. By this version, nothing outside the rigidly determined chain of events is presented, and the ultimate aim of a researcher is in making a list of specific instructions. The second version is centered around the notion of an extended spatio-temporal continuum (morphogenetic field). Any developmental trajectory is now considered to be the function of this continuum’s geometry in Euclidean and/or phase space. Within the context of such an alternative we review the classical embryological data related to inductive phenomena and embryonic regulations. The contours of a morphogenetic field theory are sketched

Nonmonotonic Decay of Nonequilibrium Polariton Condensate in Direct-Gap Semiconductors

Time evolution of a nonequilibrium polariton condensate has been studied in the framework of a microscopic approach. It has been shown that due to polariton-polariton scattering a significant condensate depletion takes place in a comparatively short time interval. The condensate decay occurs in the form of multiple echo signals. Distribution-function dynamics of noncondensate polaritons have been investigated. It has been shown that at the initial stage of evolution the distribution function has the form of a bell. Then oscillations arise in the contour of the distribution function, which further transform into small chaotic ripples. The appearance of a short-wavelength wing of the distribution function has been demonstrated. We have pointed out the enhancement and then partial extinction of the sharp extra peak arising within the time interval characterized by small values of polariton condensate density and its relatively slow changes.Comment: 20 pages, LaTeX 2.09; in press in PR

Evaluation of a novel real-time polymerase chain reaction assay for identifying H3 equine influenza virus in Kazakhstan

Background and Aim: Equine influenza (EI) is a highly contagious disease that causes fever and upper respiratory tract inflammation. It is caused by influenza virus A, belonging to the Orthomyxoviridae family, with subtypes H3N8 and H7N7. This study presents data on the development of a real-time polymerase chain reaction (RT-PCR) assay using TaqMan probes to detect the H3 subtype of EI virus (EIV). Materials and Methods: The evaluation of the developed RT-PCR assay involved five strains of EIV as positive controls and ten nasopharyngeal swab samples collected from horses. RNA was isolated using the GeneJet Viral DNA and RNA Purification Kit, and primers and probes were designed using the Integrated DNA Technology PrimerQuest Tool. The assay was optimized by investigating the annealing temperature, primer and probes concentrations, sensitivity, and specificity. Sequencing was performed using the Thermo Fisher 3130 Genetic Analyzer, and the evolutionary history was inferred using the Neighbor-Joining method. Results: The designed primers and probes, targeting the H3 gene, were found to be specific to the EIV. The RT-PCR assay was capable of detecting as low as 50 femtogram (f) or 3 × 103 copies of genomic RNA. No cross-reactions were observed with other respiratory viral and bacterial pathogens, indicating the high specificity of the assay. To evaluate its effectiveness, ten nasopharyngeal swab samples collected from farms in North Kazakhstan regions during disease monitoring were analyzed. The accuracy of the analysis was confirmed by comparing the results with those obtained from a commercial RT-PCR assay for EI identification. The developed RT-PCR assay exhibited high sensitivity and specificity for detecting the EIV. Conclusion: The results demonstrate that the developed RT-PCR assay is suitable for diagnosing EI. This simple, highly sensitive, and specific assay for detecting H3 EIV can be a reliable tool for diagnosing and surveilling EI. Implementing this RT-PCR assay in veterinary practice will enhance and expedite the timely response to potential outbreaks of EI, thus positively impacting the overall epizootic well-being of EI in Kazakhstan