ISOLATION OF A BIOACTIVE PROTEIC FRACTION OF Solanum marginatum

Solanum marginatum belong to the family of Solanaceae, is widely used in traditional Mexican medicine. However, their properties have not been evaluated; so that in this study we tested some biological activities of a protein fraction of S. marginatum. For this, proteins were extracted from S. marginatum leaves, a protein fraction isolated by FPLC, protein content quantified by BCA method, an enzyme activity assay performed by Kunitz method, protein sub-fractions were identified by SDS-PAGE. The ability to capture radicals was determined by the DPPH and ABTS methods. The toxic effect was assessed with the A. salina assay while cell viability in peripheral blood mononuclear cells (PBMC) was tested by MTT method. Isolation of PF-FPLC was carried out at Rt = 2.13 min, further, separated and identified seven subfractions with molecular weights ranging from 18-112 kDa, while the optimal conditions of enzymatic activity were 37 ° C, pH 7 and 120 min. The ABTS and DPPH methods showed a radical capture percentage of 20 and 62 % respectively.PF-FPLC proteins had no toxic effect in A. salina, however, displayed a low antimicrobial effect (inhibition ≤ 35%) as well as an effect on the cellular viability of PBMC

Hydrodynamic Modeling of Flow Reversal in Micro-channel Flow Boiling

Flow boiling in micro-channels have significantly different characteristics from flow boiling in larger channels. One particular behavior of micro-channel boiling is the instability that results in oscillations and flow reversals. This has been a unique challenge for researchers, and a satisfactory solution that eliminates the instability without a substantial performance penalty has not been found. In this study, a simple analytical model based on mass and momentum conservation is developed to identify the mechanistic conditions corresponding to the conditions of flow reversal in a boiling flow in micro-channels. The results indicate that flow reversal can occur when a rapid evaporation occurs near the inlet of the channel. The model also confirms the observations of existing studies that, if a large pressure differential in the channel is imposed in combination with a throttling section before the inlet, the flow reversal can be suppressed

Music Therapy as a Treatment for Female Adolescents with Childhood Abuse

This article describes the preference to using receptive music therapy as a modality for helping an adolescent who has PTSD from childhood abuses. Adolescence is a difficult period during the life span. The second stage of separation/individuation provides challenges to almost all adolescents. This article contains a description of the adolescent behaviors of someone who is experiencing the consequences of PTSD. Along with an explanation of why receptive music therapy is an effective therapy with this population is a case study

Roles de género, calidad y satisfacción de vida en adolescentes escolarizados.

Con el objetivo de analizar la relación entre los roles de género, calidad de vida y satisfacción con la vida de jóvenes adolescentes escolarizados se realizó un estudio descriptivo transversal, como instrumento se utilizó una batería de pruebas la cual constaba de datos generales, Inventario de roles de sexo de Bem (BSRI), el Cuestionario de Salud y Bienestar en niños y adolescentes Kidscreen y la escala de Satisfacción con la vida. Participaron 450 alumnos, 222 hombres y 228 mujeres de edades entre 12 y 16 años con una media de edad de 13.5 años. Se concluye que el rol de género andrógino es el que resulta con puntajes más altos en las variables analizadas en este estudio, tanto en hombres como en mujeres. Por otra parte, los jóvenes presentan niveles altos de calidad y satisfacción de vida de forma general. Se considera el tema de roles de género relevante porque compete desde etapas iniciales y durante toda la vida de las personas, así mismo, se han ido cambiando los roles asignados e interiorizados debido a la sociedad y el contexto, así como también los temas de calidad de vida y satisfacción con la vida porque se relacionan con las esferas familiares, de salud, psicológicas, filiares y de climas escolares en los jóvenes. ABSTRCT With the aim of analyzing the relationship between gender roles, quality of life and satisfaction with the lives of young people, adolescents enrolled in school, a cross-sectional descriptive study was carried out, as an instrument used in a battery of tests, Bem's sex role inventory (BSRI), The Kidscreen Health and Wellbeing Questionnaire for Kids and Adolescents and the Life Satisfaction Scale. Participants were 450 students, 222 men and 228 women between the ages of 12 and 16 with an average age of 13.5 years. It is concluded that the gender role and the method is the result with higher scores in the variables analyzed in this study, both in men and women. On the other hand, young people present high levels of quality and life satisfaction in a general way. It is considered that the issue of roles assigned and internalized due to society and context, as well as quality issues. of life and satisfaction with life because they relate to the family, health, psychological, filial and school climates in young people

Citizen’s Police Academy

Citizen police academies have been in existence in the United States since 1985 when the Orlando Florida Police Department began its first academy. Shortly after, the State of Texas began its first citizen’s police academy at the Missouri City Police Department (TCPAAA, n.d.). Now, there are hundreds of citizen police academies all over the United States. This community oriented police program has proven to be a highly successful program that has done more for policing in bringing law enforcement agencies and the communities they serve closer together in ways that were never imaginable. Citizens participating in the citizen’s police academy get a hands-on perspective into law enforcement. Learning and working alongside police officers for the duration of the academy and beyond helps participants understand police policies and procedures. Graduates of the academy have an overwhelming positive attitude of the police department and in most cases support the agency in raising funds to help purchase much needed equipment and sponsor law enforcement training events. The positive outcomes far outweigh any issues some may rise on the costs and liability concerns

Corynebacterium endocarditis species-specific risk factors and outcomes

BACKGROUND: Corynebacterium species are recognized as uncommon agents of endocarditis, but little is known regarding species-specific risk factors and outcomes in Corynebacterium endocarditis. METHODS: Case report and Medline search of English language journals for cases of Corynebacterium endocarditis. Inclusion criteria required that cases be identified as endocarditis, having persistent Corynebacterium bacteremia, murmurs described by the authors as identifying the affected valve, or vegetations found by echocardiography or in surgical or autopsy specimens. Cases also required patient-specific information on risk factors and outcomes (age, gender, prior prosthetic valve, other prior nosocomial risk factors (infected valve, involvement of native versus prosthetic valve, need for valve replacement, and death) to be included in the analysis. Publications of Corynebacterium endocarditis which reported aggregate data were excluded. Univariate analysis was conducted with chi-square and t-tests, as appropriate, with p = 0.05 considered significant. RESULTS: 129 cases of Corynebacterium endocarditis involving nine species met inclusion criteria. Corynebacterium endocarditis typically infects the left heart of adult males and nearly one third of patients have underlying valvular disease. One quarter of patients required valve replacement and one half of patients died. Toxigenic C. diphtheriae is associated with pediatric infections (p < 0.001). Only C. amycolatum has a predilection for women (p = 0.024), while C. pseudodiphtheriticum infections are most frequent in men (p = 0.023). C. striatum, C. jeikeium and C. hemolyticum are associated with nosocomial risk factors (p < 0.001, 0.028, and 0.024, respectively). No species was found to have a predilection for any particular heart valve. C. pseudodiphtheriticum is associated with a previous prosthetic valve replacement (p = 0.004). C. jeikeium infections are more likely to require valve replacement (p = 0.026). Infections involving toxigenic C. diphtheriae and C. pseudodiphtheriticum are associated with decreased survival (p = 0.001 and 0.032, respectively). CONCLUSION: We report the first analysis of species-specific risk factors and outcomes in Corynebacterium endocarditis. In addition to species-specific associations with age, gender, prior valvular diseases, and other nosocomial risk factors, we found differences in rates of need for valve replacement and death. This review highlights the seriousness of these infections, as up to 28% of patients required valve replacement and 43.5% died

Comment on "Local accumulation times for source, diffusion, and degradation models in two and three dimensions" [J. Chem. Phys. 138, 104121 (2013)]

In a recent paper, Gordon, Muratov, and Shvartsman studied a partial differential equation (PDE) model describing radially symmetric diffusion and degradation in two and three dimensions. They paid particular attention to the local accumulation time (LAT), also known in the literature as the mean action time, which is a spatially dependent timescale that can be used to provide an estimate of the time required for the transient solution to effectively reach steady state. They presented exact results for three-dimensional applications and gave approximate results for the two-dimensional analogue. Here we make two generalizations of Gordon, Muratov, and Shvartsman’s work: (i) we present an exact expression for the LAT in any dimension and (ii) we present an exact expression for the variance of the distribution. The variance provides useful information regarding the spread about the mean that is not captured by the LAT. We conclude by describing further extensions of the model that were not considered by Gordon,Muratov, and Shvartsman. We have found that exact expressions for the LAT can also be derived for these important extensions..

Effect of gastrointestinal digestion on the bioaccessibility of phenolic compounds and antioxidant activity of fermented Aloe vera juices

Plant-based beverages are enriched by the fermentation process. However, their biocompounds are transformed during gastrointestinal digestion, improving their bioaccessibility, which is of primary importance when considering the associated health benefits. This study aimed to evaluate the effect of in vitro gastrointestinal digestion on phenolic compound bioaccessibility and antioxidant activity of novel Aloe vera juices fermented by probiotic Enterococcus faecium and Lactococcus lactis. Aloe vera juices were digested using the standardized static INFOGEST protocol. During digestion, phenolic compounds and antioxidant activity (DPPH, ABTS, and FRAP) were accessed. The digestion process was seen to significantly increase the total phenolic content of the fermented Aloe vera juices. The fermentation of Aloe vera increased the bioaccessibility of juice biocompounds, particularly for kaempferol, ellagic acid, resveratrol, hesperidin, ferulic acid, and aloin. The phenolics released during digestion were able to reduce the oxidative radicals assessed by ABTS and FRAP tests, increasing the antioxidant action in the intestine, where they are absorbed. The fermentation of Aloe vera by probiotics is an excellent process to increase the bioavailability of beverages, resulting in natural added-value functional products.This work was supported by the National Council of Science and Technology (CONACYT, Mexico) with financial support (CVU 559365). This study was also supported by the Portuguese Foundation for Science and Technology under the scope of the strategic funding of UIDB/04469/2020 unit and by LABBELS—Associate Laboratory in Biotechnology, Bioengineering, and Microelectromechanical Systems, LA/P/0029/2020. It was also supported by the European Regional Development Fund (ERDF) through the Competitiveness Factors Operational Program—Norte 2020, COMPETE, and by National Funds through the FCT—under the project AgriFood XXI (NORTE-01-0145-FEDER-000041). Clarisse Nobre acknowledges FCT for the assistant research contract 2021.01234.CEECINDinfo:eu-repo/semantics/publishedVersio