ATIVIDADE DE ÓLEOS ESSENCIAIS CONTRA Escherichia coli E Salmonella TYPHIMURIUM

Este trabalho possui a finalidade de avaliar o efeito dos óleos de Syzygium aromaticum ( cravo), Laurus nobilis ( e Cymbopogon winterianus ( contra as cepas de Escherichia coli ATCC 25922 e Salmonella Typhimurium ATCC 14028. As bactérias foram inoculadas em meio BHI (Brain Heart Infusion) e incubadas em estufa bacteriológica a 37ºC/24h. Posteriormente, foi realizada a inoculação destas bactérias com solução a base de óleo essencial em micropl aca de 96 poços que foi incubada em shaker orbital a 37ºC/24h em agitação de 80 rpm. Após as 24h foi realizada diluição e plaqueamento para realização das análises de CFU e DO. Cada óleo apresentou um resultado distinto, porém todos foram significativos no controle destas cepas

Toltrazuril-loaded polymeric nanocapsules as a promising approach for the preventive control of coccidiosis in poultry

Coccidiosis is a disease caused by intracellular protozoan parasites of the genus Eimeria that affect the intestinal tract of poultry. However, strain resistance and drug residue in the carcass have drawn the attention of the productive sector. The nanotechnology can improve the biological effect of drugs, reducing of administered doses and toxic effects. Due to this, toltrazuril-load polymeric nanoparticles based on Eudragit® S100 (NCt) or poly-"-caprolactone (LNCt) were developed to prevent coccidiosis in broilers. Nanoformulations were produced and showed homogeneous particle diameter distribution in the nanometer range (z-average and D (4.3) 90%. Cell viability assays using avian fibroblasts showed that LNCt presented no relevant toxicity up to 72 h. LNCt was then prophylactically administrated to chicken followed by challenge with Eimeria oocysts. The evaluation of the small intestine and cecum showed that the treatment with LNCt (3.5 mg/kg/day) in drinking water reduced the lesion scores and oocysts excretion, similar to the reference medicine containing toltrazuril (Baycox®, 7 mg/kg/day). The current study shows the potential protective use of nanoencapsulating anticoccidial drugs as a promising approach for the control of coccidiosis in poultry