Rede de atenção a saúde: enfrentamento da violência contra mulher no município de Rio Grande/ Health care network: fighting violence against women in the municipality of Rio Grande

O estudo objetivou construir uma proposta de rede de atenção à saúde e enfrentamento à violência contra à mulher no município de Rio Grande, através de um sistema de informação, com uso dos órgãos que já atuam neste contexto, além de considerar estudos de outros modelos de redes existentes. Trata-se de um levantamento teórico-reflexivo, de abordagem qualitativacom base na identificação e análise de elementos obtidos a partir da leitura de artigos nacionais e internacionais sobre redes, além delivros impressos dos serviços que atuam no combate a violência em Rio Grande. A pesquisa ocorreu entre os meses de julho a dezembro de 2015. Com os resultados, percebe-se que, embora este município tenha distintos serviços de enfrentamento à violência contra mulher, falta conexão entre eles. Desta forma, é apresentado um modelo de fluxo de atendimento em rede, o qual interliga as informações tornando-as mais eficientes. Conclui-se que para diminuir os índices de violência contra mulher e agilizar o atendimento as mesmas, é imprescindível que os serviços de atendimento e apoio trabalhem em uma rede articulada com identificação das vítimas, prestação da assistência direta e imediata, além da punição dos agressores

Serviços de atendimento a mulheres em situação de violência doméstica/ Services for the care of women in situations of domestic violence

Objetivo: investigar o conhecimento dos enfermeiros acerca dos serviços de atendimento a mulheres em situação de violência doméstica. Método: pesquisa exploratória, descritiva, qualitativa, transversal, realizada com enfermeiros de Unidades Básicas de Saúde. Resultados: foram entrevistados 20 enfermeiros. A análise das entrevistas possibilitou a elaboração de três categorias: A busca pela Unidade de Saúde; Serviços de atenção à mulher em situação de violência doméstica; A (des)continuidade do cuidado. Conclusão: os enfermeiros possuem conhecimento acerca dos serviços disponíveis no município que atuam na assistência à mulher, na prevenção, no combate e erradicação da violência doméstica e sabem como orientá-las acerca dos direitos que possuem e dos locais que devem acessar para tal

Hyaluronate Increases Polynucleotides Effect on Human Cultured Fibroblasts

The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase repara-tion on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml

Hyaluronate Increases Polynucleotides Effect on Human Cultured Fibroblasts

The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase repara-tion on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml

A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model

Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote wound healing in an in vitro model of gingival fibroblasts. PN promoted cell growth and viability as assessed by different assays, and PN + HA, though not significantly further increasing cell growth as compared to PN, supported the formation of dense multilayered cell nodules. PN promoted fibroblasts’ clonogenic efficiency and PN + HA further enhanced the formation of more numerous dense colonies. PN + HA appeared to significantly increase the expression of collagen 1a1 and 3a1, while not affecting proteoglycans deposition. Interestingly, when tested in a scratch assay, PN + HA achieved gap closure after 48 h, while cells in the comparison groups had not completely bridged the scratch even after 96 h. Taken together, these results demonstrate that PN + HA is a promising candidate for a supportive therapy to promote soft tissue healing in the oral cavity

New Sintered Porous Scaffolds of Mg,Sr Co-Substituted Hydroxyapatite Support Growth and Differentiation of Primary Human Osteoblasts In Vitro

Strontium (Sr) and Magnesium (Mg) are bioactive ions that have been proven to exert a beneficial effect on bone; therefore, their incorporation into bone substitutes has long been viewed as a possible approach to improve tissue integration. However, the thermal instability of Mg-substituted hydroxyapatites has hitherto limited development. We previously described the creation of thermally consolidated porous constructs of Mg,Sr co-substituted apatites with adequate mechanical properties for their clinical use. The present paper describes the biocompatibility of Mg,Sr co-substituted granules using an alveolar-bone-derived primary model of human osteoblasts. Cells were cultured in the presence of different amounts of hydroxyapatite (HA), Sr-substituted HA, or MgSrHA porous macrogranules (with a size of 400–600 microns, obtained by grinding and sieving the sintered scaffolds) for three and seven days, and their viability was measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Protein content was measured using the Lowry assay at the same time points. Cell viability was not impaired by any of the tested compounds. Indirect and direct biocompatibility of these macrogranules was assessed by culturing cells in a previously conditioned medium with HA, SrHA, or MgSrHA, or in the presence of material granules. Osteoblasts formed larger and more numerous nodules around SrHA or MgSrHA granules. Furthermore, cell differentiation was evaluated by alkaline phosphatase staining of primary cells cultured in the presence of HA, SrHA, or MgSrHA granules, confirming the increased osteoconductivity of the doped materials